Polyadenylic Acid Segment in mRNA Becomes Shorter with Age

Sheiness, Diana; Darnell, James

doi:10.1038/newbio241265a0

Cited by 297 publications

(103 citation statements)

References 29 publications

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“…Metallothionein mRNA which first appeared following induction contained a long poly(A)-tail of about 150-220 nucleotides, similar in size to most newly synthesized mRNA molecules (3). The poly(A) underwent a rapid shortening, such that by 5 hours after induction some of the poly(A) molecules reached a length of about 60 nucleotides.…”

Section: Discussionmentioning

confidence: 99%

“…Others have reported that the initial phase of poly(A)-shortening is more rapid, and also a steady state population of apparently more stable mRNA species with poly(A)-tails of 40-65 nucleotides has been found (3,4).…”

Section: Discussionmentioning

confidence: 99%

“…Newly synthesized mRNA molecules have poly(A) tails of about 200 nucleotides which are shortened rapidly to about 40-60 nucleotides (3). This observation led to the suggestion that the length of poly(A) may be regulating mRNA stability (3,4) and artificially deadenylated mRNA is less stable when injected into Xenopus oocytes (5,6). A tail of 30 adenylate residues was found to be sufficient to stabilize globin mRNA (7).…”

Section: Introductionmentioning

confidence: 99%

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An analysis of the rate of metallothionein mRNA POLY(A)-shortening using RNA blot hybridization

1985

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A progressive reduction in the size of rat metallothionein-l mRNA following induction by copper chloride or dexamethasone was demonstrated on RNA blots, and was shown to be due to shortening of the poly(A)-tail. The rate of poly(A) removal was the same in rat liver and kidney following copper chloride induction, in rat liver following dexamethasone induction, and in mouse liver following copper chloride induction. In mouse liver metallothionein-l and 2 mRNAs were shortened at the same rate.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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An analysis of the rate of metallothionein mRNA POLY(A)-shortening using RNA blot hybridization

1985

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“…The gradual decrease in length of the poly(A) sequence which has been demonstrated for HeLa mRNA [33,38] may also occur for globin mRNA. Duck globin mRNA, where the reticulocytes are less mature and it is thought the mRNA is more recently synthesised [6], has a considerably longer poly(A) sequence than rabbit or mouse globin mRNA.…”

Section: Elution From Poly( U)-sepharose With Pormamide Gradientsmentioning

confidence: 99%

“…We do not understand the differences between our results and theirs with TI RNase ; we have occasionally seen some breakdown of our poly(A) fragment after extensive TI RNase digestion but on these rare occasions have attributed it to small amounts of contaminating T, RNase. An alternative possible explanation derives from the progressive removal of the poly(A) sequence during translation [ 10,32,33]. Thus if the messenger RNA examined by Burr and Lingrel were on average from "old" reticulocytes, it might contain only a short poly(A) sequence ; mRNA from "young" reticulocytes would contain a much longer poly(A) moiety.…”

Section: Elution From Poly( U)-sepharose With Pormamide Gradientsmentioning

confidence: 99%

The Poly(adenylic acid) Sequence of Mouse Globin Messenger RNA

Mansbridge

Crossley

Lanyon

et al. 1974

European Journal of Biochemistry

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Mouse globin messenger RNA has been degraded by ribonuclease T, to give poly(adeny1ic acid) fragments of two size classes which run electrophoretically with standard RNA molecules approximately 75 and 50 nucleotides long. These contain no internal ribonucleotides other than adenosine and occur at the 3'-end of the mRNA molecule. The probable sequence to the 5'-side of the poly(A) tract is -G-C. Attempts to separate the DL and B mRNA activities by formamide gradient elution from poly(U)-Sepharose were unsuccessful.The evidence is more compatible with a post-transcriptional end-addition mechanism for t,he synthesis of the poly(adeny1ic acid) region of messenger RNA than a transcriptional mechanism.

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Essential factors in the kinetic analysis of RNA synthesis in HeLa cells

Puckett

Darnell

1977

Journal Cellular Physiology

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Further evidence of mRNA in HeLa cells with a half-life two hours or less is given. A kinetic model of RNA synthesis in HeLa cells is described in which equilibration of label occurs first into the acid soluble pool (evidence is given that this pool feeds RNA synthesis) and thence in nuclear and cytoplasmic molecules. The measured accumulation of label in nuclear and cytoplasmic poly (A) is examined with the model and parameters were found which are consistent with the quantitative transfer of nuclear poly(A) to the cytoplasm. The strengths and limitations of the model are discussed.

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Polyadenylic Acid Segment in mRNA Becomes Shorter with Age

Cited by 297 publications

References 29 publications

An analysis of the rate of metallothionein mRNA POLY(A)-shortening using RNA blot hybridization

An analysis of the rate of metallothionein mRNA POLY(A)-shortening using RNA blot hybridization

The Poly(adenylic acid) Sequence of Mouse Globin Messenger RNA

Essential factors in the kinetic analysis of RNA synthesis in HeLa cells

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