Poly (rA) tracts of tumor virus 70S RNA are not transcribed in endogenous or reconstituted reactions of viral reverse transcriptase

Reitz, M. S.; Gillespie, David; Saxinger, W. C.; Robert, Marjorie; Gallo, Robert C.

doi:10.1016/0006-291x(72)90598-0

Cited by 14 publications

(2 citation statements)

References 10 publications

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“…Now that it has been determined that the poly(A) sequences are located at the 3'-OH termini of genomic and subunit RNAs, the possibilty that the poly(A) sequences could be the binding sites for reverse transcriptase increases. However, the poly(A) sequences themselves are apparently not transcribed into tracts of poly(dT) (43). Poly(A) sequences may also be important to the structural integrity of the genomic RNA of RNA-tumor viruses, as has been suggested by others (9)(10)(11), by complementary hydrogen bonding between the poly(A) sequences and possible uridine-rich segments (2,44) in the viral RNA but some investigators have not been able to detect poly(U) tracts in either the genomic or subunit RNA of RNAtumor viruses (45).…”

Section: Discussionmentioning

confidence: 99%

Polyriboadenylate Sequences at the 3′-Termini of Ribonucleic Acid Obtained from Mammalian Leukemia and Sarcoma Viruses

Phillips

Park

Hollis

1974

Proc. Natl. Acad. Sci. U.S.A.

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The location of poly(A) sequences in the RNA of mammalian RNA-tumor viruses was determined by enzymatic analyses. The 56-64S viral genomic RNAs, the 20-40S viral subunit RNAs, and the 4-5S poly(A) sequences excised from these viral RNAs were subjected to either hydrolysis with a 3'-OH specific exoribonuclease from Ehrlich ascites tumor cells or phosphorolysis from the 3'-termini with polynucleotide phosphorylase from Micrococcus luteus. Purified adenosine-labeled poly(A) fragments, excised from genomic viral RNAs by RNase A and T1 digestion, were hydrolyzed with the 3'-OH specific exoribonuclease for various periods of time. Poly(U) filter binding studies of the residual poly(A) indicated that 97% of the poly(A) fragments were hydrolyzed. Adenosinelabeled genomic and subunit viral RNAs and excised poly(A) fragments were phosphorolyzed from their 3'-termini for various periods of time with polynucleotide phosphorylase. The degree of phosphorolysis was monitored by poly(U) filter binding studies, and CC1,COOH insolubility and solubility determinations. There was an initial preferential rate of phosphorolysis of the poly(A) sequences of genomic and subunit viral RNAs as compared to the total adenosine-labeled viral RNAs. The data from these two different enzymatic mechanisms of action indicated conclusively that the poly(A) sequences were located at the 3'-termini of genomic and subunit viral RNAs.

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Section: Discussionmentioning

confidence: 99%

Polyriboadenylate Sequences at the 3′-Termini of Ribonucleic Acid Obtained from Mammalian Leukemia and Sarcoma Viruses

Phillips

Park

Hollis

1974

Proc. Natl. Acad. Sci. U.S.A.

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“…The kinetics of endogenous DNA synthesis by such sucrose density gradient purified virions, in the presence or absence of oligo(dT) and with or without RNase pre-treatment, was then examined (Fig. 5)-DNA synthesis was in all cases approximately linear for at least 2 h and, as in other systems (Duesberg et al I97I;Reitz et al 1972;Sarin & Gallo, I974), was activated by addition of oligo(dT). RNase pre-treatment was again found to enhance the incorporation and this effect was even more profound in the presence ofoligo(dT).…”

Section: The Lcv Endogenous Reactionmentioning

confidence: 93%

Demonstration of an unusual DNA polymerase activity associated with the L cell virion

Hallinan

Lee

Rozee

1979

Journal of General Virology

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SUMMARYPurified preparations of L cell virions (LCV) were found to: possess an associated DNA polymerase activity. This enzyme was active with poly(C), oligo(dG) and poly(Cm), oligo(dG) and was able to transcribe poly(A), oligo(dT). Endogenous DNA synthesis was also demonstrable in disrupted virion preparations but this reaction was enhanced, rather than inhibited, by RNase pre-treatment. The effects of variations in a number of the assay parameters on these activities were examined in an attempt to determine the class of DNA polymerase involved.

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Inhibitors of DNA Synthesis in RNA Tumor Viruses: Biological Implications and Their Mode of Action

Prakash¹,

Steel²,

Ebener³

et al. 1976

Progress in Molecular and Subcellular Biology

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Poly (rA) tracts of tumor virus 70S RNA are not transcribed in endogenous or reconstituted reactions of viral reverse transcriptase

Cited by 14 publications

References 10 publications

Polyriboadenylate Sequences at the 3′-Termini of Ribonucleic Acid Obtained from Mammalian Leukemia and Sarcoma Viruses

Polyriboadenylate Sequences at the 3′-Termini of Ribonucleic Acid Obtained from Mammalian Leukemia and Sarcoma Viruses

Demonstration of an unusual DNA polymerase activity associated with the L cell virion

Inhibitors of DNA Synthesis in RNA Tumor Viruses: Biological Implications and Their Mode of Action

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