Polyamidoamine Dendrimer and Oleic Acid-Functionalized Graphene as Biocompatible and Efficient Gene Delivery Vectors

Liu, Xiahui; Ma, Dongmei; Tang, Hao; Tan, Liang; Xie, Qingji; Zhang, Youyu; Ma, Ming; Yao, Shouzhuo

doi:10.1021/am500812h

Cited by 106 publications

(66 citation statements)

References 72 publications

(130 reference statements)

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“…In order to increase the cellular uptake of GBM nanoplatforms, membrane penetrating peptides or acids were added via various approaches. As an example, oleic acid, which exhibits a high affinity with the cell membrane and promotes its destabilization, was used to functionalize graphene and GO in combination with PAMAM dendrimers 61 . Additionally, the cationic cell penetrating peptide octaarginine was covalently engrafted onto GO flakes 63 doxorubicin onto GO-PEI-PEG using a MMP2-cleavable peptide linkage, consequently allowing the release of doxorubicin only in the presence of the enzyme, which is over expressed in cancer cells 41 .…”

Section: Strategies To Optimize Gbms As Gene Delivery Platformmentioning

confidence: 99%

“…Most of such studies do not surpass the proof-of-principle stage, assessing transgene expression but without therapeutic goals. They have explored a variety of functionalization strategies 37,45,46,52,56,64 , the conjugation to molecules that facilitate cell and nuclear internalization 42,61,63 as well as the combination with other nanoparticles 43 , all already discussed in the previous section, in an attempt to increase the efficiency of gene transfer. However, it is difficult to establish direct comparisons between the results achieved by these studies given the numerous factors ̶ e.g.…”

Section: Intracellular Molecular Sensingmentioning

confidence: 99%

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Graphene materials as 2D non-viral gene transfer vector platforms

2016

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Advances in genomics and gene therapy could offer solutions to many diseases that remain incurable today, however one of the critical reasons halting clinical progress is due to the difficulty in designing efficient and safe delivery vectors for the appropriate genetic cargo. Safety and largescale production concerns counter-balance the high gene transfer efficiency achieved with viral vectors, while non-viral strategies have yet to become sufficiently efficient. The extraordinary physicochemical, optical and photothermal properties of graphene-based materials (GBMs) could offer two-dimensional (2D) components for the design of nucleic acid carrier systems. We discuss here such properties and their implications for the optimization of gene delivery. While the design of such vectors is still in its infancy, we provide here an exhaustive and up-to-date analysis of the studies that have explored GBMs as gene transfer vectors, focusing on the functionalization strategies followed to improve vector performance and on the biological effects attained.

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Section: Strategies To Optimize Gbms As Gene Delivery Platformmentioning

confidence: 99%

Section: Intracellular Molecular Sensingmentioning

confidence: 99%

Graphene materials as 2D non-viral gene transfer vector platforms

2016

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“…Graphene and its analog, TMDCs, which are the two-dimensional (2D) sp 2 -bonded nanocarbon with excellent electronic, optical, and mechanical properties have been extensively studied in the past decades [31,32]. …”

Section: Introductionmentioning

confidence: 99%

A promising gene delivery system developed from PEGylated MoS2 nanosheets for gene therapy

et al. 2014

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A new class of two-dimensional (2D) nanomaterial, transition metal dichalcogenides (TMDCs) such as MoS2, MoSe2, WS2, and WSe2 which have fantastic physical and chemical properties, has drawn tremendous attention in different fields recently. Herein, we for the first time take advantage of the great potential of MoS2 with well-engineered surface as a novel type of 2D nanocarriers for gene delivery and therapy of cancer. In our system, positively charged MoS2-PEG-PEI is synthesized with lipoic acid-modified polyethylene glycol (LA-PEG) and branched polyethylenimine (PEI). The amino end of positively charged nanomaterials can bind to the negatively charged small interfering RNA (siRNA). After detection of physical and chemical characteristics of the nanomaterial, cell toxicity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Polo-like kinase 1 (PLK1) was investigated as a well-known oncogene, which was a critical regulator of cell cycle transmission at multiple levels. Through knockdown of PLK1 with siRNA carried by novel nanovector, qPCR and Western blot were used to measure the interfering efficiency; apoptosis assay was used to detect the transfection effect of PLK1. All results showed that the novel nanocarrier revealed good biocompatibility, reduced cytotoxicity, as well as high gene-carrying ability without serum interference, thus would have great potential for gene delivery and therapy.

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“…Liu et al developed a PAMAM and oleic acid functionalized GO nanocomposite and demonstrated transfection of HeLa and MG63¯broblast cells with luciferase gene at a high transfection e±ciency. 67 These composites also showed good physiological stability.…”

Section: Graphene-based Nanomaterials For Nucleic Acid Deliverymentioning

confidence: 95%

Graphene-Based Nanomaterials for Theranostic Applications

Roy

Jaiswal

2017

Rep. Adv. Phys. Sci.

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Graphene and graphene-based nanomaterials such as graphene oxide (GO), reduced graphene oxide (rGO) and graphene quantum dots (GQDs) have gained a lot of attention from diverse scienti¯c¯elds for applications in sensing, catalysis, nanoelectronics, material engineering, energy storage and biomedicine due to its unique structural, optical, electrical and mechanical properties. Graphene-based nanomaterials emerge as a novel class of nanomedicine for cancer therapy for several reasons. Firstly, its structural properties like high surface area and aromaticity enables easy loading of hydrophobic drugs. Secondly, presence of oxygen containing functional groups improve its physiological stability and also act as site for biofunctionalization. Thirdly, its optical absorption in the NIR region enable them to act as photoagents for photothermal and photodynamic therapies of cancer, both in vitro and in vivo. Finally, its intrinsic°u orescence property helps in bioimaging of cancer cells. Overall, graphene-based nanomaterials can act as agents for developing multifunctional theranostic platforms for carrying out more e±cient detection and treatment of cancers. This review provides a detailed summary of the di®erent applications of graphene-based nanomaterials in drug delivery, nucleic acid delivery, phototherapy, bioimaging and theranostics.

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Polyamidoamine Dendrimer and Oleic Acid-Functionalized Graphene as Biocompatible and Efficient Gene Delivery Vectors

Cited by 106 publications

References 72 publications

Graphene materials as 2D non-viral gene transfer vector platforms

Graphene materials as 2D non-viral gene transfer vector platforms

A promising gene delivery system developed from PEGylated MoS2 nanosheets for gene therapy

Graphene-Based Nanomaterials for Theranostic Applications

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