Polyamidoamine Dendrimers Decorated Multifunctional Polydopamine Nanoparticles for Targeted Chemo- and Photothermal Therapy of Liver Cancer Model

Abstract

The development of multifunctional drug delivery systems combining two or more nanoparticle-mediated therapies for efficient cancer treatment is highly desired. To face this challenge, a photothermally active polydopamine (PDA) nanoparticle-based platform was designed for the loading of chemotherapeutic drug and targeting of cancer cells. PDA spheres were first functionalized with polyamidoamine (PAMAM) dendrimers followed by the conjugation with polyethylene glycol (PEG) moieties and folic acid (FA) targeting ligand. The anticancer drug doxorubicin (DOX) was then absorbed on the particle surface. We performed the physico-chemical characterization of this versatile material and we assessed further its possible application in chemo- and photothermal therapy using liver cancer cell model. These nanoparticles exhibited high near-infrared photothermal conversion efficacy and allowed for loading of the drug, which upon release in specifically targeted cancer cells suppressed their growth. Using cell proliferation, membrane damage, apoptosis, and oxidative stress assays we demonstrated high performance of this nanosystem in cancer cell death induction, providing a novel promising approach for cancer therapy.

Replacing amine by azide: dopamine azide polymerization triggered by sodium periodate

“…39 The peak above is further confirmed in the oxygen deconvolution (see Fig. 3C), where a peak is observed at ∼530.7 eV which resembles that in the studies of Chehimi et al , 40,41 where C–O − /–C–O − groups were observed and attributed to a lower conjugation of the carbonyl groups in the sample. However, it can also be attributed to the iodine content of the sample, possibly iodanil hydroquinone.…”

“…39 The peak above is further confirmed in the oxygen deconvolution (see Fig. 3C), where a peak is observed at ∼530.7 eV which resembles that in the studies of Chehimi et al, 40,41 where vC-O − /-C-O − groups were observed and attributed to a lower con-…”

Mechanism of Anticancer Action of Novel Imidazole Platinum(II) Complex Conjugated with G2 PAMAM-OH Dendrimer in Breast Cancer Cells

“…However, similar to all metal complexes, especially platinum drugs, they have disadvantages, the most troublesome of which is low bioavailability, which results from their high hydrophobicity [ 31 ]. Previous studies have shown that this problem can be solved by using dendrimers as drug carriers [ 32 , 33 ]. In addition, as a result of combining a drug with a dendrimer, an increase in its cytotoxicity is often observed, which allows reducing its dose and, as a result, eliminating many side effects [ 34 ].…”

In vitro study of polydopamine nanoparticles as protective antioxidant agents in fibroblasts derived from ARSACS patients

“…PDNPs have known place in a large number of applications including the potential treatment of periodontal disease, 22 the treatment of inflammation, 23 the potential treatment of Parkinson's disease, 24 and as an anti-cancer platform against several forms of neoplasia. [25][26][27][28] In this work, we tested the effect of PDNPs on healthy and ARSACS patient-derived fibroblasts, in terms of protective antioxidant effect and of differential protein expression. We provided the first analysis of PDNPs on an ARSACS model, and, to the best of our knowledge, this is also the first work where PDNPs have been tested on human-derived primary cells through a combination of imaging studies and proteomic analysis.…”