Polyamines and putreanine relax respiratory tract smooth muscle in the guinea-pig

Chideckel, Elliott W.; Fedan, Jeffrey S.; Mike, Pamela

doi:10.1016/0014-2999(85)90203-1

Cited by 22 publications

(20 citation statements)

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“…Therefore, it is expected to play an important role in cellular homeostasis in GI tract (Nilsson & Hellstrand, 1993;Tabor & Tabor, 1984). In smooth muscles, polyamines inhibit smooth muscle contractions, as shown in our study (Kim, 2007): For example, spermine causes relaxation of respiratory smooth muscle (Chideckel et al, 1985), and also inhibits spontaneous contractions of uterine smooth muscle of rat (Maruta et al, 1985). Furthermore, polyamines have also been reported to inhibit gastric emptying, therefore, ulcer formation (Aihara, 1983;Martin et al, 1986).…”

Section: Discussionmentioning

confidence: 52%

“…In general, they are associated with pathologic functions and play an important role in cell growth and differentiation (Fernández et al, 1995). Polyamines are found in various types of muscles and affect membrane excitability and motility (Chideckel et al, 1985;Swärd et al, 1994;Tsvilovskyy et al, 2004). In the case of regulation of excitability, blocking effects of polyamines are known for a variety of channels, ranging from L-type voltage dependent Ca 2+ channel (VDCCL) to inwardly rectifying K + (IRK) channels (Kim, 2007;Lopatin & Nichols, 1996).…”

Section: Introductionmentioning

confidence: 99%

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Relaxant Effect of Spermidine on Acethylcholine and High K⁺-induced Gastric Contractions of Guinea-Pig

Kim

Sim

Choi

et al. 2008

Korean J Physiol Pharmacol

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Section: Discussionmentioning

confidence: 52%

Section: Introductionmentioning

confidence: 99%

Relaxant Effect of Spermidine on Acethylcholine and High K⁺-induced Gastric Contractions of Guinea-Pig

Kim

Sim

Choi

et al. 2008

Korean J Physiol Pharmacol

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“…We have previously demonstrated that after exposure of trachealis to spermidine, K+ induces relaxation, and as is the case with the Ca2+-entry blocking drugs, the relaxation is not affected by propranolol, ouabain or indomethacin (Chideckel et al, 1985a). We decided to determine if the paradoxical relaxation of trachealis to K+ seen in the presence of Ca2+-entry blocking drugs and after spermidine could be due to a reduction in the entry of extracellular Ca2 .…”

Section: Resultsmentioning

confidence: 99%

Tetrodotoxin‐sensitive, K⁺‐induced relaxation of guinea‐pig isolated trachealis in the presence of Ca²⁺‐entry blocking drugs, Ca²⁺‐free solution and after polyamine exposure

Chideckel

Fedan

Mike

1986

British J Pharmacology

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1 We have previously observed a paradoxical relaxant effect of K+ on guinea-pig isolated trachealis after exposure to polyamines. The purpose of the present study was to evaluate whether the relaxation involved a reduction in the entry ofextracellular Ca2+. We therefore investigated the effect of K+ in the presence of Ca2+-entry blocking drugs and in the presence of Ca2+-free solution. 3 A relaxation in response to K+ was also observed in Ca2+-free solution, (with tone induced by methacholine), an effect not blocked by propranolol or ouabain. 4 Tetraethylammonium (30mM) (TEA), which ordinarily evokes contractile responses, induced trachealis relaxation in the presence of verapamil or nifedipine. The relaxation was unaltered by ouabain or propranolol. 5 Tetrodotoxin (10-6 M) (TTX) blocked 65% of the K+-induced relaxation in the presence of nifedipine and 100% of K+-induced relaxation either in a Ca2+-free solution or after polyamine exposure. TTX was without effect on TEA-induced relaxation after Ca2+-entry blocking drugs.6 Atropine (10-6 M) or hexamethonium (10-6 M) did not affect K+-induced relaxation after polyamine exposure. 7 The concentration-response curve for K+-induced contraction in normal modified Krebs-Henseleit solution was shifted to the left by TTX. 8 It is concluded: (a) K+ has a direct effect on the trachealis causing contraction and an indirect effect, mediated by neurotransmitter release, causing relaxation. This latter effect is exposed when the direct effect is inhibited by Ca2+-entry blocking drugs, Ca2+-free solution or polyamine exposure; the indirect effect is non-adrenergic, non-cholinergic and not via ganglionic transmission; (b) the TEAinduced relaxation and a component of the K+ -induced relaxation after Ca2+ blocking drugs cannot be explained by neurotransmitter release; (c) polyamines may act as naturally occurring Ca2+ antagonists.

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“…It has been shown that spermidine or spermine depressed the phasic contraction of rat uterine muscle (HASHIMOTO et al, 1973;MARUTA et al, 1985b), and tonic contraction of trachealis muscle of guinea pig (CHIDECKEL et al, 1985). Its action is very likely due to the suppression of Ca-influx.…”

Section: Postpartum Preparationsmentioning

confidence: 99%

“…Particular attention was directed to the effects of Mg, tetracaine, and spermidine which are supposed to reduce the Ca-influx (FEINSTEIN, 1965;HASHIMOTO et al, 1973;ALTURA and ALTURA, 1981;CHIDECKEL et al, 1985;MARUTA et al, 1985b;OSA and MARUTA, 1986). It is hypothesized that Ca-influx, for which the raised synthesis of prostaglandins is probably responsible, is increased in the circular myometrial tissue of postpartum rat uterus.…”

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confidence: 99%

Postpartum changes in the mechanical responses of the circular muscle of rat uterus.

Osa

Maruta

1988

Jpn.J.Physiol

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The circular muscle strips were isolated from rat uterus 10-60 h after parturition, and the electrical and mechanical responses were investigated. The muscle strips exhibited a variety of contractile activity ranging from frequent spontaneous contractions generated on an elevated muscle tone to a sustained contracture, when incubated with Mg-free Krebs solution. The muscle tone was lowered, and phasic contractions were abolished when the external Ca was removed. Muscle tone was also lowered by addition of 1.2 mM Mg, 1 mM spermidine, or 1 mM tetracaine. The membrane potential was about -25 mV in a muscle which exhibited a contracture, and the membrane was hyperpolarized by 20-25 mV by the application of 1.2 mM Mg or 1 mM spermidine. Addition of indomethacin (1.5-4.5 x 10 -5 M) caused a gradual decrease in muscle tone, and the membrane was hyperpolarized. In view of the above results, it is hypothesized that Ca-influx was increased, for which prostaglandin(s) was probably responsible, in the circular muscle of postpartum rat uterus, thereby elevating the muscle tone in Mg-free solution.

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Polyamines and putreanine relax respiratory tract smooth muscle in the guinea-pig

Cited by 22 publications

References 6 publications

Relaxant Effect of Spermidine on Acethylcholine and High K⁺-induced Gastric Contractions of Guinea-Pig

Relaxant Effect of Spermidine on Acethylcholine and High K⁺-induced Gastric Contractions of Guinea-Pig

Tetrodotoxin‐sensitive, K⁺‐induced relaxation of guinea‐pig isolated trachealis in the presence of Ca²⁺‐entry blocking drugs, Ca²⁺‐free solution and after polyamine exposure

Postpartum changes in the mechanical responses of the circular muscle of rat uterus.

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Polyamines and putreanine relax respiratory tract smooth muscle in the guinea-pig

Cited by 22 publications

References 6 publications

Relaxant Effect of Spermidine on Acethylcholine and High K+-induced Gastric Contractions of Guinea-Pig

Relaxant Effect of Spermidine on Acethylcholine and High K+-induced Gastric Contractions of Guinea-Pig

Tetrodotoxin‐sensitive, K+‐induced relaxation of guinea‐pig isolated trachealis in the presence of Ca2+‐entry blocking drugs, Ca2+‐free solution and after polyamine exposure

Postpartum changes in the mechanical responses of the circular muscle of rat uterus.

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Relaxant Effect of Spermidine on Acethylcholine and High K⁺-induced Gastric Contractions of Guinea-Pig

Relaxant Effect of Spermidine on Acethylcholine and High K⁺-induced Gastric Contractions of Guinea-Pig

Tetrodotoxin‐sensitive, K⁺‐induced relaxation of guinea‐pig isolated trachealis in the presence of Ca²⁺‐entry blocking drugs, Ca²⁺‐free solution and after polyamine exposure