Polyaniline-coated magnetic diatomite nanoparticles as a matrix for immobilizing enzymes

Cabrera, Mariana Paola; Fonseca, Taciano França da; Souza, Raquel Varela Barreto de; Assis, Caio Rodrigo Dias; Quispe-Marcatoma, J.; Maciel, Jackeline da Costa; Neri, David F.; Soria, Fernando; Carvalho, Luiz Bezerra de

doi:10.1016/j.apsusc.2018.06.238

Cited by 37 publications

(40 citation statements)

References 82 publications

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“…The magnetic bio-derivative displayed about 90% retention of specific activity after five reuses. Despite Cabrera et al [2] have reported mDE@PANI nanoparticles (~12 nm) as a promising matrix to immobilize trypsin, the immobilized derivative (mDE@PANI-TRYP) retained 75 and 60% of its initial activity after five and nine cycles of reusability, respectively. The decrease of catalytic activity could be attributed to the loss of the magnetic bio-derivative (mDE@PANI-TRYP) during the washing process after each reusability cycle.…”

Section: Micro or Nano Iron Oxide Particles?mentioning

confidence: 87%

“…The composite material (mDE-APTES) showed efficiency as a matrix to immobilize invertase. Furthermore, Cabrera et al [2] have also reported a simple, effective, and inexpensive synthesis methodology to obtain a magnetic composite made from mDE particles coated with polyaniline (mDE@PANI). The coating with PANI was carried out after preparation of the mDE particles.…”

Section: Synthesis Of Iron Oxidesmentioning

confidence: 99%

“…Cabrera et al [2] assessed by XRD the chemical composition (qualitative data) as well as the crystalline structure of magnetic diatomaceous earth coated with polyaniline (mDE@PANI) nanoparticles. The XRD pattern of the mDE@PANI sample displayed characteristic peaks for crystalline and amorphous silica along with albite, polyaniline, and magnetite.…”

Section: X-ray Diffraction (Xrd)mentioning

confidence: 99%

“…In addition, the coating with PANI did not change the chemical nature of the magnetic sample. Similarly, Cabrera et al [2] functionalized with PANI a magnetic composite from diatomaceous earth and proposed it as promising support to enzyme immobilization. Due to the great catalytic performance of the magnetic bio-derivatives, the authors evaluated the magnetic behavior by MS technique.…”

Section: Mössbauer Spectroscopy (Ms)mentioning

confidence: 99%

“…Our research group has proposed a considerable number of magnetic materials from inorganic (e.g., diatomaceous earth) or organic (e.g., azocasein) compounds and iron oxides as support to enzyme immobilization or protein purification. Recently, magnetic diatomaceous earth coated with polyaniline (mDE@PANI) exhibited good features as a matrix for covalent immobilization of three industrial enzymes (invertase, trypsin, and β-galactosidase) [2]. This magnetic composite was also promising for the treatment of boldo tea after immobilization of the enzyme tannase [3].…”

Section: Introductionmentioning

confidence: 99%

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Magnetic Bio-Derivatives: Preparation and Their Uses in Biotechnology

Cabrera¹,

Neri²,

Soria³

et al. 2019

Applied Surface Science

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In this chapter, the authors will show different proposals of magnetic bio-derivatives and its applicability in biotechnology. The historical context of immobilized enzymes, as well as highlighting the main advantages and disadvantages of each, will be mentioned. Besides, iron oxides and composite materials will be presented as support for biomolecules immobilization. Composites are effortlessly prepared including many materials capable of providing advantages to the magnetic derivatives. Enzymes covalently linked to these magnetic particles combine their catalytic properties with reaction specificity, reusability, and possible reactor construction. In addition, proteins can also be purified by these magnetic composites containing specific ligands allowing reactors and reuses too. Some characterization techniques used to study the magnetic material and derivative immobilized will be described as well. Altogether, an engaging presentation about the interesting features of magnetic bio-derivatives will highlight their uses in biotechnology field as well as in others.

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Section: Micro or Nano Iron Oxide Particles?mentioning

confidence: 87%

Section: Synthesis Of Iron Oxidesmentioning

confidence: 99%

Section: X-ray Diffraction (Xrd)mentioning

confidence: 99%

Section: Mössbauer Spectroscopy (Ms)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Magnetic Bio-Derivatives: Preparation and Their Uses in Biotechnology

Cabrera¹,

Neri²,

Soria³

et al. 2019

Applied Surface Science

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Synthesis of thermoresponsive copolymer/silica‐coated magnetite nanoparticle composite and its application for heavy metal ion recovery

Tomonaga

Hayashi

Matsuyama

et al. 2020

J of Applied Polymer Sci

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To enhance chemical stability and suppress of aggregation of magnetite nanoparticles (MNPs), which are used as a support for thermoresponsive copolymer immobilization, silica coating of the MNPs is applied via the electrooxidation method. Although the resulting silica coated-MNPs also formed aggregates, the size distribution of the aggregate shifted to smaller size range. Because of that, the surface area available for copolymer immobilization increased approximately 6.7 times at maximum as compared with that of the uncoated MNPs. It contributed to the increase of the amount of the immobilized copolymer on the silica-coated MNPs, which is approximately four times larger than that on the uncoated MNPs. Fe 3 O 4 dissolution test confirmed enhancement of chemical stability of MNPs. The thermoresponsive copolymer immobilized on the silica-coated MNPs shows the ability to recycle Cu(II) ion from Cu(II) containing solution by changing temperature with significantly shorter time than those in other thermoresponsive adsorbents in gel form.

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Octadecyl and sulfonyl modification of diatomite synergistically improved the immobilization efficiency of lipase and its application in the synthesis of pine sterol esters

Zhang,

et al. 2024

Biotechnology Journal

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Phytosterols usually have to be esterified to various phytosterol esters to avoid their disadvantages of unsatisfactory solubility and low bioavailability. The enzymatic synthesis of phytosterol esters in a solvent‐free system has advantages in terms of environmental friendliness, sustainability, and selectivity. However, the limitation of the low stability and recyclability of the lipase in the solvent‐free system, which often requires a relatively high temperature to induce the viscosity, also increased the industrial production cost. In this context, a low‐cost material, namely diatomite, was employed as the support in the immobilization of Candida rugosa lipase (CRL) due to its multiple modification sites. The Fe3O4 was also then introduced to this system for quick and simple separation via the magnetic field. Moreover, to further enhance the immobilization efficiency of diatomite, a modification strategy which involved the octadecyl and sulfonyl group for regulating the hydrophobicity and interaction between the support and lipase was successfully developed. The optimization of the ratio of the modifiers suggested that the ‐SO3H/C18 (1:1.5) performed best with an enzyme loading and enzyme activity of 84.8 mg·g−1 and 54 U·g−1, respectively. Compared with free CRL, the thermal and storage stability of CRL@OSMD was significantly improved, which lays the foundation for the catalytic synthesis of phytosterol esters in solvent‐free systems. Fortunately, a yield of 95.0% was achieved after optimizing the reaction conditions, and a yield of 70.0% can still be maintained after six cycles.

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Polyaniline-coated magnetic diatomite nanoparticles as a matrix for immobilizing enzymes

Cited by 37 publications

References 82 publications

Magnetic Bio-Derivatives: Preparation and Their Uses in Biotechnology

Magnetic Bio-Derivatives: Preparation and Their Uses in Biotechnology

Synthesis of thermoresponsive copolymer/silica‐coated magnetite nanoparticle composite and its application for heavy metal ion recovery

Octadecyl and sulfonyl modification of diatomite synergistically improved the immobilization efficiency of lipase and its application in the synthesis of pine sterol esters

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