Poly(tetrafluoroethylene) separation capillaries for capillary electrophoresisProperties and applications

Macka, M

doi:10.1016/s0021-9673(04)00545-x

Cited by 1 publication

(3 citation statements)

References 31 publications

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“…3). As previously reported in other studies employing fluorinated materials, 16,17 a cathodic EOF is observed highlighting the negative charge of the Dyneon THV surface. At pH 7.4, µ EO of Dyneon THV is 28.3 ± 0.2 × 10 −5 cm 2 V −1 s −1 which is similar to the COC 24 values (26.6 ± 0.1 × 10 −5 cm 2 V −1 s −1 ) at the same pH.…”

Section: Electroosmotic Mobilitysupporting

confidence: 85%

“…Even if from a theoretical point of view, no EOF should be observed on plastic surfaces devoid of ionisable groups, most studies [16][17][18]30 attributed the source of the surface charge and the pH dependence of EOF to impurities resulting from the fabrication process. However, Beattie 31 proposed another explanation for the chemical source of negative charges on the surface of most inert materials.…”

Section: Electroosmotic Mobilitymentioning

confidence: 99%

“…A few studies on capillary electrophoresis (CE) employing fluorinated capillaries have been reported. 15,16 For instance, the simultaneous separation of nine cations and anions on an unmodified fluorinated ethylenepropylene (FEP) capillary has been successfully obtained. 17 Sahlin et al 18 also showed that an in-house made fluorinated capillary allowed a more efficient separation of two cationic aromatic molecules than a fused silica (FS) capillary.…”

Section: Introductionmentioning

confidence: 99%

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Dyneon THV, a fluorinated thermoplastic as a novel material for microchip capillary electrophoresis

Aboud

Ferraro

Taverna

et al. 2016

Analyst

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In this work, we have investigated Dyneon THV, a fluorinated material, as a new material to afford electrokinetic separations in microfluidic devices. To overcome protein adsorption, two poly(ethylene oxide) (PEO)-based coatings have been investigated: Pluronic F127 and PEO stearate 40. The best results were obtained with the PEO stearate 40 coating which allowed decreasing the surface contact angle from 91 ± 3 to 76°± 3. With this surface treatment, a 66% reduction of the electroosmotic mobility at pH 8.0 and a marked suppression of protein adsorption were observed compared to a native Dyneon THV microchip. Finally, a separation of fluorescently labeled proteins (bovine serum albumin and trypsin inhibitor), well-known for their strong tendency to adsorb on hydrophobic surfaces, was successfully achieved in an HEPES buffer with a PEO stearate 40 treated microchip by capillary zone electrophoresis. Furthermore, we demonstrated the possibility to perform non-aqueous capillary electrophoresis analysis of hydrophobic dyes using various solvents in untreated microchips. The overall results demonstrated not only the suitability of the Dyneon THV microchip for electrokinetic separations, but also its versatility allowing different separation modes to be implemented with the same microchip material.

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Section: Electroosmotic Mobilitysupporting

confidence: 85%