Polyacetylene From Dendropanax morbifera Alleviates Diet-Induced Obesity and Hepatic Steatosis by Activating AMPK Signaling Pathway

Abstract

The extract tea of Dendropanax morbifera is popular beverages in Korea, and their preventive and therapeutic roles in metabolic disorders have been reported. However, the molecular mechanism has not been studied despite the known efficacy of D. morbifera. Eleven fractions (fr.1–fr.11) were divided by MPLC to find the active compound. Among them, Fr.5 was superior to others in that the inhibitory efficacy of de novo triglyceride (TG) biosynthesis. NMR analysis revealed that Fr.5 is composed 98% or more (9Z,16S)-16-hydroxy-9,17-octadecadiene-12,14-diynoic acid (HOD). Treatment of HOD diminished oleic acid (OA)-induced TG accumulation in HepG2 hepatocytes and differentiation of 3T3-L1 preadipocytes by activating LKB1/AMPK. In addition, we determined the effect of the oral administration of the extract of D. morbifera on obesity and hepatic steatosis in high-fat diet (HFD)-induced obese mice. This study proved that D. morbifera containing HOD, the active substance, can show preventive or therapeutic efficacy on obesity and hepatic steatosis through the targeting LKB1/AMPK axis.

Anti-Melanogenic Effect of Dendropanax morbiferus and Its Active Components via Protein Kinase A/Cyclic Adenosine Monophosphate-Responsive Binding Protein- and p38 Mitogen-Activated Protein Kinase-Mediated Microphthalmia−Associated Transcription Factor Downregulation

“…DMW-1 and 2 are polyacetylenebased compounds with similar structures (Figure 3), and their structural explanations are provided in the supplementary data ( Supplementary Figures S2 and S3 and Table S1). Of all polyacetylene-based compounds, several studies have reported that (9Z,16S)-16-hydroxy-9,17-octadecadiene-12,14-diynoic acid has anti-obesity, anti-osteoclastogenic, antioxidant, antiinflammatory, and anticomplement activities (Park et al, 2004;Kang et al, 2018;Kim et al, 2018a). The beneficial effect of (2Z, 8Z)-matricaria acid methyl ester has been reported to inhibit tyrosinase activity (Luo et al, 2009).…”

Dendropanax morbifera Leaf Polyphenolic Compounds: Optimal Extraction Using the Response Surface Method and Their Protective Effects against Alcohol-Induced Liver Damage

“…Specifically, Dendropanax morbifera is a subtropical evergreen tree species that is also distributed in other regions of North-East Asia, such as China and Japan. Previous studies have shown that D. morbifera leaf extracts (DMLE) are enriched with polyphenolic compounds that offer a diverse array of functionalities, including antioxidant, anti-inflammatory, anti-obesity, and anti-cancer activities, as well as immunoregulatory functions [8][9][10][11].…”

Protective Effects of Dendropanax morbifera against Cisplatin-Induced Nephrotoxicity without Altering Chemotherapeutic Efficacy

“…Previous studies indicated that D . morbifera extract was effective in attenuating the inflammatory response [19,20,21]. As demonstrated in Figure 6, levels of pro-inflammatory cytokines such as TNF-α, IL-1β, and IL-6 were significantly increased in the CDDP-treated group, with a 1.5-fold induction in the levels of these cytokines.…”