Polyamine regulation of growth and chilling tolerance of rice (Oryza sativa L.) roots cultured in vitro

Lee, Tse‐Min

doi:10.1016/s0168-9452(96)04542-6

Cited by 70 publications

(45 citation statements)

References 16 publications

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“…Although 0.5 mM PUT treatment had no negative effect on biomass or photosynthesis parameters in wheat or maize plants [37], the same concentration of higher polyamines (SPD or SPM) [37] or 1 mM PUT [8] decreased the root length and increased the malondialdehyde content or H2O2 content in wheat plants. However, the exogenous application of PUT (0.01-1 mM) has also been reported to enhance the root length of rice, while SPD and SPM inhibited it [18]. In the present study, as an additive effect, the greatest growth inhibition and the highest H2O2 content and antioxidant activities were detected in the PUTpre+Cd-treated rice plants, suggesting that under these conditions PUT pretreatment provided no protection against Cd stress.…”

Section: Discussionmentioning

confidence: 42%

“…DFMO has been shown to protect plants from pathogenic fungi [14] and larval attacks [15], with no negative effects to the plants [16; 15]. However, the effects of DFMO vary in different organic systems, ranging from inhibition to stimulation of PA levels and plant development, depending on the concentration 4 [17][18][19][20], and the existence of compensatory mechanisms, such as the induction of the arginine decarboxylase (ADC) pathway [21] or an increase in the ODC protein turnover, as ODC is a short-living enzyme in plant cells [22].…”

Section: Introductionmentioning

confidence: 99%

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Polyamines may influence phytochelatin synthesis during Cd stress in rice

Pál

Csávás

Szalai

et al. 2017

Journal of Hazardous Materials

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Highlights Putrescine pre-treatment increased cadmium toxicity in rice.  In contrast, putrescine synthesis inhibition alleviated cadmium stress.  The synthesis of higher polyamines and phytochelatins is antagonistically related.  Putrescine may decrease phytochelatin synthesis at enzymatic and gene expression levels. Although the metabolism of phytochelatins and higher polyamines are linked with each other, the direct relationship between them under heavy metal stress has not yet been clarified. Two approaches were used to reveal the influence of polyamine content on cadmium stress responses, particularly with regard to phytochelatin synthesis: putrescine pre-treatment of rice 2 plants followed by cadmium stress, and treatment with the putrescine synthesis inhibitor, 2-(difluoromethyl)ornithine combined with cadmium treatment. The results indicated that putrescine pre-treatment enhanced the adverse effect of cadmium, while the application of 2-(difluoromethyl)ornithine reduced it to a certain extent. These differences were associated with increased polyamine content, more intensive polyamine metabolism, but decreased thiol and phytochelatin contents. The gene expression level and enzyme activity of phytochelatin synthase also decreased in rice treated with putrescine prior to cadmium stress, compared to cadmium treatment alone. In contrast, the inhibition of putrescine synthesis during cadmium treatment resulted in higher gene expression level of phytochelatin synthase. The results suggest that polyamines may have a substantial influence on phytochelatin synthesis at several levels under cadmium stress in rice. Abstract

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Section: Discussionmentioning

confidence: 42%

Section: Introductionmentioning

confidence: 99%

Polyamines may influence phytochelatin synthesis during Cd stress in rice

Pál

Csávás

Szalai

et al. 2017

Journal of Hazardous Materials

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“…It is probable that functioning of Spm may depend on the level of its increase upon chilling. Guye et al (1986), Nadeau et al (1987), and Lee et al (1997) have shown that Put is primarily responsible for the chilling tolerance of bean, wheat, and rice, respectively. In cucumber however, Put does not seem to play a role by itself because MGBG promoted chilling injury of cv Jinchun No.…”

Section: Discussionmentioning

confidence: 99%

“…It has been found that chilling-tolerant plants increase endogenous PA levels in response to chilling to a much greater extent than chilling-sensitive ones (Guye et al, 1986;Nadeau et al, 1987;Wang, 1989, 1990;Lee, 1997). These findings indicate, but do not prove, the involvement of PAs in chilling tolerance of plants (Bouchereau et al, 1999).…”

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confidence: 88%

Involvement of Polyamines in the Chilling Tolerance of Cucumber Cultivars

2000

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The possible involvement of polyamines (PAs) in the chilling tolerance of cucumber (Cucumis sativus L. cv Jinchun No. 3 and cv Suyo) was investigated. Plants with the first expanded leaves were exposed to 3°C or 15°C in the dark for 24 h (chilling), and then transferred to 28°C/22°C under a 12-h photoperiod for another 24 h (rewarming). Chilling-tolerant cv Jinchun No. 3 showed a marked increase of free spermidine (Spd) in leaves, once during chilling and again during rewarming. Putrescine increased significantly during rewarming, but the increase of spermine was slight. Any of these PAs did not increase in chilling-sensitive cv Suyo during either period. PA-biosynthetic enzyme activities appear to mediate these differences between cultivars. Pretreatment of Spd to cv Suyo prevented chill-induced increases in the contents of hydrogen peroxide in leaves and activities of NADPH oxidases and NADPH-dependent superoxide generation in microsomes and alleviated chilling injury. Pretreatment of methylglyoxal-bis-(guanylhydrazone), a PA biosynthesis inhibitor, to chilled cv Jinchun No. 3 prevented Spd increase and enhanced microsomal NADPH oxidase activity and chilling injury. The results suggest that Spd plays important roles in chilling tolerance of cucumber, probably through prevention of chill-induced activation of NADPH oxidases in microsomes.

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“…This has limitations such as the subjectivity of ratings. Several physiological parameters have been used to characterize the response of rice seedlings to low temperature including electrolyte leakage (Bertin et al 1996;Morsy et al 2005), antioxidant levels/lipid peroxidation (Huang and Guo 2005;Guo et al 2006;Morsy et al 2007), hormones (Lee et al 1993), polyamines (Lee et al 1995;Lee 2007) and sugars (Morsy et al 2007) although most of these studies examined relatively few rice accessions. Electrolyte leakage has been shown to be a good indicator of rice seedling chilling tolerance and its use in screening germplasm for breeding has been suggested (Bertin et al 1996).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of seedling cold tolerance in rice cultivars: a comparison of visual ratings and quantitative indicators of physiological changes

Kim

Tai

2011

Euphytica

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Rice (Oryza sativa L.) is sensitive to prolonged exposure to low temperature, which at the seedling stage can result in significant chilling injury and mortality. The objective of this study was to quantify physiological and biochemical changes in rice seedlings undergoing chilling stress and compare those changes with visual evaluation of tolerance. Seedlings from the cultivars M-202 (tolerant) and IR50 (sensitive) were subjected to 9°C for 14 days in a controlled environment chamber. Leaf tissues were harvested at various time points for determination of electrolyte leakage, proline, malondialdehyde, ascorbic acid and reduced glutathione. Significant differences between M-202 and IR50 were detected in electrolyte leakage, proline, and ascorbic acid starting at 7 days with IR50 exhibiting higher levels of these indicators. Most IR50 seedlings were dead at 14 days. A set of fifty rice accessions including M-202 and IR50 was evaluated at 10 days of cold treatment to examine the correlation of visual ratings with the physiological indices. Visual ratings were most highly correlated with electrolyte leakage and least correlated with proline content. Based on visual ratings and the physiological indices, we identified several cultivars that outperformed M-202 in cold tolerance while IR50 had the lowest tolerance of the cultivars tested.

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Polyamine regulation of growth and chilling tolerance of rice (Oryza sativa L.) roots cultured in vitro

Cited by 70 publications

References 16 publications

Polyamines may influence phytochelatin synthesis during Cd stress in rice

Polyamines may influence phytochelatin synthesis during Cd stress in rice

Involvement of Polyamines in the Chilling Tolerance of Cucumber Cultivars

Evaluation of seedling cold tolerance in rice cultivars: a comparison of visual ratings and quantitative indicators of physiological changes

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