Poly-γ-glutamic acid produced from<i>Bacillus licheniformis</i>CGMCC 2876 as a potential substitute for polyacrylamide in the sugarcane industry

Yan, Shan; Yao, Haosheng; Chen, Zhen; Zeng, Shengquan; Xi, Xi; Wang, Qianqian; He, Ning; Li, Qingbiao

doi:10.1002/btpr.2118

Cited by 8 publications

(5 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As illustrated in Fig. 3 a, glycerol metabolism was markedly inhibited by the addition of glucose, while glycerol was used as a preferred carbon source in γ-PGA synthesis, which was consistent with the reports of some other studies [ 6 , 15 , 28 , 29 ].…”

Section: Resultssupporting

confidence: 90%

“…In our previous studies, B . licheniformis CGMCC2876 was observed to produce extracellular polysaccharides when using glucose as the sole carbon source [ 13 , 14 ], whereas poly-γ-glutamic acid (γ-PGA) was secreted when trisodium citrate and glycerol were used as the carbon sources [ 15 ]. Both of the extracellular polymeric substances exhibited high flocculating activities.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of glucose on poly-γ-glutamic acid metabolism in Bacillus licheniformis

Chen

et al. 2017

Microb Cell Fact

Self Cite

View full text Add to dashboard Cite

BackgroundPoly-gamma-glutamic acid (γ-PGA) is a promising macromolecule with potential as a replacement for chemosynthetic polymers. γ-PGA can be produced by many microorganisms, including Bacillus species. Bacillus licheniformis CGMCC2876 secretes γ-PGA when using glycerol and trisodium citrate as its optimal carbon sources and secretes polysaccharides when using glucose as the sole carbon source. To better understand the metabolic mechanism underlying the secretion of polymeric substances, SWATH was applied to investigate the effect of glucose on the production of polysaccharides and γ-PGA at the proteome level.ResultsThe addition of glucose at 5 or 10 g/L of glucose decreased the γ-PGA concentration by 31.54 or 61.62%, respectively, whereas the polysaccharide concentration increased from 5.2 to 43.47%. Several proteins playing related roles in γ-PGA and polysaccharide synthesis were identified using the SWATH acquisition LC–MS/MS method. CcpA and CcpN co-enhanced glycolysis and suppressed carbon flux into the TCA cycle, consequently slowing glutamic acid synthesis. On the other hand, CcpN cut off the carbon flux from glycerol metabolism and further reduced γ-PGA production. CcpA activated a series of operons (glm and epsA-O) to reallocate the carbon flux to polysaccharide synthesis when glucose was present. The production of γ-PGA was influenced by NrgB, which converted the major nitrogen metabolic flux between NH4 + and glutamate.ConclusionThe mechanism by which B. licheniformis regulates two macromolecules was proposed for the first time in this paper. This genetic information will facilitate the engineering of bacteria for practicable strategies for the fermentation of γ-PGA and polysaccharides for diverse applications.Electronic supplementary materialThe online version of this article (doi:10.1186/s12934-017-0642-8) contains supplementary material, which is available to authorized users.

show abstract

Section: Resultssupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Effect of glucose on poly-γ-glutamic acid metabolism in Bacillus licheniformis

Chen

et al. 2017

Microb Cell Fact

Self Cite

View full text Add to dashboard Cite

show abstract

“…More importantly, γ-PGA can be released into the environment to sequester toxic metal ions, decrease salt concentration [ 4 ], provide a carbon source [ 15 ], and protect against adverse conditions [ 16 ]. γ-PGA can also improve the formation of biofilms and assist absorption of essential nutrients from the environment [ 17 ].…”

Section: Overview Of γ-Pgamentioning

confidence: 99%

Microbial synthesis of poly-γ-glutamic acid: current progress, challenges, and future perspectives

Luo

Guo

Liu

et al. 2016

Biotechnol Biofuels

221

202

View full text Add to dashboard Cite

Poly-γ-glutamic acid (γ-PGA) is a naturally occurring biopolymer made from repeating units of l-glutamic acid, d-glutamic acid, or both. Since some bacteria are capable of vigorous γ-PGA biosynthesis from renewable biomass, γ-PGA is considered a promising bio-based chemical and is already widely used in the food, medical, and wastewater industries due to its biodegradable, non-toxic, and non-immunogenic properties. In this review, we consider the properties, biosynthetic pathway, production strategies, and applications of γ-PGA. Microbial biosynthesis of γ-PGA and the molecular mechanisms regulating production are covered in particular detail. Genetic engineering and optimization of the growth medium, process control, and downstream processing have proved to be effective strategies for lowering the cost of production, as well as manipulating the molecular mass and conformational/enantiomeric properties that facilitate screening of competitive γ-PGA producers. Finally, future prospects of microbial γ-PGA production are discussed in light of recent progress, challenges, and trends in this field.Electronic supplementary materialThe online version of this article (doi:10.1186/s13068-016-0537-7) contains supplementary material, which is available to authorized users.

show abstract

“…By using the same strain, Yan et al demonstrated the potential of the bio-flocculant activity of γ-PGA to replace polyacrylamide from the sugarcane industry for clarification. The presence of metal ions such as Mg 2+ could enhance the flocculating ability and γ-PGA production, unlike B. subtilis NX-2, which requires Mn 2+ ions [33]. Bajaj et al reported the ability of metabolic precursors to improve the molecular size and yield of γ-PGA by B. licheniformis NCIM 2324.…”

Section: Bacillus Licheniformismentioning

confidence: 99%

Poly-gamma-glutamic acid biopolymer: a sleeping giant with diverse applications and unique opportunities for commercialization

Nair

Navale

Dharne

2021

Biomass Conv. Bioref.

View full text Add to dashboard Cite

Poly-gamma-glutamic acid (γ-PGA) is a biodegradable, non-toxic, ecofriendly, and non-immunogenic biopolymer. Its phenomenal properties have gained immense attention in the field of regenerative medicine, the food industry, wastewater treatment, and even in 3D printing bio-ink. The γ-PGA has the potential to replace synthetic non-degradable counterparts, but the main obstacle is the high production cost and lower productivity. Extensive research has been carried out to reduce the production cost by using different waste; however, it is unable to match the commercialization needs. This review focuses on the biosynthetic mechanism of γ-PGA, its production using the synthetic medium as well as different wastes by L-glutamic acid-dependent and independent microbial strains. Furthermore, various metabolic engineering strategies and the recovery processes for γ-PGA and their possible applications are discussed. Finally, highlights on the challenges and unique approaches to reduce the production cost and to increase the productivity for commercialization of γ-PGA are also summarized.

show abstract

Poly-γ-glutamic acid produced fromBacillus licheniformisCGMCC 2876 as a potential substitute for polyacrylamide in the sugarcane industry

Cited by 8 publications

References 38 publications

Effect of glucose on poly-γ-glutamic acid metabolism in Bacillus licheniformis

Effect of glucose on poly-γ-glutamic acid metabolism in Bacillus licheniformis

Microbial synthesis of poly-γ-glutamic acid: current progress, challenges, and future perspectives

Poly-gamma-glutamic acid biopolymer: a sleeping giant with diverse applications and unique opportunities for commercialization

Contact Info

Product

Resources

About