Polyamine and intestinal properties in adult rats

Deloyer, Patricia; Dandrifosse, Guy; Bartholomeus, Catherine; Romain, Nadine; Klimek, Monique; Salmon, José; Gérard, Paul; Goessens, Guy; Eyssen, H.

doi:10.1079/bjn19960068

Cited by 11 publications

(3 citation statements)

References 22 publications

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“…Sousadias and Smith (1995) showed that spermine is more toxic than putrescine, and observed adverse effects on growth for dietary concentrations higher than 0.075%. The dietary incorporation of purified polyamines or live yeast that secrete polyamines influenced intestinal cell differentiation in developing and adult animals (Buts et al, 1994;Deloyer et al, 1996). In young animals, cell differentiation matures digestive functions of the intestine and pancreas in mammals (Peulen et al, 2000) and in fish (Péres et al, 1997).…”

Section: Discussionmentioning

confidence: 99%

Influence of dietary live yeast on European sea bass (Dicentrarchus labrax) larval development

et al. 2004

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Recent results have demonstrated that live yeast in diets improved gut maturation in sea bass larvae. In this study, we tested the absence and two levels of live yeast in sea bass larvae diets. Specimens were fed from time of mouth opening to 37 days after hatching a diet of 0%, 1.1%, or 5.7% wet weight of live yeast (Debaryomyces hansenii CBS 8339). Yeast incorporation improved survival 10%, and reduced malformed larvae. In groups fed 1.1% yeast, only 1% of larvae were malformed, compared to 14% in the control group. Final mean weight in groups fed 1.1% yeast was twice that of other groups. Activities and concentrations of mRNA trypsin and lipase were higher in the two groups fed yeast than in the control group, whereas activity and concentration of mRNA amylase were lower. This suggests that the pancreas matured faster in the two groups fed yeast. Activities of intestinal enzymes alkaline phosphatase, aminopeptidase N, and maltase in the group fed 1.1% yeast were higher than those in the two other groups, revealing earlier development of intestinal digestion. The best results were obtained with the diet containing 1.1% yeast cell biomass, corresponding approximately to 106 CFU g−1 in the diet and 1.1×104 CFU per larva 30 days after hatching. The dose-dependent effect of yeast on rearing performance could be attributed to the amount of polyamines secreted by live yeast in the gut lumen of larvae.

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Section: Discussionmentioning

confidence: 99%

Influence of dietary live yeast on European sea bass (Dicentrarchus labrax) larval development

et al. 2004

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“…In the rat the most recent findings in the field (for example, see Dufour et al 1988;Georges et al 1990;Dandrifosse et al 1991;Romain et al 1992Romain et al , 1998Wery et al 1992Wery et al , 1996aButs et al 1993Buts et al , 1994Kaouass et al 1993Kaouass et al , 1994Kaouass et al , 1996Kaouass et al , 1997aDeloyer et al 1996;Peulen et al 1997Peulen et al , 1998aLaschet et al 1998) have led to a working hypothesis explaining the effect of spermine on the maturation of the digestive tract, especially at the level of the intestine (Luk et al 1980;Dufour et al 1988;Georges et al 1990;Buts et al 1993;Dandrifosse et al 1999). In this organ two phases characterize the effect of spermine (Wery et al 1992(Wery et al , 1996aWery & Dandrifosse, 1993;Kaouass et al 1996;Peulen et al 1998b;Dandrifosse et al 1999;; desquamation of the intestinal epithelium is followed by a hormonal cascade.…”

Section: Information From Studies Performed Using Sucking Ratsmentioning

confidence: 99%

Are milk polyamines preventive agents against food allergy?

et al. 2000

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Insufficient polyamine intake could play a role in the induction of sensitization to dietary allergens. This proposal is based essentially on investigations made in sucking rats and in children. In sucking rats it has been established that oral administration of spermine can induce all the modifications occurring in the digestive tract at weaning. In the intestine events occur in two phases. The early event consists of desquamation of the epithelium resulting from an activation of apoptosis. The late event appears to involve an hormonal cascade in which adrenocorticotropic hormone, cytokines, bombesin and corticosterone are included. Observations in human subjects show that: (1) the spermine and spermidine concentrations are generally lower in infant formulas than in human breast milk. Mothers seem consistently to have relatively high or relatively low concentrations of spermine and spermidine in their milk. These individual variations may be due to diet, lifestyle or genetic background; (2) the probability of developing allergy can reach 80 % if the mean spermine concentration in the milk is lower than 2 nmol/ml milk. It is approximately 0 % if the mean spermine concentration is higher than 13 nmol/ml milk; (3) preliminary results show that the intestinal permeability to macromolecules differs in premature babies when they are fed on breast milk compared with infant formulas (J Senterre, J Rigo, G Forget, G Dandrifosse and N Romain, unpublished results). This difference does not seem to be present when powdered milk is supplemented with polyamines at the concentration found in breast milk; (4) spermine increases proliferation and differentiation of lymphocytes isolated from the tonsils of children.

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“…Polyamines may also increase the virulence potential of UPEC by modifying biofilm formation, affecting the conductivity of outer membrane porins, and/or altering the expression of secreted toxins and iron-chelating agents (51,56). Notably, polyamines are abundant within the intestinal tract (13,46) and can also be found in substantial quantities within vaginal secretions (9,34), where they may interact with and phenotypically modulate UPEC prior to entry into the urinary tract. UPEC may also encounter high levels of polyamines within the urinary tract, where urine polyamine concentrations can be elevated as a consequence of pregnancy or UTI (1,55).…”

Section: Discussionmentioning

confidence: 99%

Conditioning of Uropathogenic Escherichia coli for Enhanced Colonization of Host

2009

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While in transit within and between hosts, uropathogenic Escherichia coli (UPEC) encounters multiple stresses, including substantial levels of nitric oxide and reactive nitrogen intermediates. Here we show that UPEC, the primary cause of urinary tract infections, can be conditioned to grow at higher rates in the presence of acidified sodium nitrite (ASN), a model system used to generate nitrosative stress. When inoculated into the bladder of a mouse, ASN-conditioned UPEC bacteria are far more likely to establish an infection than nonconditioned bacteria. Microarray analysis of ASN-conditioned bacteria suggests that several NsrR-regulated genes and other stress-and polyamine-responsive factors may be partially responsible for this effect. Compared to K-12 reference strains, most UPEC isolates have increased resistance to ASN, and this resistance can be substantially enhanced by addition of the polyamine cadaverine. Nitrosative stress, as generated by ASN, can stimulate cadaverine synthesis by UPEC, and growth of UPEC in cadaverine-supplemented broth in the absence of ASN can also promote UPEC colonization of the bladder. These results suggest that UPEC interactions with polyamines or stresses such as reactive nitrogen intermediates can in effect reprogram the bacteria, enabling them to better colonize the host.The urinary tract is normally a sterile environment, and it is both hostile and poorly accessible to most microbes. However, roughly one-half of women in the United States experience a urinary tract infection (UTI) at least once in their lifetime, and one-quarter of affected women endure recurrence (22,25). More than 80% of UTIs are due to strains of uropathogenic Escherichia coli (UPEC), which are usually presumed to be part-time gut flora that have reached the urinary tract by ascension via the periurethral area (53). Transmission of UPEC among individuals occurs primarily by way of fecal-oral routes and, in some cases, may involve the ingestion of contaminated food products or sexual contact (15,23,33,40,41,57). In order to survive and disseminate, UPEC must be able to adapt to multiple environments and stresses both within and outside the host.When a UPEC infection occurs, recruitment of nitric oxide (NO)-producing neutrophils to the bladder is an important line of defense (26,48). Within hours of infection, the nitrite levels in the urine increase up to threefold, and eventually the levels of NO within the bladder are 30-to 50-fold higher than those in uninfected controls (39, 48). The high levels of NO are due in part to inducible NO synthase activity, which is upregulated within 6 h after infection (45). A role may also be played by endothelial NO synthase, which is upregulated and activated in the bladder mucosa by E. coli lipopolysaccharide (36) and by the bacteria themselves, which can produce NO with nitrite reductases under low-oxygen-tension conditions (12). NO is a precursor of a variety of reactive nitrogen intermediates (RNIs), such as peroxynitrite and nitrosothiols, which can inflict exten...

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Polyamine and intestinal properties in adult rats

Cited by 11 publications

References 22 publications

Influence of dietary live yeast on European sea bass (Dicentrarchus labrax) larval development

Influence of dietary live yeast on European sea bass (Dicentrarchus labrax) larval development

Are milk polyamines preventive agents against food allergy?

Conditioning of Uropathogenic Escherichia coli for Enhanced Colonization of Host

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