Poly[oligo(ethylene glycol) methacrylate‐<i>co</i>‐glycidyl methacrylate] Brush Substrate for Sensitive Surface Plasmon Resonance Imaging Protein Arrays

Hu, Weihua; Liu, Yingshuai; Lu, Zhisong; Li, Chang Ming

doi:10.1002/adfm.201001159

Cited by 94 publications

(89 citation statements)

References 47 publications

(60 reference statements)

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“…All recombinant proteins were purified from Escherichia coli BL21. The oblique-incidence reflectivity difference (OIRD) method was performed as described previously (26,27). BSA and recombinant Hsp90 proteins were placed on processed glass chips by use of Microarray Spotter and then immobilized for 12 h at 25°C.…”

Section: Methodsmentioning

confidence: 99%

Tom70 Mediates Sendai Virus-Induced Apoptosis on Mitochondria

Wei

Cui

Huang

et al. 2015

J Virol

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Virus infection triggers immediate innate immune responses. Apoptosis represents another effective means to restrict virus invasion, besides robust expression of host cytokines and chemokines. IRF3 was recently demonstrated to be indispensable for Sendai virus (SeV)-induced apoptosis, but the underlying mechanism is not fully understood. Here we report that a dynamic protein complex, Tom70/Hsp90/IRF3/Bax, mediates SeV-induced apoptosis. The cytosolic proapoptotic protein Bax interacts specifically with IRF3 upon virus infection. The mitochondrial outer membrane protein Tom70 recruits IRF3 to mitochondria via Hsp90. Consequently, the relocation of Bax onto mitochondria induces the leakage of cytochrome c into the cytosol and initiates the corresponding apoptosis. Interestingly, IKK-i is essential for this apoptosis, whereas TBK1 is dispensable. Collectively, our study characterizes a novel protein complex that is important for SeV-induced apoptosis. IMPORTANCEApoptosis is an effective means of sacrificing virus-infected cells and restraining the spread of virus. In this study, we demonstrate that IRF3 associates with Bax upon virus infection. Tom70 recruits this protein complex to the mitochondrial outer membrane through Hsp90, which thus induces the release of cytochrome c into the cytosol, initiating virus-induced apoptosis. Interestingly, IKK-i plays an essential role in this activation. This study uncovers a novel mechanism of SeV-induced apoptosis.

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Section: Methodsmentioning

confidence: 99%

Tom70 Mediates Sendai Virus-Induced Apoptosis on Mitochondria

Wei

Cui

Huang

et al. 2015

J Virol

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“…However, the low concentration of biomarkers in urine begs the need for highly sensitive devices for accurate detection. To detect low concentrations, biosensors often employ complex features such as nanoparticles [1][2][3] and structured surfaces [4][5][6] with equipment intensive amplifi cation methods such as surface plasmon resonance [7][8][9] to enhance the detection signal. Another approach for enhanced detection is increasing the concentration of biomolecules to a range detectable using standard techniques (such as a colorimetric output).…”

Section: Introductionmentioning

confidence: 99%

Enhanced Detection of Protein in Urine by Droplet Evaporation on a Superhydrophobic Plastic

McLane

Khine

2014

Adv Materials Inter

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than wet the surface [10][11][12][13][14][15][16][17][18][19][20][21][22][23] due to the high surface tension of water, the low surface energy of the substrate, and the minimal adhesion between water and the surface. The low surface energy and minimal adhesion can be attributed to multiscale features, ranging from micro to nano, [ 11 ] that trap air pockets between the surface and water, allowing the water droplet to only contact the peaks of the multiscale structures. The multiscale features can be highly patterned (homogeneous) or random (heterogeneous) but need to be multi-to nanoscale to trap air between the surface and fl uid. Such SH surfaces have been used for anti-fouling applications and drug delivery. [ 20,[24][25][26][27] When air is trapped between water and the surface, the surface is in the Cassie-Baxter regime, [ 10 ] and the water droplet's adhesion to the surface is poor. A droplet in the Cassie-Baxter regime can transition to the Wenzel regime (no air pockets and strong adhesion to the surface) [ 28 ] when the balance of forces is disrupted. [ 29 ] Pressure can disrupt this balance and change a water droplet from balancing on the peaks (Cassie) to sinking into the multiscale structures (Wenzel). [30][31][32][33] A water droplet can naturally transition from Cassie to Wenzel due to a change in internal droplet pressure, which can be quantifi ed bywhere γ is the surface tension of the fl uid, and R is the radius of the droplet. [ 30,34 ] Evaporation of a droplet can cause the Cassie to Wenzel transition due to the decrease in volume and increase in internal pressure. When a droplet of fl uid evaporates into the atmosphere, the balance of forces at the air-liquid interface constantly increases the droplet's surface tension and applies an inward force at the droplet's contact line (air-liquid-solid interface). [ 35 ] On a SH surface, the droplet's inward pulling force is greater than the droplet's adhesion to the surface, and the droplet's contact line continually moves inward, creating a smaller footprint and contact diameter (CD) for the droplet, [36][37][38][39][40][41] as shown in Figure 1 . Eventually, the droplet collapses to its fi nal footprint when the internal Laplace pressure overcomes the upward force from the air pockets, [ 30,31 ] and fl uid will stay pinned at this reduced contact area until evaporation is complete. This reduced contact area contains the content of the droplet with the molecules concentrated only on a small footprint.Protein in urine can be detected using a simple colorimetric output by evaporating droplets on a superhydrophobic (SH) surface. Evaporation on a SH surface allows fl uid to dramatically concentrate; the weak surface adhesion allows the droplet of fl uid to constantly decrease its footprint area and contact diameter. On a SH surface, pure water completely evaporates. Molecules in solution, however, are confi ned to a footprint that is 8.5 times smaller than the original and are greatly concentrated. By concentrating molecules, a 160 times improved detectio...

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“…The nonspecific protein adsorption could significantly affect the detection limit of an antibody microarray in biological fluids such as blood and serum due to various interferences (Hu et al, 2007(Hu et al, , 2008(Hu et al, , 2010b. In this work concentrated BSA solution was employed to block the GPTS-modified ZnO surface for reducing the interferences.…”

Section: Resultsmentioning

confidence: 99%