Polyamine distribution in cellular compartments of blood and in aging erythrocytes

Cooper, Kevin D.; Shukla, Jayesh B.; Rennert, Owen M.

doi:10.1016/0009-8981(76)90307-7

Cited by 74 publications

(30 citation statements)

References 20 publications

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“…In human blood, the majority of the blood polyamines are located in blood cells, such as leukocytes and erythrocytes (23,41). A previous report and our preliminary experiment showed that the polyamine concentrations in leukocytes reflect those in whole blood and in erythrocytes (41).…”

Section: Discussionmentioning

confidence: 74%

Spermine, a Natural Polyamine, Suppresses LFA-1 Expression on Human Lymphocyte

Soda¹,

Kano²,

Nakamura³

et al. 2005

The Journal of Immunology

113

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Natural polyamines, spermine, spermidine, and putrescine, play a pivotal role in the regulation of gene expression; therefore, the age-dependent decreases and the disease-dependent increases in polyamine synthesis suggest a possible contribution of polyamines to the age-related and disease-associated changes in cellular function. In this study, we examined the effects of polyamines on the cellular function and the expression of adhesion molecules on human PBMCs from healthy volunteers. Flow cytometry revealed that PBMCs cultured with spermine decreased mean fluorescent intensities (MFIs) of CD11a and CD18 in the lymphocyte light-scattered region, but not in the monocyte region. This suppression was observed in a dose- and time-dependent manner and found nonspecifically on all cell subsets we tested (CD3+, CD4+, CD8+, CD19+, CD45RA+, CD45RO+, CD4+CD45RA+, CD4+CD45RO+, CD8+CD45RA+, CD8+CD45RO+). The decreases of CD11a and CD18 MFIs were accompanied by the decrease in adherent capacity of PBMCs to HUVECs. Spermine did not hinder cell activities or cell viability. Among 42 healthy volunteers (mean, 49.5 years old; from 26 to 69), blood spermine levels inversely correlated with the CD11a MFIs of cells in the lymphocyte region (r = −0.48; p = 0.001), but not with those in the monocyte region. The effects of spermidine seemed weaker than those of spermine, and blood spermidine levels had no correlation with CD11a MFIs of the lymphocyte region. Putrescine had no effect on the expressions of membrane molecules. Polyamines, especially spermine, decrease LFA-1 (CD11a/CD18) expression on human lymphocyte and adhesion capacity of PBMCs to HUVECs.

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Section: Discussionmentioning

confidence: 74%

Spermine, a Natural Polyamine, Suppresses LFA-1 Expression on Human Lymphocyte

Soda¹,

Kano²,

Nakamura³

et al. 2005

The Journal of Immunology

113

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“…In examining polyamine levels in erythrocytes ageseparated by density, Cooper et al (1976) found that polyamine levels in old erythrocytes were significantly less than in young erythrocytes. Consequently, since most of erythrocyte components lost during aging are membrane associated, polyamines may be associated mostly with the cell membrane.…”

Section: Discussionmentioning

confidence: 98%

“…It was recently demonstrated Cooper et al, 1976;Saeki et al, 1978) that erythrocytes contain more than 80% of the spermidine and more than 7000 of the spermine in whole blood. Based on these findings, preliminary studies (Cooper et al, 1978;Saeki et al, 1978) have demonstrated that erythrocyte polyamine levels from patients with solid tumours were raised.…”

Section: Discussionmentioning

confidence: 99%

“…These samples were hydrolysed in 6N HCI for 16 h at 104°C, extracted twice with ether, and again lyophilized. The residues were dissolved in 0 5 N HCI and analysed for polyamines, using a Durrum D-500 high-pressure amino acid analyser (Russell & Russell, 1975;Cooper et al, 1976 As shown in Fig. 1, putrescine levels in both erythrocytes and plasma significantly increased as early as 1 week after inoculation, reaching the maximum level at 4 weeks.…”

Section: Methodsmentioning

confidence: 99%

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Raised polyamines in erythrocytes from melanoma-bearing mice and patients with solid tumours

Takami¹,

Nishioka²

1980

Br J Cancer

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Summary.-The levels of polyamines (putrescine, spermidine and spermine) in erythrocytes and plasma were studied using Cloudman S-91 melanoma grown in the lungs of DBA/2 mice. Polyamine levels and the numbers of tumour-cell colonies in the lungs were determined at weekly intervals. Putrescine levels in both erythrocytes and plasma significantly increased 1 week after tumour inoculation. Three weeks after inoculation, however, putrescine levels in the erythrocytes showed a greater increase than those in plasma. Spermidine and spermine levels were initially high at 2 weeks in plasma and at 4 weeks in erythrocytes. However, by 6 weeks the spermidine levels showed a greater increase in erythrocytes than in plasma. These data suggest that erythrocytes may absorb and store polyamines released into the circulation.This finding was subsequently applied to human studies. Fifty-two untreated patients with solid tumours were examined in the preoperative period. All erythrocyte polyamine levels from patients were significantly higher than those from control subjects. Plasma spermidine levels in patients were significantly higher than those in controls, whereas plasma putrescine and spermine levels showed no significant increase. The frequency of raised levels of putrescine, spermidine and spermine in erythrocytes was significantly greater than in plasma. These results suggest that polyamine levels in erythrocytes may provide useful information for the detection of cancer.

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“…The method used in this study to assess the blood cell PA content did not discriminate between erythrocytes (RBCs) and leukocytes, since analyses were performed on all blood cell types (gathered through a centrifugation step; see above). Nevertheless, as erythrocytes greatly outnumber leukocytes, the blood cell PA contents largely reflect the RBC PA content (Cooper et al 1976). …”

Section: Methodsmentioning

confidence: 99%

Pancreatic Exocrine Secretions as a Source of Luminal Polyamines in Pigs

Loret

Brolet

Pierzynowski

et al. 2000

Experimental Physiology

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The goal of the present study was twofold: (1) to detect the possible storage of dietary polyamines (PAs) in various tissues and (2) to investigate the role of dietary PAs in the differentiation of the pig intestinal epithelium. A first experimental series was designed to assess the accumulation of either milk PAs (mostly spermidine) or orally administered spermine (SPM) in piglet red blood cells (RBCs) and plasma, a preliminary stage in their distribution to growing and storage organs. Though PA concentrations of piglet RBCs and plasma were generally significantly higher than their sow counterparts, our experimental conditions failed to demonstrate that this increase could stem from ingested PAs. A second experimental series dealt with the determination of disaccharidase specific activities in proximal and distal parts of piglet gut on the 26th and 29th days after birth (preweaning time). In agreement with observations made previously on rat pups, we observed an increase in maltase specific activity (SA) at the end of the suckling period (the observed increase in sucrase SA was not significant). However, orally administered SPM did not affect this activity. Compared to the constant protein concentrations observed in both parts of the gut, the pancreatic protein content decreased sharply between the 26th and 29th postnatal days. At the same time pancreatic concentrations of spermidine (SPD) also decreased, suggesting that some pancreatic PAs were released as the organ secreted its proteins. In accordance with this hypothesis, we recorded SPM and SPD in pancreatic juice. The increases in PA concentrations seemed to follow the protein secretion pattern (i.e. PA concentrations reached a maximal value when the protein concentration was highest). The presence of PAs in pancreatic juice could be indicative of a control mechanism exerted by the pancreas on PA‐induced growth and differentiation of porcine intestinal epithelium.

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Polyamine distribution in cellular compartments of blood and in aging erythrocytes

Cited by 74 publications

References 20 publications

Spermine, a Natural Polyamine, Suppresses LFA-1 Expression on Human Lymphocyte

Spermine, a Natural Polyamine, Suppresses LFA-1 Expression on Human Lymphocyte

Raised polyamines in erythrocytes from melanoma-bearing mice and patients with solid tumours

Pancreatic Exocrine Secretions as a Source of Luminal Polyamines in Pigs

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