Polyamines stimulate the phosphorylation of phosphatidylinositol in rat mast cell granules

Kurosawa, Motohiro; Uno, Daisuke; Hanawa, Kazuo; Kobayashi, Setsuo

doi:10.1111/j.1398-9995.1990.tb00494.x

Cited by 11 publications

(6 citation statements)

References 23 publications

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“…Polyamines are required for DNA synthesis and cell proliferation (58,59), and for synthesis of proline, the precursor for collagen production (60,61). Consistent with the finding of greater arginase activity in asthma in the current study, serum levels of polyamines are elevated in subjects with asthma and in experimental models of asthma (5,(62)(63)(64)(65). Taken together with previous studies, the present cross-sectional observational study identifies alterations of Arg metabolism in asthma.…”

Section: Discussionsupporting

confidence: 78%

Alterations of the Arginine Metabolome in Asthma

Lara¹,

Khatri²,

Wang³

et al. 2008

Am J Respir Crit Care Med

118

100

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Rationale: As the sole nitrogen donor in nitric oxide (NO) synthesis and key intermediate in the urea cycle, arginine and its metabolic pathways are integrally linked to cellular respiration, metabolism, and inflammation. Objectives: We hypothesized that arginine (Arg) bioavailability would be associated with airflow abnormalities and inflammation in subjects with asthma, and would be informative for asthma severity. Methods: Arg bioavailability was assessed in subjects with severe and nonsevere asthma and healthy control subjects by determination of plasma Arg relative to its metabolic products, ornithine and citrulline, and relative to methylarginine inhibitors of NO synthases, and by serum arginase activity. Inflammatory parameters, including fraction of exhaled NO (FE NO ), IgE, skin test positivity to allergens, bronchoalveolar lavage, and blood eosinophils, were also evaluated. Measurements and Main Results: Subjects with asthma had greater Arg bioavailability, but also increased Arg catabolism compared with healthy control subjects, as evidenced by higher levels of FE NO and serum arginase activity. However, Arg bioavailability was positively associated with FE NO only in healthy control subjects; Arg bioavailability was unrelated to FE NO or other inflammatory parameters in severe or nonsevere asthma. Inflammatory parameters were related to airflow obstruction and reactivity in nonsevere asthma, but not in severe asthma. Conversely, Arg bioavailability was related to airflow obstruction in severe asthma, but not in nonsevere asthma. Modeling confirmed that measures of Arg bioavailabilty predict airflow obstruction only in severe asthma. Conclusions: Unlike FE NO , Arg bioavailability is not a surrogate measure of inflammation; however, Arg bioavailability is strongly associated with airflow abnormalities in severe asthma.

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Section: Discussionsupporting

confidence: 78%

Alterations of the Arginine Metabolome in Asthma

Lara¹,

Khatri²,

Wang³

et al. 2008

Am J Respir Crit Care Med

118

100

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“…In contrast, several studies have reported that rat peritoneal tissuetype mast cells [19][20][21] as well as the rat RBL-2H3 cell line [22,23] release ROS into the extracellular milieu in response to a range of stimuli. Swindle et al, [24] reported that Pholasin was not cell-permeable and that it detected release, rather than intracellular ROS.…”

Section: Generation Of Ros By Mast Cellsmentioning

confidence: 80%

Oxidative Stress in Allergic and Inflammatory Skin Diseases

Okayama

2005

CDTIA

227

157

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The skin is exposed to endogenous and environmental pro-oxidant agents, leading to the harmful generation of reactive oxygen species (ROS). The resulting oxidative stress damages proteins, lipids, and DNA. An imbalance between ROS and antioxidants can lead to an elevated oxidative stress level. Some evidence indicates that allergic and inflammatory skin diseases like atopic dermatitis, urticaria and psoriasis are mediated by oxidative stress. For example, monocytes from patients with atopic dermatitis are primed to generate ROS in response to zymosan, a Toll-like receptor 2 (TLR2) ligand, suggesting that Staphylococcus aureus may damage lesional skin of the disease by production of ROS. Mast cells generate mainly intracellular ROS following the aggregation of FceRI; these ROS may act as secondary messengers in the induction of several biological responses. The present review summarizes the involvement of ROS in the pathogenesis of allergic and inflammatory skin diseases.

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“…Recent investigations from our laboratory indicate the presence of increased polyphosphoinositide metabolism in activated rat mast cells (5). Moreover, it has been demonstrated that naturally occurring aliphatic polyamines, such as spermine and spermidine, stimulate the phosphorylation of phosphatidylinositol in rat mast cell granules (6) and induce histamine release from rat mast cells (7). Interestingly, spermine at a concentration of 0.1 nimol/1, which in itself could not cause a significant histamine release, showed a tendency to enhance anti-IgEinduced histamine release from mast cells (7).…”

Section: Discussionmentioning

confidence: 99%

“…Aliphatic polyamines are naturally occurring cations, and their ubiquitous distribution and the complexity of the mechanisms that regulate their intracellular concentrations suggest important roles for these compounds in normal cellular growth and differentiation (4,8). Recent investigations from our laboratory indicate that naturally occurring ahphatic polyamines, such as spermine and spermidine, stimulate the phosphatidyhnositol kinase that catalyzes the formation of phosphatidyhnositol 4-phosphate from endogenous phosphatidyhnositol in rat peritoneal mast cell granules (6). They also induce a significant histamine release from rat peritoneal mast cells (7), suggesting possible involvement of polyamines in allergic reactions.…”

mentioning

confidence: 99%

Elevated levels of peripheral‐blood, naturally occurring aliphatic polyamines in bronchial asthmatic patients with active symptoms

Kurosawa

Shimizu

Tsukagoshi

et al. 1992

Allergy

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The levels of peripheral-blood, naturally occurring aliphatic polyamines, such as putrescine, spermidine, and spermine, from 21 bronchial asthmatic patients (11 atopics and 10 nonatopics) were measured by postcolumn derivatization high-performance liquid chromatography analysis. None of the patients, except the 44-year-old woman in the case report below, were given prednisolone, and they were instructed to take only regular medication during the tests. Blood was drawn from the patients in a fasting state, and the polyamine levels were compared between the times when they were free of asthmatic symptoms and when they had mild spontaneous attacks. Nine (5 atopics and 4 nonatopics), 6 (3 atopics and 3 nonatopics), and 4 (3 atopics and 1 nonatopic) out of 20 patients, when they had relatively mild asthmatic attacks, showed higher putrescine, spermidine, and spermine levels, respectively, than those of normal healthy control subjects. The levels of peripheral blood polyamines from a 44-year-old atopic bronchial asthmatic woman, who was admitted to the hospital with severe asthmatic attacks, were measured serially, and the putrescine and spermidine levels were found to be elevated during the asthmatic attacks, returning to normal levels in parallel with the clinical course. These data may suggest a role for naturally occurring aliphatic polyamines in bronchial asthma.

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Polyamines stimulate the phosphorylation of phosphatidylinositol in rat mast cell granules

Cited by 11 publications

References 23 publications

Alterations of the Arginine Metabolome in Asthma

Alterations of the Arginine Metabolome in Asthma

Oxidative Stress in Allergic and Inflammatory Skin Diseases

Elevated levels of peripheral‐blood, naturally occurring aliphatic polyamines in bronchial asthmatic patients with active symptoms

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