Poly(vinyl alcohol)-hyaluronic Acid Membranes for Wound Dressing Applications: Synthesis andin vitroBio-Evaluations

Abstract

Physically crosslinked poly(vinyl alcohol)-hyaluronic acid (PVA-HA) hydrogel membranes composed of different amounts of HA were prepared by freeze-thawing (F-T) method. F-T cycle was repeated for three consecutive cycles. HA was chosen and routinely utilized in the local treatment of chronic wounds, because of its advantages as, HA is endogenous and biodegradable polymer. Physicochemical properties of PVA-HA membranes such as, gel fraction (GF), swelling, mechanical properties, hydrolytic degradation and in vitro bio-evaluation tests were investigated. Results revealed that introducing HA into PVA structure affected significantly the physicochemical properties of membranes than the pristine PVA, because of its crosslinking interaction with PVA. With the increase of HA content in PVA hydrogel membranes, GF and mechanical stability of PVA-HA membranes decreased. However, the swelling behavior, mechanical flexibility, protein adsorption and hydrolytic degradation of PVA membrane increased. The HA content < 20% in PVA hydrogels showed high cell viability (%) and no toxicity was observed using microculture tetrazolium assay (MTT-assay). However, less cell viability was determined with high HA incorporation. PVA-HA-ampicillin free showed antimicrobial activity against Candida albicans as a result of HA presence. Thus, ampicillin-loaded wound dressing with PVA-HA membranes could be used as promising materials with easy forming and biologically evaluated for wound care. ]]>

Comparative analysis of PVA hydrogels incorporating two natural antimicrobials: Punica granatum and Arnica montana tinctures

“…In samples containing higher amounts of pomegranate, part of the compounds could be attached to the PVA chains anchoring them and it also could diminish the chains entanglement possibility, diminishing the GF, and part of them could be available to be leached out by the saline solution entrance in the swelling test, presenting higher WL . A similar behavior was encountered in PVA‐hyaluronic acid samples, where the increase of hyaluronic acid in the PVA samples increased the hydrolytic degradation . In samples without pomegranate, the PVA chains are not anchored and degradation (breaking of chains entanglement, for example) of the PVA samples could happen, as well as the dissolution of small crystals .…”

Hydrothermal preparation of TiO2-Ag nanoparticles and its antimicrobial performance against human pathogenic microbial cells in water

“…The antimicrobial activity of TiO 2 and TiO 2 -Ag nanoparticles has been tested against Gram positive, Gram negative and a yeast strain, using the disc diffusion method according to Fahmy et al, (2015). At the beginning, both of LB broth and LB agar were prepared and sterilized at 15 psi and 120 o C for 20 minutes.…”

Influence of poloxmer on the dissolution properties of mosapride and its pharmaceutical tablet formulation

“…The doublet band of nearly equal to 1590 and 1620 cm −1 indicated to the ν ring (the inset of Fig .4) [16,25] .…”