Poly(styrene-co-maleic acid)-mediated isolation of supramolecular membrane protein complexes from plant thylakoids

Korotych, Olena; Nguyen, Thao T. T.; Reagan, Brandon C; Burch‐Smith, Tessa M.

doi:10.1016/j.bbabio.2020.148347

Cited by 7 publications

(12 citation statements)

References 81 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…3 ). This contrasts somewhat with previous studies using thylakoid membranes from spinach or from cyanobacteria, where SMA 1440 was considerably more efficient at solubilisation, followed by SMA 30010, SMA 17352, SMA 2625 and then SMA 2000 [42] , [43] , [44] . The reasons for this difference are not clear, but the thylakoid membranes are very densely packed and contain more galactolipid, so differences in polymer-lipid interactions may be responsible.…”

Section: Discussioncontrasting

confidence: 99%

“…A series of partially-esterified variants of SMA has previously been tested for the solubilisation of plant and cyanobacterial thylakoid membranes [33] , [42] , [43] , [44] . These polymers, SMA 2625, SMA 1440 and SMA 17352, have various chemical groups attached to some of the maleic acid groups of SMA ( Fig.…”

Section: Introductionmentioning

confidence: 99%

“…For thylakoid membranes, these partially-esterified polymers, particularly SMA 1440, were more effective than standard SMA. However, thylakoid membranes have quite distinct properties [43] (densely packed and containing high levels of galactolipids), and the performance of these polymers has not yet been investigated for more typical phospholipid membranes from other sources. The esterification within these polymers also means they lack some of the maleic acid groups found in other polymers.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Membrane protein extraction and purification using partially-esterified SMA polymers

Hawkins

Jahromi

Gulamhussein

et al. 2021

Biochimica et Biophysica Acta (BBA) - Biomembranes

View full text Add to dashboard Cite

Styrene maleic acid (SMA) polymers have proven to be very successful for the extraction of membrane proteins, forming SMA lipid particles (SMALPs), which maintain a lipid bilayer around the membrane protein. SMALP-encapsulated membrane proteins can be used for functional and structural studies. The SMALP approach allows retention of important protein-annular lipid interactions, exerts lateral pressure, and offers greater stability than traditional detergent solubilisation. However, SMA polymer does have some limitations, including a sensitivity to divalent cations and low pH, an absorbance spectrum that overlaps with many proteins, and possible restrictions on protein conformational change. Various modified polymers have been developed to try to overcome these challenges, but no clear solution has been found. A series of partially-esterified variants of SMA (SMA 2625, SMA 1440 and SMA 17352) has previously been shown to be highly effective for solubilisation of plant and cyanobacterial thylakoid membranes. It was hypothesised that the partial esterification of maleic acid groups would increase tolerance to divalent cations. Therefore, these partially-esterified polymers were tested for the solubilisation of lipids and membrane proteins, and their tolerance to magnesium ions. It was found that all partially esterified polymers were capable of solubilising and purifying a range of membrane proteins, but the yield of protein was lower with SMA 1440, and the degree of purity was lower for both SMA 1440 and SMA 17352. SMA 2625 performed comparably to SMA 2000. SMA 1440 also showed an increased sensitivity to divalent cations. Thus, it appears the interactions between SMA and divalent cations are more complex than proposed and require further investigation.

show abstract

Section: Discussioncontrasting

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Membrane protein extraction and purification using partially-esterified SMA polymers

Hawkins

Jahromi

Gulamhussein

et al. 2021

Biochimica et Biophysica Acta (BBA) - Biomembranes

View full text Add to dashboard Cite

show abstract

“…Along that theme, the impact of detergent solubilization on our current understanding of PSII function is not well described. A few alternate solubilization techniques that may preserve a more native membrane lipid arrangement, including lipid/protein nanodiscs [ 131 , 132 , 133 ] and SMALPs [ 134 , 135 ] have been explored, but it is an area that warrants more attention.…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Advances in the Understanding of the Lifecycle of Photosystem II

Johnson

Pakrasi

2022

Microorganisms

View full text Add to dashboard Cite

Photosystem II is a light-driven water-plastoquinone oxidoreductase present in cyanobacteria, algae and plants. It produces molecular oxygen and protons to drive ATP synthesis, fueling life on Earth. As a multi-subunit membrane-protein-pigment complex, Photosystem II undergoes a dynamic cycle of synthesis, damage, and repair known as the Photosystem II lifecycle, to maintain a high level of photosynthetic activity at the cellular level. Cyanobacteria, oxygenic photosynthetic bacteria, are frequently used as model organisms to study oxygenic photosynthetic processes due to their ease of growth and genetic manipulation. The cyanobacterial PSII structure and function have been well-characterized, but its lifecycle is under active investigation. In this review, advances in studying the lifecycle of Photosystem II in cyanobacteria will be discussed, with a particular emphasis on new structural findings enabled by cryo-electron microscopy. These structural findings complement a rich and growing body of biochemical and molecular biology research into Photosystem II assembly and repair.

show abstract

“…Bruce and co-workers have recently shown that certain commercially available SMA-based copolymers exhibit the ability to form SMALPs from chloroplast thylakoid membranes, which is of note because of the lack of studies investigating galactolipid-rich membranes. Furthermore, their investigations in cyanobacterial thylakoid membranes showed that commercially available SMA 1440 (Total Cray Valley), a 1.5:1 styrene:maleic acid copolymer that is 72% monoesterified with butoxyethanol, appears to selectively extract photosystem I (PSI) trimer from the thylakoid membranes of Thermosynechococcus elongatus (Te), whereas the most widely used SMA copolymers, those containing a 2:1 ratio of styrene:maleic acid, are ineffective .…”

Section: Introductionmentioning

confidence: 99%

Protein Extraction Efficiency and Selectivity of Esterified Styrene–Maleic Acid Copolymers in Thylakoid Membranes

et al. 2021

Self Cite

View full text Add to dashboard Cite

Amphiphilic styrene−maleic acid copolymers (SMAs) have been shown to effectively extract membrane proteins surrounded by an annulus of native membrane lipids via the formation of nanodiscs. Recent reports have shown that 2butoxyethanol-functionalized SMA derivatives promote the extraction of membrane proteins from thylakoid membranes, whereas unfunctionalized SMA is essentially ineffective. However, it is unknown how the extent of functionalization and identity of sidechains impact protein solubilization and specificity. Herein, we show that the monoesterification of an SMA polymer with hydrophobic alkoxy ethoxylate sidechains leads to an increased solubilization efficiency (SE) of trimeric photosystem I (PSI) from the membranes of cyanobacterium Thermosynechococcus elongatus. The specific SMA polymer used in this study, PRO 10235, cannot encapsulate single PSI trimers from this cyanobacterium; however, as it is functionalized with alkoxy ethoxylates of increasing alkoxy chain length, a clear increase in the trimeric PSI SE is observed. Furthermore, an exponential increase in the SE is observed when >50% of the maleic acid repeat units are monoesterified with long alkoxy ethoxylates, suggesting that the PSI extraction mechanism is highly dependent on both the number and length of the attached side chains.

show abstract

Poly(styrene-co-maleic acid)-mediated isolation of supramolecular membrane protein complexes from plant thylakoids

Cited by 7 publications

References 81 publications

Membrane protein extraction and purification using partially-esterified SMA polymers

Membrane protein extraction and purification using partially-esterified SMA polymers

Advances in the Understanding of the Lifecycle of Photosystem II

Protein Extraction Efficiency and Selectivity of Esterified Styrene–Maleic Acid Copolymers in Thylakoid Membranes

Contact Info

Product

Resources

About