Polyacetylenes accumulation in Ambrosia maritima hairy root and cell cultures after elicitation with methyl jasmonate

Abstract

Three lines of hairy root culture of Ambrosia maritima induced by Agrobacterium rhizogenes ATCC15834 were established. Thiarubrine A, thiarubrine A epoxide, thiarubrine A diol and their precursor pentayneene were produced by the hairy roots after elicitation with methyl jasmonate, the common signal molecule in the plant defense and development. Thiarubrine A diol was the main form detected in the medium. Maximum yield was achieved when the 13-day-old hairy root cultures were exposed to 40 lM methyl jasmonate for 72 h. Callus and cell suspension cultures were established and maintained on Murashige and Skoog medium supplied with a-naphthylacetic acid (NAA) and kinetin. When the cell suspension cultures were elicited with methyl jasmonate, pentayneene was the only polyacetylene produced. The yield of pentayneene in hairy root cultures was much higher (9.6 times) than that of cell suspension cultures.Abbreviations: DEPT -distortionless enhancement by polarization transfer; FAB-MS -fast atom bombardment mass spectroscopy; HMBC -heteronuclear multiple bond correlation; MeJ -methyl jasmonate

Enhanced production of antitumour naphthoquinones in transgenic hairy root lines of Lithospermum canescens

“…However, cell suspension cultures of L. erythrorhizon in M9 medium with addition of methyl jasmonate (50 lM) showed the content of ACS nearly two times higher than in the control (Gaisser and Heide 1996). The results of this study are in opposite to other investigations where methyl jasmonane has been found a very potent agent to enhance secondary metabolites production in hairy root cultures (Frankfater et al 2009;Kim et al 2009;Kittipongpatana et al 2002;Li et al 2005;Zid and Orihara 2005).…”

Biosynthesis and function of polyacetylenes and allied natural products

“…For hairy root cultures of Ambrosia maritima, the production of thiarubrine A (11Dj) and two derivatives was increased by methyl jasmonate [279]. In the presence of methyl jasmonate, cell suspension cultures only produced the biosynthetic precursor pentaynene 11E and at a level 9.6-fold lower than hairy roots.…”

Enhancement of plant performance and biochemical constituents in field and hairy root production of Echinacea

“…parthenemium roots (Stojakowska et al, 2002), and polyacetylenes were accumulated 72 h after treatment in Ambrosia maritima hairy root cultures (Zid and Orihara, 2005), which is similar to the time required to increase production of alkamides in our hairy root cultures. Similar results were obtained in Beta vulgaris, where high levels of MJ resulted in higher betalaine content after two weeks of culture (Suresh et al, 2004).…”

“…These results suggest that cultures should be challenged with the elicitor JA, when high availability of nutrients in the medium can be used for secondary metabolite biosynthesis (Zid and Orihara, 2005).…”

“…Both JA and MJ are known to be involved in plant defense response by altering gene expression (Zid and Orihara, 2005) and have been used as elicitors and as signal molecules. Jasmonic acid has been shown to increase production of secondary metabolites in at least 37 species (Gundlach et al, 1992).…”