Poly(rC)-binding protein 2 interacts with the oligo(rC) tract of coxsackievirus B3

Zell, Roland; Ihle, Yvonne; Seitz, Simone; Gündel, Ulrike; Wutzler, Peter; Görlach, Matthias

doi:10.1016/j.bbrc.2007.12.038

Cited by 36 publications

(28 citation statements)

References 21 publications

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“…1A). The first domain includes the cloverleaf and the adjacent C-rich spacer, which are required for RNA replication (2)(3)(4). The second domain consists of a large internal ribosomal entry site element that promotes translation of the polyprotein (1, 5-7) (Fig.…”

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confidence: 99%

Identification of two functionally redundant RNA elements in the coding sequence of poliovirus using computer-generated design

Song¹,

Liu²,

Ward³

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

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Genomes of RNA viruses contain multiple functional RNA elements required for translation or RNA replication. We use unique approaches to identify functional RNA elements in the coding sequence of poliovirus (PV), a plus strand RNA virus. The general method is to recode large segments of the genome using synonymous codons, such that protein sequences, codon use, and codon pair bias are conserved but the nucleic acid sequence is changed. Such recoding does not affect the growth of PV unless it destroys the sequence/structure of a functional RNA element. Using genetic analyses and a method called "signal location search," we detected two unique functionally redundant RNA elements (α and β), each about 75 nt long and separated by 150 nt, in the 3′-terminal coding sequence of RNA polymerase, 3Dpol . The presence of wild type (WT) α or β was sufficient for the optimal growth of PV, but the alteration of both segments in the same virus yielded very low titers and tiny plaques. The nucleotide sequences and predicted RNA structures of α and β have no apparent resemblance to each other. In α, we narrowed down the functional domain to a 48-nt-long, highly conserved segment. The primary determinant of function in β is a stable and highly conserved hairpin. Reporter constructs showed that the α-and β-segments are required for RNA replication. Recoding offers a unique and effective method to search for unknown functional RNA elements in coding sequences of RNA viruses, particularly if the signals are redundant in function.computer-designed sequences | chemical DNA synthesis

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confidence: 99%

Identification of two functionally redundant RNA elements in the coding sequence of poliovirus using computer-generated design

Song¹,

Liu²,

Ward³

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

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“…This complex forms with the binding of poly(C) binding protein 1 (PCBP) to stem-loop b and 3CD pr°t o stem-loop d which in turn allows genome circularization (Barton et al, 2001;Gamarnik and Andino, 2000;Herold and Andino, 2001). The ability of TD viruses without stem-loop b to replicate, as exemplified with CVB3-TD50 (which lacks the 5′ nt1-49) (Kim et al, 2005), may be due to the binding of PCBP to the spacer region between domain I and II (Zell et al, 2008), a location that also remains intact in CVB3-TD78. Mutations in the poliovirus domain I (Andino et al, 1990;Trono et al, 1988) also showed loss of CPE (interpreted as lethal) when mutations disrupted stemloop d RNA structure.…”

Section: Resultsmentioning

confidence: 97%

Domain I of the 5′ non-translated genomic region in coxsackievirus B3 RNA is not required for productive replication

et al. 2016

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Domain I is a cloverleaf-like secondary structure at the 5' termini of all enterovirus genomes, comprising part of a cis-acting replication element essential for efficient enteroviral replication. 5' genomic terminal deletions up to as much as 55% of domain I can occur without lethality following coxsackie B virus infections. We report here that the entire CVB structural domain I can be deleted without lethality.

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“…CVB3 is also responsible for numerous other diseases, including aseptic meningitis, pancreatitis, and insulin-dependent diabetes mellitus (4). Despite its well-characterized molecular structure (24), genome functions (38), and certain virus-host interactions (15,19), many aspects of virus-induced pathology remain poorly understood.…”

Section: Introductionmentioning

confidence: 99%

Antibody-Dependent Enhancement of Coxsackievirus B3 Infection of Primary CD19⁺B Lymphocytes

et al. 2010

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Coxsackievirus B3 (CVB3) is associated with several different acute and chronic forms of human disease, including myocarditis, aseptic meningitis, and pancreatitis. Moreover, CVB3 also infects immune cells like CD19+ B lymphocytes, but the viral uptake mechanism into these cells is not well understood. Therefore, primary murine and human CD19+ B cells were isolated by magnetic-activated cell separation technology and analyzed for virus receptor expression, antibody-dependent enhancement of viral infection, and different cellular surface proteins, that might be involved in mechanisms of viral uptake. Western blot analysis of these cells revealed no significant expression of the coxsackievirus-adenovirus receptor CAR. But incubation of CVB3 with serum dilutions, which exhibited binding but not neutralizing characteristics, increased viral uptake and replication significantly in a dose-dependent manner. Viral entry was reduced when Fc portions of immunoglobulins were blocked by protein A treatment. Moreover, the classical complement system rather than Fc-gamma-receptor-mediated mechanisms could be involved in viral uptake. Taken together, these data suggest an antibody-dependent enhancement of CVB3 infection of primary murine and human CD19+ B cells.

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Poly(rC)-binding protein 2 interacts with the oligo(rC) tract of coxsackievirus B3

Cited by 36 publications

References 21 publications

Identification of two functionally redundant RNA elements in the coding sequence of poliovirus using computer-generated design

Identification of two functionally redundant RNA elements in the coding sequence of poliovirus using computer-generated design

Domain I of the 5′ non-translated genomic region in coxsackievirus B3 RNA is not required for productive replication

Antibody-Dependent Enhancement of Coxsackievirus B3 Infection of Primary CD19⁺B Lymphocytes

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Poly(rC)-binding protein 2 interacts with the oligo(rC) tract of coxsackievirus B3

Cited by 36 publications

References 21 publications

Identification of two functionally redundant RNA elements in the coding sequence of poliovirus using computer-generated design

Identification of two functionally redundant RNA elements in the coding sequence of poliovirus using computer-generated design

Domain I of the 5′ non-translated genomic region in coxsackievirus B3 RNA is not required for productive replication

Antibody-Dependent Enhancement of Coxsackievirus B3 Infection of Primary CD19+B Lymphocytes

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Antibody-Dependent Enhancement of Coxsackievirus B3 Infection of Primary CD19⁺B Lymphocytes