Polyadenylation site selection: linking transcription and RNA processing via a conserved carboxy-terminal domain (CTD)-interacting protein

Larochelle, Marc; Hunyadkürti, Judit; Bachand, François

doi:10.1007/s00294-016-0645-8

Cited by 23 publications

(19 citation statements)

References 37 publications

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“…2d ). Depletion of Pcf11 in S. pombe also affects mRNA production 31 , as expected of a protein essential for mRNA synthesis. To generate conditional strains for rna14 and ysh1 , we used the rapamycin-dependent anchor-away system 32 (Supplementary Fig.…”

Section: Resultsmentioning

confidence: 81%

Common mechanism of transcription termination at coding and noncoding RNA genes in fission yeast

et al. 2018

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Termination of RNA polymerase II (RNAPII) transcription is a fundamental step of gene expression that is critical for determining the borders between genes. In budding yeast, termination at protein-coding genes is initiated by the cleavage/polyadenylation machinery, whereas termination of most noncoding RNA (ncRNA) genes occurs via the Nrd1–Nab3–Sen1 (NNS) pathway. Here, we find that NNS-like transcription termination is not conserved in fission yeast. Rather, genome-wide analyses show global recruitment of mRNA 3′ end processing factors at the end of ncRNA genes, including snoRNAs and snRNAs, and that this recruitment coincides with high levels of Ser2 and Tyr1 phosphorylation on the RNAPII C-terminal domain. We also find that termination of mRNA and ncRNA transcription requires the conserved Ysh1/CPSF-73 and Dhp1/XRN2 nucleases, supporting widespread cleavage-dependent transcription termination in fission yeast. Our findings thus reveal that a common mode of transcription termination can produce functionally and structurally distinct types of polyadenylated and non-polyadenylated RNAs.

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Section: Resultsmentioning

confidence: 81%

Common mechanism of transcription termination at coding and noncoding RNA genes in fission yeast

et al. 2018

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“…5b and Supplementary Data 1), with 93% of genes that bind Pcf11 also binding Seb1. This suggests that Pcf11 and Seb1 are both required for proper Pol II termination but are likely to have distinct roles since both proteins are essential44.…”

Section: Resultsmentioning

confidence: 99%

The conserved protein Seb1 drives transcription termination by binding RNA polymerase II and nascent RNA

et al. 2017

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Termination of RNA polymerase II (Pol II) transcription is an important step in the transcription cycle, which involves the dislodgement of polymerase from DNA, leading to release of a functional transcript. Recent studies have identified the key players required for this process and showed that a common feature of these proteins is a conserved domain that interacts with the phosphorylated C-terminus of Pol II (CTD-interacting domain, CID). However, the mechanism by which transcription termination is achieved is not understood. Using genome-wide methods, here we show that the fission yeast CID-protein Seb1 is essential for termination of protein-coding and non-coding genes through interaction with S2-phosphorylated Pol II and nascent RNA. Furthermore, we present the crystal structures of the Seb1 CTD- and RNA-binding modules. Unexpectedly, the latter reveals an intertwined two-domain arrangement of a canonical RRM and second domain. These results provide important insights into the mechanism underlying eukaryotic transcription termination.

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“…This may be due to technical reasons (see above), or alternatively APA regulation may reflect an enhanced role for PCF11 in evolution. Accordingly, experiments in S. pombe revealed a role for Seb1, but not Pcf11, in the alternative generation of 3' ends on selected transcripts (Larochelle et al, 2017).…”

Section: The Role Of Pcf11 In Pas Selection and Transcriptional Termimentioning

confidence: 96%

Selective roles of vertebrate PCF11 in premature and full-length transcript termination

Kamieniarz-Gdula

Gdula

Panser

et al. 2018

Preprint

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The pervasive nature of RNA polymerase II (Pol II) transcription requires efficient termination. A key player in this process is the cleavage and polyadenylation (CPA) factor PCF11, which directly binds to the Pol II C-terminal domain and dismantles elongating Pol II from DNA in vitro. We demonstrate that PCF11-mediated termination is essential for vertebrate development. A range of genomic analyses, including: mNET-seq, 3' mRNA-seq, chromatin RNA-seq and ChIP-seq, reveals that PCF11 enhances transcription termination and stimulates early polyadenylation genome-wide. PCF11 binds preferentially between closely spaced genes, where it prevents transcriptional interference and downstream gene silencing. Notably, PCF11 is sub-stoichiometric to the CPA complex. Low levels of PCF11 are maintained by an auto-regulatory mechanism involving premature termination of its own transcript, and are important for normal development. Both in human cell culture and during zebrafish development, PCF11 selectively attenuates the expression of other transcriptional regulators by premature CPA and termination.

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Polyadenylation site selection: linking transcription and RNA processing via a conserved carboxy-terminal domain (CTD)-interacting protein

Cited by 23 publications

References 37 publications

Common mechanism of transcription termination at coding and noncoding RNA genes in fission yeast

Common mechanism of transcription termination at coding and noncoding RNA genes in fission yeast

The conserved protein Seb1 drives transcription termination by binding RNA polymerase II and nascent RNA

Selective roles of vertebrate PCF11 in premature and full-length transcript termination

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