Polyamines oxidation is required in the symbiotic interaction Medicago truncatula–Sinorhizobium meliloti but does not participate in the regulation of polyamines level under salinity

Hidalgo-Castellanos, Javier; Marín-Peña, Agustín J.; Jiménez-Jiménez, Sara; Herrera-Cervera, José A.; López‐Gómez, Miguel

doi:10.1007/s10725-019-00508-z

Cited by 6 publications

(5 citation statements)

References 35 publications

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“…In fava beans, AG inhibited DAOs at high concentrations (5 mM) with a simultaneous reduction in GABA levels, indicating a strong relationship between PA catabolism and GABA production [ 24 ]. Interesting evidence was provided by Hidalgo-Castellanos et al [ 30 ], when AG was used to inhibit the DAO enzyme in Medicago truncatula to investigate the role of PA catabolism in nodulation. An amount of 1 mM AG was sufficient to completely inhibit the nodulation process of this leguminous plant species.…”

Section: Dao As the Main Inhibition Target Of Ag In Plantsmentioning

confidence: 99%

“…This biotic interaction between Medicago truncatula and Sinorhizobium meliloti during salt stress supported evidence that H 2 O 2 produced by PA catabolism was responsible for symbiosis formation by nodules and increased. Put levels could act as a mechanism to reduce ammonium stress in Medicago truncatula , providing evidence of the urea cycle and PA metabolism in plants [ 30 ]. Evidence of the actual mechanisms through which AG influences PA catabolism and GABA synthesis still remains to be proved.…”

Section: Dao As the Main Inhibition Target Of Ag In Plantsmentioning

confidence: 99%

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More Than a Diamine Oxidase Inhibitor: L-Aminoguanidine Modulates Polyamine-Related Abiotic Stress Responses of Plants

Köhler

Szepesi

2023

Life

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L-aminoguanidine (AG) is an inhibitor frequently used for investigating plant abiotic stress responses; however, its exact mode of action is not well understood. Many studies used this compound as a specific diamine oxidase inhibitor, whereas other studies used it for reducing nitric oxide (NO) production. Recent studies suggest its antiglycation effect; however, this remains elusive in plants. This review summarises our current knowledge about different targets of AG in plants. Our recommendation is to use AG as a modulator of polyamine-related mechanisms rather than a specific inhibitor. In the future overall investigation is needed to decipher the exact mechanisms of AG. More careful application of AG could give more insight into plant abiotic stress responses.

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Section: Dao As the Main Inhibition Target Of Ag In Plantsmentioning

confidence: 99%

Section: Dao As the Main Inhibition Target Of Ag In Plantsmentioning

confidence: 99%

More Than a Diamine Oxidase Inhibitor: L-Aminoguanidine Modulates Polyamine-Related Abiotic Stress Responses of Plants

Köhler

Szepesi

2023

Life

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“…PAs are known to play a significant role in the Rhizobium -legume symbiosis ( Hidalgo-Castellanos et al, 2019 ). In order to test the potential role of Dat in the synthesis of these compounds, HPTLC was used to determine the PA profiles in bacteroids induced by the wild-type strain Rlv UPM791 and by the dat -deficient strain UPM1458 in pea and lentil.…”

Section: Resultsmentioning

confidence: 99%

“…In other bacteria, this enzymatic activity is involved in several cellular processes, including ectoine metabolism, and synthesis of siderophores, PAs and pantothenate. As stated above, the Rlv UPM791 PAs are known to play a significant role in the Rhizobium-legume symbiosis (Hidalgo-Castellanos et al, 2019). In order to test the potential role of Dat in the synthesis of these compounds, HPTLC was used to determine the PA profiles in bacteroids induced by the wildtype strain Rlv UPM791 and by the dat-deficient strain UPM1458 in pea and lentil.…”

Section: Analysis Of the Metabolic Role Of Dat Gene In Rhizobium Legu...mentioning

confidence: 99%

A host-specific diaminobutyrate aminotransferase contributes to symbiotic performance, homoserine metabolism, and competitiveness in the Rhizobium leguminosarum/Pisum sativum system

Ballesteros-Gutiérrez,

Albareda,

Barbas

et al. 2023

Front. Microbiol.

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Rhizobium leguminosarum bv. viciae (Rlv) UPM791 effectively nodulates pea and lentil, but bacteroids contain a number of proteins differentially expressed depending on the host. One of these host-dependent proteins (C189) is similar to a diaminobutyrate-2-oxoglutarate aminotransferase (DABA-AT). DABA-AT activity was demonstrated with cell extracts and with purified protein, so C189 was renamed as Dat. The dat gene was strongly induced in the central, active area of pea nodules, but not in lentil. Mutants defective in dat were impaired in symbiotic performance with pea plants, exhibiting reduced shoot dry weight, smaller nodules, and a lower competitiveness for nodulation. In contrast, there were no significant differences between mutant and wild-type in symbiosis with lentil plants. A comparative metabolomic approach using cell-free extracts from bacteroids induced in pea and lentil showed significant differences among the strains in pea bacteroids whereas no significant differences were found in lentil. Targeted metabolomic analysis revealed that the dat mutation abolished the presence of 2,4-diaminobutyrate (DABA) in pea nodules, indicating that DABA-AT reaction is oriented toward the production of DABA from L-aspartate semialdehyde. This analysis also showed the presence of L-homoserine, a likely source of aspartate semialdehyde, in pea bacteroids but not in those induced in lentil. The dat mutant showed impaired growth when cells were grown with L-homoserine as nitrogen source. Inclusion of DABA or L-homoserine as N source suppressed pantothenate auxotropy in Rlv UPM791, suggesting DABA as source of the pantothenate precursor β-alanine. These data indicate that Rlv UPM791 Dat enzyme is part of an adaptation mechanism of this bacterium to a homoserine-rich environment such as pea nodule and rhizosphere.

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“…In soybean nodules, the addition of 200 µM Spd and Put inhibited by 37 and 54% the H + -ATPase activity, and both inward and outward ammonium channels, showing that high PAs levels have potential to reduce nitrogen supply to the plant in vivo (Whitehead et al, 2001). In addition, the M. truncatula - Sinorhizobium meliloti symbiotic system provided evidence that the H 2 O 2 produced by PAs catabolism plays a role in the inhibition of the symbiosis establishment (Hidalgo-Castellanos et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Polyamines and Legumes: Joint Stories of Stress, Nitrogen Fixation and Environment

et al. 2019

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Polyamines (PAs) are natural aliphatic amines involved in many physiological processes in almost all living organisms, including responses to abiotic stresses and microbial interactions. On other hand, the family Leguminosae constitutes an economically and ecologically key botanical group for humans, being also regarded as the most important protein source for livestock. This review presents the profuse evidence that relates changes in PAs levels during responses to biotic and abiotic stresses in model and cultivable species within Leguminosae and examines the unreviewed information regarding their potential roles in the functioning of symbiotic interactions with nitrogen-fixing bacteria and arbuscular mycorrhizae in this family. As linking plant physiological behavior with “big data” available in “omics” is an essential step to improve our understanding of legumes responses to global change, we also examined integrative MultiOmics approaches available to decrypt the interface legumes-PAs-abiotic and biotic stress interactions. These approaches are expected to accelerate the identification of stress tolerant phenotypes and the design of new biotechnological strategies to increase their yield and adaptation to marginal environments, making better use of available plant genetic resources.

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Polyamines oxidation is required in the symbiotic interaction Medicago truncatula–Sinorhizobium meliloti but does not participate in the regulation of polyamines level under salinity

Cited by 6 publications

References 35 publications

More Than a Diamine Oxidase Inhibitor: L-Aminoguanidine Modulates Polyamine-Related Abiotic Stress Responses of Plants

More Than a Diamine Oxidase Inhibitor: L-Aminoguanidine Modulates Polyamine-Related Abiotic Stress Responses of Plants

A host-specific diaminobutyrate aminotransferase contributes to symbiotic performance, homoserine metabolism, and competitiveness in the Rhizobium leguminosarum/Pisum sativum system

Polyamines and Legumes: Joint Stories of Stress, Nitrogen Fixation and Environment

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