Poly-γ-glutamic acid production by Bacillus subtilis 168 using glucose as the sole carbon source: A metabolomic analysis

Halmschlag, Birthe; Putri, Sastia Prama; Fukusaki, Eiichiro; Blank, Lars M.

doi:10.1016/j.jbiosc.2020.04.011

Cited by 29 publications

(16 citation statements)

References 48 publications

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“…In B . subtilis and Vibrio natriegens , citric acid cycle intermediates determine the production of the biotechnologically important storage polymers poly‐γ‐glutamic acid and poly‐β‐hydroxybutyrate, respectively (Dalia et al , 2017; Halmschlag et al , 2020). Therefore, we tested whether VcdP from V. cholerae could also increase GltA activity in these two organisms.…”

Section: Discussionmentioning

confidence: 99%

A dual‐function RNA balances carbon uptake and central metabolism in Vibrio cholerae

et al. 2021

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Bacterial small RNAs (sRNAs) are well known to modulate gene expression by base pairing with trans‐encoded transcripts and are typically non‐coding. However, several sRNAs have been reported to also contain an open reading frame and thus are considered dual‐function RNAs. In this study, we discovered a dual‐function RNA from Vibrio cholerae, called VcdRP, harboring a 29 amino acid small protein (VcdP), as well as a base‐pairing sequence. Using a forward genetic screen, we identified VcdRP as a repressor of cholera toxin production and link this phenotype to the inhibition of carbon transport by the base‐pairing segment of the regulator. By contrast, we demonstrate that the VcdP small protein acts downstream of carbon transport by binding to citrate synthase (GltA), the first enzyme of the citric acid cycle. Interaction of VcdP with GltA results in increased enzyme activity and together VcdR and VcdP reroute carbon metabolism. We further show that transcription of vcdRP is repressed by CRP allowing us to provide a model in which VcdRP employs two different molecular mechanisms to synchronize central metabolism in V. cholerae.

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Section: Discussionmentioning

confidence: 99%

A dual‐function RNA balances carbon uptake and central metabolism in Vibrio cholerae

et al. 2021

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“…2a). Carbon source metabolism at the γ-PGA level is related to several stress response proteins, such as catabolite control protein A (CcpA) (Halmschlag et al 2020). Fructose bisphosphate and excess glucose activate CcpA (Jault et al 2000).…”

Section: Optimization Of Nutritional and Culture Parameters For γ-Pga...mentioning

confidence: 99%

Enhanced poly-γ-glutamic acid production by a newly isolated Bacillus tequilensis BL01

Wang

Zhou

Gao

et al. 2022

Preprint

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Poly γ-glutamic acid (γ-PGA) is a promising biopolymer for various applications. In this study, we isolated a novel γ-PGA-producing strain, Bacillus tequilensis BL01. The one-factor-at-a-time method was used to investigate the influence of carbon and nitrogen sources and temperature on γ-PGA production. The optimal carbon and nitrogen sources were sucrose and (NH4)2SO4, respectively. The optimal temperature for γ-PGA production was determined to be 37°C. Response surface methodology was used to determine the optimum medium components: 68.9 g/L sucrose, 47.7 g/L monosodium glutamate, and 2.5 g/L K2HPO4. γ-PGA titer increased significantly from 8.6 g/L to 21.1 g/L when strain BL01 was cultivated in the optimized medium. Furthermore, γ-PGA titer reached 54.7 g/L with a productivity of 1.37 g/L/h and a yield of 2.47 g of γ-PGA/g of L-glutamic acid with the optimized medium in fed-batch fermentation. It should be noted that the γ-PGA yield in this study was the highest of all reported studies, indicating a great potential for the industrial production of γ-PGA.

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“…Using the constitutive P veg promoter, the yield of c-PGA increased by 129% when using glucose as the sole carbon source. 44 In the case of the maltose-inducible P glv promoter, which was used to secrete T9W, a variant of pig myeloid antimicrobial 36 (PMAP 36) with high antimicrobial activity, this system proved to be an economical and environmentally correct approach for biotechnological production. 47 Furthermore, maltose was likewise the inducer in a safe method to produce Cecropin AD (CAD), a hybrid peptide with biological activity.…”

Section: The Dual Promoters In B Subtilismentioning

confidence: 99%

“…Using the constitutive P veg promoter, the yield of γ-PGA increased by 129% when using glucose as the sole carbon source. 44 …”

Section: The Inducer-dependent Promoters In B Subtilismentioning

confidence: 99%

The multifunctionality of expression systems in Bacillus subtilis: Emerging devices for the production of recombinant proteins

Souza

Guimarães

Pereira

et al. 2021

Exp Biol Med (Maywood)

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Bacillus subtilis is a successful host for producing recombinant proteins. Its GRAS (generally recognized as safe) status and its remarkable innate ability to absorb and incorporate exogenous DNA into its genome make this organism an ideal platform for the heterologous expression of bioactive substances. The factors that corroborate its value can be attributed to the scientific knowledge obtained from decades of study regarding its biology that has fostered the development of several genetic engineering strategies, such as the use of different plasmids, engineering of constitutive or double promoters, chemical inducers, systems of self-inducing expression with or without a secretion system that uses a signal peptide, and so on. Tools that enrich the technological arsenal of this expression platform improve the efficiency and reduce the costs of production of proteins of biotechnological importance. Therefore, this review aims to highlight the major advances involving recombinant expression systems developed in B. subtilis, thus sustaining the generation of knowledge and its application in future research. It was verified that this bacterium is a model in constant demand and studies of the expression of recombinant proteins on a large scale are increasing in number. As such, it represents a powerful bacterial host for academic research and industrial purposes.

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Poly-γ-glutamic acid production by Bacillus subtilis 168 using glucose as the sole carbon source: A metabolomic analysis

Cited by 29 publications

References 48 publications

A dual‐function RNA balances carbon uptake and central metabolism in Vibrio cholerae

A dual‐function RNA balances carbon uptake and central metabolism in Vibrio cholerae

Enhanced poly-γ-glutamic acid production by a newly isolated Bacillus tequilensis BL01

The multifunctionality of expression systems in Bacillus subtilis: Emerging devices for the production of recombinant proteins

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