Poly-ε-Caprolactone and Polyvinyl Alcohol Electrospun Wound Dressings: Adhesion Properties and Wound Management of Skin Defects in Rabbits

Buzgo, Matěj; Plencner, Martin; Rampichová, Michala; Litvinec, Andrej; Prosecká, Eva; Staffa, Andrea; Kralovič, Martin; Filová, Eva; Doupnik, Miroslav; Lukášová, Věra; Vocetková, Karolína; Anderova, Jana; Kubíková, Tereza; Zajíček, Robert; Lopot, František; Jelen, Karel; Tonar, Zbyněk; Amler, Evžen; Divín, Radek; Fiori, F.

doi:10.2217/rme-2018-0072

Cited by 15 publications

(12 citation statements)

References 51 publications

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“…Yet, a combination of PCL, gentamicin, and VitE in a clean, non-infected wound did not worsen the healing process. These results agree with our study of an acute skin defect treatment, where PCL nanofibers showed worse results than hydrophilic polyvinyl alcohol nanofibers [ 52 ]. A similar treatment in a porcine model of the chronic wound is currently being performed to confirm these results.…”

Section: Discussionsupporting

confidence: 92%

The Preparation and Biological Testing of Novel Wound Dressings with an Encapsulated Antibacterial and Antioxidant Substance

et al. 2022

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Chronic wounds represent a significant socio-economic problem, and the improvement of their healing is therefore an essential issue. This paper describes the preparation and biological properties of a novel functionalized nanofiber wound dressing consisting of a polycaprolactone nanofiber carrier modified by a drug delivery system, based on the lipid particles formed by 1-tetradecanol and encapsulated gentamicin and tocopherol acetate. The cytotoxicity of extracts was tested using a metabolic activity assay, and the antibacterial properties of the extracts were tested in vitro on the bacterial strains Staphylococcus aureus and Pseudomonas aeruginosa. The effect of the wound dressing on chronic wound healing was subsequently tested using a mouse model. Fourteen days after surgery, the groups treated by the examined wound cover showed a lower granulation, reepithelization, and inflammation score compared to both the uninfected groups, a lower dermis organization compared to the control, a higher scar thickness compared to the other groups, and a higher thickness of hypodermis and bacteria score compared to both the uninfected groups. This work demonstrates the basic parameters of the safety (biocompatibility) and performance (effect on healing) of the dressing as a medical device and indicates the feasibility of the concept of its preparation in outpatient conditions using a suitable functionalization device.

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Section: Discussionsupporting

confidence: 92%

The Preparation and Biological Testing of Novel Wound Dressings with an Encapsulated Antibacterial and Antioxidant Substance

et al. 2022

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“…Furthermore, the samples contained a minimum of spherical defects. The stereology of the scaffolds was in accordance with other findings [14,15]. The scanning electron microscope also showed a fine fibrin network formed on the surface of the plateletfunctionalized samples.…”

Section: Discussionsupporting

confidence: 88%

Functionalization of Polymeric Nanofibers Using Platelets for Melanocyte Culture

et al. 2020

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Tissue engineering is an interdisciplinary field that uses a combination of cells, suitable biomaterials and bioactive molecules to engineer the desired tissue and restore lost function. These principles have quickly begun to spread to the therapy of multiple diseases, including depigmentation disorders. The most common depigmentation disorder is vitiligo, a disease with deep psychosocial implications. Thanks to their unique properties, electrospun polymeric nanofibers represent a material suitable for tissue engineering applications. Furthermore, they may be functionalized with platelets, cells that contain a wide spectrum of growth factors and chemokines. The aim of this paper was to evaluate the functionalization of polymeric nanofibers with platelets and their effects in melanocyte culture. The scaffolds were visualized using scanning electron microscopy, the metabolic activity and proliferation of melanocytes was determined using MTS assay and dsDNA quantification, respectively. Furthermore, the melanocytes were stained and visualized using confocal microscopy. The acquired data showed that poly-ε-caprolactone functionalized with platelets promoted the viability and proliferation of melanocytes. According to the results, such a functionalized scaffold combining nanofibers and platelets may be suitable for melanocyte culture.

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“…9 Besides, NPs promoted the wound recovery by boosting immunity, stimulating skin recovery, and killing bacteria. 10,11 For wound dressings, various materials have been used to protect the area around the wound, thereby promoting wound healing. 12 Different wound-healing methods exist in the literature, including allografts, autografts, and wound dressings, derived from biodegradable and biocompatible polymers, such as chitosan, collagen, and so on.…”

Section: Introductionmentioning

confidence: 99%

“…For example, antigen-conjugated nanoparticles (NPs) have been proven to protect the wound from bacteria infection and to promote skin recovery and regeneration . Besides, NPs promoted the wound recovery by boosting immunity, stimulating skin recovery, and killing bacteria. , For wound dressings, various materials have been used to protect the area around the wound, thereby promoting wound healing. − Different wound-healing methods exist in the literature, including allografts, autografts, and wound dressings, derived from biodegradable and biocompatible polymers, such as chitosan, collagen, and so on. − This has led to the development of materials such as a traditional membrane, dressing material decorated with NPs and biological groups to improve the wound-healing efficiency. − …”

Section: Introductionmentioning

confidence: 99%

Gold-Nanorod-Based Scaffolds for Wound-Healing Applications

Nanda

Wang

Hossain

et al. 2022

ACS Appl. Nano Mater.

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Controlling local and systemic factors during the wound-healing process, including inflammation, proliferation, and maturation, can play a key role in effective wound healing. It is worth taking advantage of matrix- or scaffold-based therapeutic approaches. Herein, a gold nanorod (GNR)-incorporated poly(lactic-co-glycolic acid) (PLGA)/poly(caprolactone) (PCL) scaffold was developed to improve the wound-healing effect by controlling heat shock protein (HSP70) via external light stimulation. The GNR-incorporated scaffold showed no harmful effects on the cells and could stimulate cell proliferation in vitro by generating mild heat generation in a timely manner with laser irradiation. A GNR-incorporated scaffold attached to the wound of mice effectively increased the local temperature to 40 °C after laser irradiation, more effectively promoting HSP70 expression and the wound-healing process compared to that of conventional dressing- and scaffold-treated mice. The GNR-incorporated scaffold and timely control HSP70 expression approach can be used as a promising wound-healing strategy for improving the therapeutic effect.

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Poly-ε-Caprolactone and Polyvinyl Alcohol Electrospun Wound Dressings: Adhesion Properties and Wound Management of Skin Defects in Rabbits

Cited by 15 publications

References 51 publications

The Preparation and Biological Testing of Novel Wound Dressings with an Encapsulated Antibacterial and Antioxidant Substance

The Preparation and Biological Testing of Novel Wound Dressings with an Encapsulated Antibacterial and Antioxidant Substance

Functionalization of Polymeric Nanofibers Using Platelets for Melanocyte Culture

Gold-Nanorod-Based Scaffolds for Wound-Healing Applications

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