Poly-l-lysine-coated superparamagnetic nanoparticles: a novel method for the transfection of pro-BDNF into neural stem cells

Abstract

Poly-l-lysine-coated superparamagnetic iron oxide nanoparticles (SPIONs-PLL) were prepared and used as a novel-carrier for the transfer of brain-derived neurotrophic factor (BDNF) into neural stem cells (NSCs) under the beneficial influence of an external magnetic field. Pro-BDNF, a gene from human brain cDNA libraries, was obtained by polymerase chain reaction and constructed in a mammalian expression vector (PSecTag2/HygroB). The nanoparticles (NPs) were examined using Fourier transform infrared spectroscopy, zeta potential, and Transmission electron microscopy. From the results, the levels of BDNF among the transfected and untransfected cells were 30.326 ± 5.9 and 5.85 ± 3.11 pg/mL, respectively, as detected by an ELISA method. Moreover, the enhanced green fluorescent protein vector was used to evaluate the gene expression efficiency for SPIONs-PLL as a non-viral carrier in NSCs. This was performed under the influence of a magnetic field and the transfection reagents (such as Lipofectamine 2000), which served as a positive control. The histological analysis revealed that the concentration of intracellular NPs was significantly higher than intercellular NPs. These results suggest that SPIONs-PLL can serve as a novel alternative for the transfection of BDNF-NSCs and could be used in gene therapy.

Green synthesis of silver nanoparticles from Eriobotrya japonica extract: a promising approach against cancer cells proliferation, inflammation, allergic disorders and phagocytosis induction

“…The absorbance for each well was determined at 492 nm on a microplate reader. Regression analysis was used to calculate the concentration of AgNPs required to inhibit 50% of cell growth (IC 50 ) [25].…”

A Novel Microfluidic Device for Blood Plasma Filtration

“…Other microfluidic devices for plasma separation have been demonstrated based on diffusion filter/microfilter [19], bends in micro-channels [20], acoustic waves [21], crossflow filtration [22], and dielectrophoresis [23,24]. Major research projects in recent decades have focused on microfluidics, drug, and gene nano delivery systems, tissue engineering, and biosensors due to cost-effectiveness and high performance [25][26][27][28][29]. Recently, we developed a microfluidic device featuring two nano-junctions with different sizes to form a size and mobility trap (SMT) for on-chip filtrate the whole blood from blood cells and plasma proteins, then on-chip extract, concentrate, and separate small molecules from whole blood [30].…”

Cytotoxic activity of Green Zinc Selenide Nanoparticles Against Hep-G2 Cell Lines

“…It is implemented using a one way (ANOVA) analysis of variance to verify the least significant difference between all treated groups, and (t-test) analysis of variance to verify between contol with each treated groups separately using (Graph Pad Prism 6.00) software. the values were presented as the mean ± SEM oftriplicate measurements 16 .…”