Polyamines in Human and Rat Milk Influence Intestinal Cell Growth In Vitro
Guglielmo Capano,
Kurt J. Bloch,
Edward A. Carter
et al.
Abstract:Background:Polyamines are required for intestinal growth and development. In this study, we examined whether milk can supply the polyamines needed for growth of IEC‐6 cells, a line on non‐transformed rat intestinal crypt cells.Methods:Human, bovine, and rat milk, and cow's milk‐based infant formula were studied. Human, bovine, and rat milk were defatted and sterilized by filtration. IEC‐6 cells were stabilized in Dulbecco's modified Eagle's medium(DMEM) containing 0.5% fetal bovine serum, 5 mM L‐glutamine, 100… Show more
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