Polyamine Metabolism in an Incompatible Interaction between Barley and the Powdery Mildew Fungus, <i>Blumeria graminis</i> f. sp. <i>hordei</i>

Cowley, Tracy; Walters, Dale R.

doi:10.1046/j.1439-0434.2002.00816.x

Cited by 35 publications

(22 citation statements)

References 25 publications

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“…The resistant genotypes had 2-3 times higher content of spermidine and spermine. Similar results were observed in an incompatible interaction between barley and powdery mildew, levels of free spermidine and of conjugated forms of putrescine and spermidine were found to increase after inoculation (Cowley and Walters 2002a). Furthermore, these results are in agreement with a previous report where the over expression of a key polyamine biosynthetic gene arginine decarboxylase (adc) in transgenic eggplant exhibited increased resistance against Fusarium wilt as compared to wild type seedlings.…”

Section: Polyamine Oxidase (Pao) Activitysupporting

confidence: 91%

Polyamine metabolism and lipoxygenase activity during Fusarium oxysporum f. sp. ricini -Castor interaction

Mhaske

Mahatma

Jha

et al. 2013

Physiol Mol Biol Plants

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Polyamine oxidase and lipoxygenase enzymes are key players for hyper sensitive reaction (HR) during incompatible interaction of host-pathogen. Thus, the role of lipoxygenase and polyamines was studied in the wilt pathogen infected and non infected tissues of resistant and susceptible genotypes of castor at 0 days after infection (DAI), 5 DAI and 10 DAI (30 days after sowing). The lipoxygenase (LOX) and polyamine oxidase (PAO) activities were higher in the incompatible interaction at all the stages of analysis. The constitutive level of malondyaldehyde (MDA) content, a product of lipid peroxidation was higher in susceptible genotypes (VP-1 and VI-9), while induced level was higher in resistant genotypes (48-1 and SKP-84) at 5 DAI and 10 DAI . Polyamine profiling using HPTLC showed higher spermidine and spermine content in resistant genotypes at 10 DAI. Furthermore, spermidine was detected only in the roots of resistant genotypes at 10 DAI. These results suggest the role of high titers of polyamines, LOX and PAO in disease resistance possibly through HR induction.

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Section: Polyamine Oxidase (Pao) Activitysupporting

confidence: 91%

Polyamine metabolism and lipoxygenase activity during Fusarium oxysporum f. sp. ricini -Castor interaction

Mhaske

Mahatma

Jha

et al. 2013

Physiol Mol Biol Plants

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“…Spot 4 was a key enzyme responsible for regulating biosynthesis of a polyamine, spermidine, in planta. Cowley and Walters [50] reported enhanced spermidine synthase activity and accumulation of spermidine in the incompatible interaction between barley and the powdery mildew fungus Bgh. Following pathogen perception by the host plant, profound changes in gene expression, DNA remodeling, and proteins (at the transcriptional and posttranslational levels) are integral steps in activating defense-responsive genes [51].…”

Section: Proteins Involved In Gene Expression and Protein Turnovermentioning

confidence: 99%

Proteomic Analysis of the Defense Response of Wheat to the Powdery Mildew Fungus, Blumeria graminis f. sp. tritici

Mandal

Zhang

et al. 2014

Protein J

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Powdery mildew of wheat is caused by Blumeria graminis f. sp. tritici (Bgt). Although many wheat cultivars resistant to this disease have been developed, little is known about their resistance mechanisms. The aim of this study was to identify proteins showing changes in abundance during the resistance response of the wheat line N0308 infected by Bgt. In two-dimensional electrophoresis analyses, 45 spots on the gels showed significant changes in abundance at 24, 48, and 72 h after inoculation, as compared to non-inoculated plants. Of these 45 proteins, 44 were identified by mass spectrometry analysis using the NCBInr database of Triticum aestivum (26 spots) and closely related species in the Triticum genus (18 spots). These proteins were associated with the defense response, photosynthesis, metabolism, and other cellular processes in wheat. Most of the up-regulated proteins were identified as stress- and defense-related proteins. In particular, the product of a specific powdery mildew resistance gene (Pm3b and its homolog) and some other defense- and pathogenesis-related proteins were overexpressed. The resistance gene product mediates the immune response and coordinates other cellular processes during the resistance response to Bgt.

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“…Ammonium provision seems to specifically activate genes involved in the hormonal signaling underlying defense responses, leading to suppose that it might positively affect the tolerance to biotic stress (Figures 3 and 4), confirming that the N form provided could potentially affect the degree of plant resistance [54][55][56].…”

Section: Discussionmentioning

confidence: 72%

Nitrate and Ammonium Affect the Overall Maize Response to Nitrogen Availability by Triggering Specific and Common Transcriptional Signatures in Roots

Ravazzolo

Trevisan

Forestan

et al. 2020

IJMS

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Nitrogen (N) is an essential macronutrient for crops. Plants have developed several responses to N fluctuations, thus optimizing the root architecture in response to N availability. Nitrate and ammonium are the main inorganic N forms taken up by plants, and act as both nutrients and signals, affecting gene expression and plant development. In this study, RNA-sequencing was applied to gain comprehensive information on the pathways underlying the response of maize root, pre-treated in an N-deprived solution, to the provision of nitrate or ammonium. The analysis of the transcriptome shows that nitrate and ammonium regulate overlapping and distinct pathways, thus leading to different responses. Ammonium activates the response to stress, while nitrate acts as a negative regulator of transmembrane transport. Both the N-source repress genes related to the cytoskeleton and reactive oxygen species detoxification. Moreover, the presence of ammonium induces the accumulation of anthocyanins, while also reducing biomass and chlorophyll and flavonoids accumulation. Furthermore, the later physiological effects of these nutrients were evaluated through the assessment of shoot and root growth, leaf pigment content and the amino acid concentrations in root and shoot, confirming the existence of common and distinct features in response to the two nitrogen forms.

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Polyamine Metabolism in an Incompatible Interaction between Barley and the Powdery Mildew Fungus, Blumeria graminis f. sp. hordei

Cited by 35 publications

References 25 publications

Polyamine metabolism and lipoxygenase activity during Fusarium oxysporum f. sp. ricini -Castor interaction

Polyamine metabolism and lipoxygenase activity during Fusarium oxysporum f. sp. ricini -Castor interaction

Proteomic Analysis of the Defense Response of Wheat to the Powdery Mildew Fungus, Blumeria graminis f. sp. tritici

Nitrate and Ammonium Affect the Overall Maize Response to Nitrogen Availability by Triggering Specific and Common Transcriptional Signatures in Roots

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