Polyamine metabolism in primary human colon adenocarcinoma cells (SW480) and their lymph node metastatic derivatives (SW620)

Duranton, B; Holl, Vincent; Schneider, Yann; Carnesecchi, Stéphanie; Gossé, Francine; Raul, Françis; Seiler, Nikolaus

doi:10.1007/s00726-002-0333-5

Cited by 29 publications

(17 citation statements)

References 30 publications

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“…The 1,338 cm −1 band with mixed contributions of DNA and CH vibrations showed this same trend. This is consistent with the SW620 having larger RNA content [51] and nuclear area [52] that SW480.…”

Section: Distinction Between Primary and Secondary Tumour Cellssupporting

confidence: 86%

“…[53,61] This can be attributed to the Warburg effect, due to which highly proliferative cancerous cells have increased lactate contents; HCT116, SW620, and HT29 are known to have lower doubling times than SW480 and HL60 cells. [51,[62][63][64] For the carcinoma cell lines, the lactate contribution appears to be correlated with the cancer stage.…”

Section: Average and Multivariate Analysis Of Results Of Multiple Cmentioning

confidence: 99%

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Biochemical fingerprint of colorectal cancer cell lines using label‐free live single‐cell Raman spectroscopy

Pablo

Armistead

Peyman

et al. 2018

J Raman Spectroscopy

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Label‐free live single‐cell Raman spectroscopy was used to obtain a chemical fingerprint of colorectal cancer cells including the classification of the SW480 and SW620 cell line model system, derived from primary and secondary tumour cells from the same patient. High‐quality Raman spectra were acquired from hundreds of live cells, showing high reproducibility between experiments. Principal component analysis with linear discriminant analysis yielded the best cell classification, with an accuracy of 98.7 ± 0.3% (standard error) when compared with discrimination trees or support vector machines. SW480 showed higher content of the disordered secondary protein structure Amide III band, whereas SW620 showed larger α‐helix and β‐sheet band content. The SW620 cell line also displayed higher nucleic acid, phosphates, saccharide, and CH 2 content. HL60, HT29, HCT116, SW620, and SW480 live single‐cell spectra were classified using principal component analysis or linear discriminant analysis with an accuracy of 92.4 ± 0.4% (standard error), showing differences mainly in the β‐sheet content, the cytochrome C bands, the CH‐stretching regions, the lactate contributions, and the DNA content. The lipids contributions above 2,900 cm −1 and the lactate contributions at 1,785 cm −1 appeared to be dependent on the colorectal adenocarcinoma stage, the advanced stage cell lines showing lower lipid, and higher lactate content. The results demonstrate that these cell lines can be distinguished with high confidence, suggesting that Raman spectroscopy on live cells can distinguish between different disease stages, and could play an important role clinically as a diagnostic tool for cell phenotyping.

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Section: Distinction Between Primary and Secondary Tumour Cellssupporting

confidence: 86%

Section: Average and Multivariate Analysis Of Results Of Multiple Cmentioning

confidence: 99%

Biochemical fingerprint of colorectal cancer cell lines using label‐free live single‐cell Raman spectroscopy

Pablo

Armistead

Peyman

et al. 2018

J Raman Spectroscopy

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“…In fact, the less differentiated SW480 cells (from a moderately differentiated colon adenocarcinoma, grade IV Duke B) showed a more pronounced susceptibility to VK1 action due to a more marked antiproliferative effect, an increased Bax/Bcl-2 ratio persisting up to 72 h, and a significant increase in the phosphorylation of ERK 1/2 starting from low VK1 concentrations in comparison to the more differentiated HT-29 and Caco-2 cell lines. These data are in accordance with the reported high capacity of de novo polyamine biosynthesis and of polyamine uptake ascribed to this cell line, representing probably a precondition for the rapid growth ability shown by SW480 [ 35 ].…”

Section: Discussionsupporting

confidence: 92%

Vitamin K1 Exerts Antiproliferative Effects and Induces Apoptosis in Three Differently Graded Human Colon Cancer Cell Lines

Orlando

Linsalata

Tutino

et al. 2015

BioMed Research International

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Vitamin K1 has been demonstrated as having anticancer potentiality mainly in liver cancer cells. Beyond the reported mechanisms of cancer inhibition (cell cycle arrest and induction of apoptosis), a possible control by vitamin K1 on molecules affecting cell growth could be hypothesized. In the literature, few (if any) data are available on its antitumor effects on colon cancer cells. Therefore, the aims of the study were to investigate in three differently graded human colon cancer cell lines (Caco-2, HT-29, and SW480) the effects of increasing concentrations of vitamin K1 (from 10 μM to 200 μM) administered up to 72 h on (1) cell proliferation, (2) apoptosis with the possible involvement of the MAPK pathway, and (3) polyamine biosynthesis. Vitamin K1 treatment caused a significant antiproliferative effect and induced apoptosis in all the cell lines, with the involvement of the MAPK pathway. A concomitant and significant decrease in the polyamine biosynthesis occurred. This is the first study demonstrating a significant polyamine decrease in addition to the antiproliferative and proapoptotic effects following vitamin K1 administration to colon cancer cell lines. Therapeutically, combinations of vitamin K1 with polyamine inhibitors and/or analogues may represent a suitable option for chemoprevention and/or treatment in future strategies for colorectal cancer management.

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“…Moreover, this evidence indicates a different proliferative behavior and a different response to VK1 by gastric and colon cancer cells, with the latter showing a more pronounced susceptibility to VK1 action. This cell line demonstrated growth ability closely linked to the high capacity of de novo polyamine biosynthesis and polyamine uptake (48). This evidence supports the hypothesis that VK1 may have different effects on different types of cell lines.…”

Section: Discussionsupporting

confidence: 81%

Inhibitory effect of vitamin K1 on growth and polyamine biosynthesis of human gastric and colon carcinoma cell lines

Linsalata

Orlando

Tutino

et al. 2015

International Journal of Oncology

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Gastric and colon cancers remain the leading cause of cancer mortality throughout the world. Since the gastrointestinal tract works in a constant link with the external environment, chemoprevention by dietary constituents could represent a possible approach to reduce cancer risk. Dietary vitamin K1 (VK1) has been shown to prevent the growth of many types of cancer cells. However, no data are available on possible different susceptibility to VK1 by gastric or colon neoplastic cell lines. Moreover, the exact mechanism of action of VK1 is still object of investigation, even if it has been reported that VK1 may induce cell cycle arrest and apoptosis. Therefore, molecules affecting cell growth such as the natural polyamines could be of interest in VK1 action. The aim of the present study was to investigate the effects of increasing concentrations of VK1 (from 10 to 200 µM) administered up to 72 h, on the cell proliferation and apoptosis of a gastric (HGC-27) and a colon (SW480) cancer cell line. Additionally, the polyamine biosynthesis and the MAPK pathway were also examined. VK1 treatments caused an inhibition of cell proliferation and an induction of apoptosis in both cell lines, with a concomitant significant decrease of the polyamine biosynthesis, increased phospho-ERK 1/2 expression was also observed. A different proliferative behavior and a different response to VK1 by gastric and colon cancer cells was evident, with colon cells showing a more pronounced susceptibility to VK1 action. VK1 is safe and without known toxicities in adult humans, consequently it could be effective in prevention and treatment of selected gastrointestinal neoplasms. Protocols based on the use of VK1, along with polyamine inhibitors and/or analogues, could represent a suitable alternative option for improving the efficacy of chemoprevention and treatment in future strategies for gastrointestinal cancer management.

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Polyamine metabolism in primary human colon adenocarcinoma cells (SW480) and their lymph node metastatic derivatives (SW620)

Cited by 29 publications

References 30 publications

Biochemical fingerprint of colorectal cancer cell lines using label‐free live single‐cell Raman spectroscopy

Biochemical fingerprint of colorectal cancer cell lines using label‐free live single‐cell Raman spectroscopy

Vitamin K1 Exerts Antiproliferative Effects and Induces Apoptosis in Three Differently Graded Human Colon Cancer Cell Lines

Inhibitory effect of vitamin K1 on growth and polyamine biosynthesis of human gastric and colon carcinoma cell lines

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