Polyamide supports for polypeptide synthesis

Abstract

The weak extinction coefficient (r ~100) observed for this band20•21 would indicate that the transition is probably dipole forbidden. ( 23) Queen Elizabeth II Fellow, 1973-1975

Non-PEG-Derived Polyethers as Solid Supports. 1. Synthesis, Swelling Studies, and Functionalization

“…Consequently a wide range of chemically different supports used in a range of different physical formats had to be developed to address specific performance needs. 33 and polyether crosslinked polyacylamides (PEGA) 34 exhibit excellent compatibility with hydrophilic solvents, responsible for their excellent performance in peptide synthesis. However these supports have only limited general applicability due to their lack of chemical stability (amide groups) under reaction conditions usually encountered in organic synthesis, which precludes their use in SPOS.…”

A polyamide support for solid‐phase protein sequencing

“…The cross linked t-butoxycarbonyl(BOC)-&alanyl-hexamethylenediamine-polydimethylacrylamide resin was prepared essentially as described previously [6], but on a ten-fold scale. On this larger scale, the polymer was obtained in a largely amorphous rather than beaded form.…”

“…The /3-alanine content was 0.24 mmol/g. The terminal BOC-group was removed by subjecting the resin to steps l-8 of the solid phase synthesis cycle [6] prior to activation of the resin. Dimethylacetamide was used in place of dimethylformamide [6].…”

“…A polydimethylacrylamide-based resin has recently been shown [6] to have significant advantages for solid-phase peptide synthesis over conventional polystyrene matrices. Since this resin is already functionalised with (protected) amino groups and appeared to North-Holland Publishing Company -Amsterdam possess all of the essential properties defined above, we have also investigated its use for solid-phase sequencing.…”

Novel Non-PEG Derived Polyethers as Solid Supports. 2. Solid-Phase Synthesis Studies

“…[20][21][22][23] We will discuss the synthesis of the aminofunctional SLURPS first, followed by linker conjugation, as this has not been reported before, followed by our SPPS of Leu-enkephalin.…”