Poly-β-hydroxybutyrate (PHB) in bioflocs alters intestinal microbial community structure, immune-related gene expression and early Cyprinid herpesvirus 2 replication in gibel carp (Carassius auratus gibelio)

Qiao, Guo; Chen, Peng; Sun, Qinqin; Zhang, Mingming; Zhang, Jialin; Li, Zhen; Li, Qiang

doi:10.1016/j.fsi.2019.12.045

Cited by 39 publications

(33 citation statements)

References 54 publications

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“…In agreement with the present study the oral administration of the OA poly-β-hydroxybutyrate (PHB) have been found to increase Bacillus sp. in gibel carp (Carassius gibelio) 72 and soiny mullet (Liza haematocheila) 73 . The authors suggested that dietary PHB supplementation changed the intestinal microbiota structure, increased probiotics growth and abundance, and inhibited opportunistic bacterial pathogen growth.…”

Section: Discussionmentioning

confidence: 99%

Effects of dietary organic acids and nature identical compounds on growth, immune parameters and gut microbiota of European sea bass

Busti

Rossi²,

Volpe

et al. 2020

Sci Rep

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A 71-day study was conducted to explore the effect of increasing dietary levels (0, 250, 500, 1000 mg kg feed−1; D0, D250, D500 and D1000, respectively) of a blend of microencapsulated organic acids (OA, specifically citric and sorbic acid) and nature identical compounds (NIC, specifically thymol and vanillin), on growth, intestinal immune parameters and gut microbiota (GM) of European sea bass juveniles reared under normal and subsequently suboptimal environmental conditions (high temperature, 30.0 ± 0.4 °C and low oxygen, 4.6 ± 0.6 mg L−1). OA and NIC did not promote growth, feed utilisation and feed intake at the inclusion tested but induced a significantly upregulation of IL-8, IL-10 and TGFβ. GM analyzed by next-generation sequencing showed that OA and NIC were able to exert prebiotic properties stimulating the development of beneficial bacteria taxa such as Lactobacillus, Leuconostoc, and Bacillus sp. Picrust analyses displayed a significant potential functional reconfiguration of GM promoting a decrease in inflammation-promoting and homeostatic functions at increasing OA and NIC administration. For the first time on this species the exposure to suboptimal rearing conditions was able to modify GM structure reducing LAB and increasing Proteobacteria, findings which were consistent with the inflammatory process observed at mRNA level.

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Section: Discussionmentioning

confidence: 99%

Effects of dietary organic acids and nature identical compounds on growth, immune parameters and gut microbiota of European sea bass

Busti

Rossi²,

Volpe

et al. 2020

Sci Rep

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“…The predictive function of the gut microbiome of fish has only been investigated in a small handful of studies (Lyons et al, 2017;Wei et al, 2018;Yang et al, 2019;Piazzon et al, 2019;Qiao et al, 2020;Piazzon et al, 2020) and to our knowledge this is one of the first investigations of gilthead sea bream. The absence of significant effects of diet or gut section indicate that replacing FO with CO did not drastically alter the composition and function of microbes in the gut.…”

Section: Discussionmentioning

confidence: 99%

Effect of dietary oil fromCamelina sativaon the growth performance, fillet fatty acid profile and gut microbiome of gilthead Sea bream (Sparus aurata)

Huyben

Rimoldi

Ceccotti

et al. 2020

PeerJ

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Background In the last two decades, research has focused on testing cheaper and sustainable alternatives to fish oil (FO), such as vegetable oils (VO), in aquafeeds. However, FO cannot be entirely replaced by VOs due to their lack of omega-3 (n-3) long-chain polyunsaturated fatty acids (LC-PUFA), particularly eicosapentaenoic (EPA; 20:5n-3) and docosahexaenoic (DHA; 22:6n-3) acids. The oilseed plant, Camelina sativa, may have a higher potential to replace FO since it can contains up to 40% of the omega-3 precursors α-linolenic acid (ALA; 18:3n-3) and linoleic acid (LA; 18:2n-6). Methods A 90-day feeding trial was conducted with 600 gilthead sea bream (Sparus aurata) of 32.92 ± 0.31 g mean initial weight fed three diets that replaced 20%, 40% and 60% of FO with CO and a control diet of FO. Fish were distributed into triplicate tanks per diet and with 50 fish each in a flow-through open marine system. Growth performance and fatty acid profiles of the fillet were analysed. The Illumina MiSeq platform for sequencing of 16S rRNA gene and Mothur pipeline were used to identify bacteria in the faeces, gut mucosa and diets in addition to metagenomic analysis by PICRUSt. Results and Conclusions The feed conversion rate and specific growth rate were not affected by diet, although final weight was significantly lower for fish fed the 60% CO diet. Reduced final weight was attributed to lower levels of EPA and DHA in the CO ingredient. The lipid profile of fillets were similar between the dietary groups in regards to total saturated, monounsaturated, PUFA (n-3 and n-6), and the ratio of n-3/n-6. Levels of EPA and DHA in the fillet reflected the progressive replacement of FO by CO in the diet and the EPA was significantly lower in fish fed the 60% CO diet, while ALA was increased. Alpha and beta-diversities of gut bacteria in both the faeces and mucosa were not affected by any dietary treatment, although a few indicator bacteria, such as Corynebacterium and Rhodospirillales, were associated with the 60% CO diet. However, lower abundance of lactic acid bacteria, specifically Lactobacillus, in the gut of fish fed the 60% CO diet may indicate a potential negative effect on gut microbiota. PICRUSt analysis revealed similar predictive functions of bacteria in the faeces and mucosa, although a higher abundance of Corynebacterium in the mucosa of fish fed 60% CO diet increased the KEGG pathway of fatty acid synthesis and may act to compensate for the lack of fatty acids in the diet. In summary, this study demonstrated that up to 40% of FO can be replaced with CO without negative effects on growth performance, fillet composition and gut microbiota of gilthead sea bream.

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“…Biofloc technology system is a shining star of aquaculture production system, which provides a lot of benefits such as improves water quality, immunity, growth and survival of culture species, and in other side, it minimizes the water and feed input and effluent production (Kumar et al., 2018). In general, biofloc system encompasses more bacterial communities compared with clear water aquaculture system; however, bacterial community of BFT varies according to the carbon source, C/N ratio, stocking density and floc characteristics (Deng et al., 2018, 2019; Huang et al., 2020; Li et al., 2018; Panigrahi et al., 2018; Qiao et al., 2020). In BFT system, using different carbon sources, various studies have been conducted, in recent times, which tried to establish a relationship between the bacterial communities of water and gut microbiome of Nile tilapia (Li et al., 2018; Liu et al., 2019; Perez‐Fuentes et al., 2018).…”

Section: Discussionmentioning

confidence: 99%

Study on floc characteristics and bacterial count from biofloc‐based genetically improved farmed tilapia culture system

Yuvarajan

2020

Aquaculture Research

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The present study endeavoured to investigate the efficacy of biofloc technology (BFT) and clear water system (CWS) on nursery rearing of genetically improved farmed tilapia (GIFT). The study used outdoor lined pond (0.01 ha), in duplicate, to explore the bacterial community in culture water and gut flora of GIFT and floc characteristics of both systems. Each pond was stocked with 5,000 numbers (0.2 ± 0.02 g/fish) of GIFT fry and reared for 30 days. Biofloc, with carbon: nitrogen ratio of 10:1, was developed and maintained using distillery spent wash (DSW) as organic carbon source and provided with intensive aeration. Total heterotrophic bacteria (THB), Vibrio and Bacillus count of culture water and gut samples were significantly (p < .05) differed between BFT and CWS. Floc volume, size, settling velocity, porosity and floc volume index were documented from BFT system. Plankton count was significantly differed (p < .05) between the BFT and CWS. BFT showed pleiotropic beneficial effect on rearing medium and species health through colonization of probiotic heterotrophic bacteria and elimination of pathogenic bacteria in the gut. Novelty of floc characteristics measured in the present study would help to form the base clue for the scientific understanding of biofloc system which paves to promote the BFT as sustainable aquaculture practice.

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Poly-β-hydroxybutyrate (PHB) in bioflocs alters intestinal microbial community structure, immune-related gene expression and early Cyprinid herpesvirus 2 replication in gibel carp (Carassius auratus gibelio)

Cited by 39 publications

References 54 publications

Effects of dietary organic acids and nature identical compounds on growth, immune parameters and gut microbiota of European sea bass

Effects of dietary organic acids and nature identical compounds on growth, immune parameters and gut microbiota of European sea bass

Effect of dietary oil fromCamelina sativaon the growth performance, fillet fatty acid profile and gut microbiome of gilthead Sea bream (Sparus aurata)

Study on floc characteristics and bacterial count from biofloc‐based genetically improved farmed tilapia culture system

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