Polyamine and Its Metabolite H2O2 Play a Key Role in the Conversion of Embryogenic Callus into Somatic Embryos in Upland Cotton (Gossypium hirsutum L.)

Wei, Cheng; Wang, Fanlong; Cheng, Xinqi; Zhu, Qian‐Hao; Sun, Yuqiang; Zhu, Huayu; Sun, Jie

doi:10.3389/fpls.2015.01063

Cited by 40 publications

(42 citation statements)

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“…The method for cotton cultivar Xinluzao 33 SE used in this study has been previously described (Cheng et al 2015 ; Sun et al 2006 ). Non-embryogenic callus (NEC) (45 days), EC and SEs (a mixture of globular embryos, torpedo embryos and cotyledon embryos; SEs) (Supplementary Fig.…”

Section: Methodsmentioning

confidence: 99%

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De novo transcriptome analysis reveals insights into dynamic homeostasis regulation of somatic embryogenesis in upland cotton (G. hirsutum L.)

et al. 2016

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Plant regeneration via somatic embryogenesis (SE) is the key step for genetic improvement of cotton (Gossypium hirsutum L.) through genetic engineering mediated by Agrobacteria, but the molecular mechanisms underlying SE in cotton is still unclear. Here, RNA-Sequencing was used to analyze the genes expressed during SE and their expression dynamics using RNAs isolated from non-embryogenic callus (NEC), embryogenic callus (EC) and somatic embryos (SEs). A total of 101, 670 unigenes were de novo assembled. The genes differentially expressed (DEGs) amongst NEC, EC and SEs were identified, annotated and classified. More DEGs were found between SEs and EC than between EC and NEC. A significant number of DEGs were related to hormone homeostasis, stress and ROS responses, and metabolism of polyamines. To confirm the expression dynamics of selected DEGs involved in various pathways, experiments were set up to investigate the effects of hormones (Indole-3-butytric acid, IBA; Kinetin, KT), polyamines, H2O2 and stresses on SE. Our results showed that exogenous application of IBA and KT positively regulated the development of EC and SEs, and that polyamines and H2O2 promoted the conversion of EC into SEs. Furthermore, we found that low and moderate stress is beneficial for proliferation of EC and SEs formation. Together, our global analysis of transcriptomic dynamics reveals that hormone homeostasis, polyamines, and stress response synergistically regulating SE in cotton.Electronic supplementary materialThe online version of this article (doi:10.1007/s11103-016-0511-6) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

“…Suspension culture of uniform EC that was used in various physiological experiments outlined below was established following our previously described method (Cheng et al 2015 ).…”

Section: Methodsmentioning

confidence: 99%

De novo transcriptome analysis reveals insights into dynamic homeostasis regulation of somatic embryogenesis in upland cotton (G. hirsutum L.)

et al. 2016

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“…Callus of different genotypes were subcultured on medium with MSB1 (1.480 µM IBA, 0.465 µM KT), 6.843 mM glutamine and 3.784 mM asparagine to induce embryogenic callus. After one month subcultured on this medium, various callus differentiations were found among the 15 genotypes.…”

Section: Embryogenic Callus Induction and Somatic Embryo Formationmentioning

confidence: 99%

Somatic Embryogenesis and Plant Regeneration via Solid-liquid Alternating Culture in Elite Genotypes of Upland Cotton

Liu

Wang

Hua

2020

Preprint

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Background: Cotton is one of the most genotype-dependent plants for regeneration, in order to expand cotton regeneration genotypes and establish efficient regeneration system platform, Jiwu 2031 (glandless cotton), ND 58 and CAU 102, were selected for studying the highly efficient somatic embryos formation and regeneration via solid-liquid alternating culture system.Results: In present research, the MSB medium (MS salts adding B5 vitamins) containing 0.571 µM indole-3-acetic acid (IAA), 0.465 µM kinetin (KT) and 0.904 µM 2, 4-dichlorophenoxyacetic acid (2, 4-D) was effective for callus initiation of fourteen Upland cotton with ‘Corker 201’ as the control. ‘Jiwu 2031’, ‘ND 58’ and ‘CAU 102’ could form somatic embryos and regenerate fertile plants in a solid MSB medium containing 10.31 mM NH4NO3, and supplemented with 0.027 mM glycin, 2.460 µM indole-3-acetic acid (IBA), 0.930 µM KT, 3.784 mM asparagine and 6.843 mM glutamine. Under the solid-liquid alternating culture system, the non-embryonic callus was induced to form embryonic callus and the identical status somatic embryos were obtained in 42 days, and the period for plants regeneration was shortened to 90~120 days, with the higher proportion of normal plant regeneration.Conclusions: The solid-liquid alternating culture method could increase the rate of embryogenesis and shorten the period of plants regeneration of Upland cotton. This work provides the evidence that the glandless cotton is beneficial for somatic embryogenesis (SE) and plant regeneration.

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“…A participação das PAs no desenvolvimento do embrião somático foi primeiramente descrita por Montague et al (1978) em Daucus carota. Desde então, diversos trabalhos têm confirmado a importância das PAs para o desenvolvimento embrionário in vitro em diferentes sistemas (Astarita et al, 2003a;Steiner et al, 2007;Vieira et al, 2012;Santa-Catarina et al, 2013;Cheng et al, 2015;Salo et al, 2016). Em Allium cepa, a adição de Put associada com a Spd promove a indução de embriões somáticos, enquanto que a presença apenas da Spd estimula a maturação e a conversão de embriões em plantas (Martinez et al, 2000).…”

Section: Importância Das Poliaminas Na Embriogêneseunclassified

“…This could also be partially explained by an increase in AaADC expression levels, the preferential pathway for Put synthesis in zygotic embryos (de Oliveira et al, 2017). ADC genes have also been identified as predominant in Put biosynthesis at the embryogenic callus stage and early embryo differentiation in Gossypium hirsutum (Cheng et al, 2015).…”

Section: Effect Of Arg and Orn On Adc/odc Enzymatic Activity And Genementioning

confidence: 99%

Metabolismo de poliaminas na embriogênese zigótica e somática de Araucaria angustifolia (Bertol.) Kuntze.

Oliveira¹

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De maneira muito honrosa, gostaria de expressar aqui meus sentimentos em finalizar mais uma etapa da minha vida. Deixo aqui minha imensa gratidão a todos que participaram direta e indiretamente neste trabalho, tornando-o uma realidade e fazendo com que eu pudesse vivenciar essa experiência de aprendizado. Desta forma, registro meus agradecimentos: Primeiramente aos meus pais, José e Lúcia, que foram meus primeiros professores da vida. Muito tenho a agradecê-los pelos incontáveis ensinamentos sobre a vida, honestidade, dignidade, honra, dedicação e amor ao que se faz. Obrigado pela paciência de minha ausência em vários momentos importantes da vida de vocês. Amo vocês. À Professora Dra. Eny I. S. Floh, que me confiou tal trabalho. Sou imensamente grato à confiança, paciência, ensinamentos e puxões de orelhas. Obrigado pela enorme compreensão em vários momentos de dificuldade nos quais me encontrei, bem como pelas minhas falhas. Graças ao seu aceite e orientação pude evoluir, e muito, em minha formação científica. Minha gratidão será eterna. Ao Dr. André Luís Wendt dos Santos, pela enorme colaboração e participação neste trabalho. Obrigado por todos os ensinamentos que me passaste. Sua participação nas várias etapas desta tese foi essencial para a finalização da mesma. Deixo aqui minha gratidão. À Dra. Paula Maria Elbl, minha gratidão pelas valiosas contribuições metodológicas e conceituais que enriqueceram esse trabalho, pela orientação, por tudo o que sei sobre bioinformática e biologia molecular, pela grande amizade, paciência e conselhos. À técnica MSc. Amanda F. Macedo, a qual foi peça fundamental na realização deste trabalho. Agradeço imensamente sua paciência e dedicação como apoio técnico durante esses quatros anos. Seu conhecimento técnico é reconhecido neste trabalho.

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Polyamine and Its Metabolite H2O2 Play a Key Role in the Conversion of Embryogenic Callus into Somatic Embryos in Upland Cotton (Gossypium hirsutum L.)

Cited by 40 publications

References 82 publications

De novo transcriptome analysis reveals insights into dynamic homeostasis regulation of somatic embryogenesis in upland cotton (G. hirsutum L.)

De novo transcriptome analysis reveals insights into dynamic homeostasis regulation of somatic embryogenesis in upland cotton (G. hirsutum L.)

Somatic Embryogenesis and Plant Regeneration via Solid-liquid Alternating Culture in Elite Genotypes of Upland Cotton

Metabolismo de poliaminas na embriogênese zigótica e somática de Araucaria angustifolia (Bertol.) Kuntze.

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