Poly(N-isopropylacrylamide) hydrogels with interpenetrating multiwalled carbon nanotubes for cell sheet engineering

Chen, Yu-Shuan; Tsou, Pei-Chun; Lo, Jason; Tsai, Hsieh‐Chih; Wang, Yanzhen; Hsiue, Ging‐Ho

doi:10.1016/j.biomaterials.2013.06.017

Cited by 90 publications

(63 citation statements)

References 31 publications

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“…A freeze drying method was used to generate a honeycomb structure and observe changes in gel structure with CNT addition. 66 The larger pore size on the other hand could be related to the overall increase in swelling. According to the mesh size calculated earlier, we expected that the pore size of the hydrogel samples would be in the nanomolar range.…”

Section: Characterization Of Peg-cnt Composite Hydrogels Swelling Andmentioning

confidence: 99%

Development and characterization of polyethylene glycol–carbon nanotube hydrogel composite

et al. 2015

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Carbon nanotube (CNT)-hydrogel composites are attractive for a variety of neural tissue engineering and drug delivery applications as well as biosensor coatings, transducers and leads.Both materials contribute unique and beneficial properties to the composites. Hydrogels are an excellent mimic of the extracellular matrix due to their hydrophilicity, viscoelasticity and biocompatibility. CNTs, on the other hand, can impart electroconductivity to otherwise insulating materials, improve mechanical stability and guide neuronal cell behavior as well as elicit axon regeneration. Not surprisingly, there has been a surge in the development of various CNT-hydrogel composites including both natural and synthetic polymers. Here, we describe a CNT-polyethylene glycol (PEG) hydrogel composite where the CNTs are entrapped in the hydrogel phase during gelation. The hydrogel crosslinking reaction is based on Michael-type addition which is ideal for in situ cell and protein encapsulation. To adequately disperse the highly hydrophobic CNTs in the aqueous polymer solution, we used sonication and surfactants, where bovine serum albumin was found to be an effective and non-cytotoxic dispersant. We demonstrate that the inclusion of the CNTs impeded the hydrogel crosslinking leading to longer gelation times, higher swelling and porosity, and lower storage modulus above a threshold CNT concentration. As anticipated, composite hydrogel resistivity decreased with the incorporation of CNTs and was dependent on both CNT loading and dispersion. Importantly, unlike the PEG hydrogel alone, the PEG-CNT hydrogel composite was capable of supporting high neural cell viability where the CNTs provided sites for cell attachment.

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Section: Characterization Of Peg-cnt Composite Hydrogels Swelling Andmentioning

confidence: 99%

Development and characterization of polyethylene glycol–carbon nanotube hydrogel composite

et al. 2015

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“…[35][36][37][38] However, very limited work has been conducted on the use of CNTs as scaffold components in canine models, such as the Madin-Darby Canine Kidney epithelial cell (MDCK) attachment study on multiwalled CNT (MWCNT)-incorporated poly(N-isopropylacrylamide) (PNIPAAM) hydrogel, primary canine chondrocyte proliferation on aerogel network of CNTs, and use of CNT-coated stent as delivery vehicle, to describe a few. [39][40][41] The recent elucidation of accelerated in vitro osteogenesis of human preosteoblasts and murine ESCs on carboxylated CNT-coated coverslips followed by ectopic bone formation in a rat model has opened up the window of continuing further investigations on CNTs in other models. 42 Considering the potential importance of CNTs as a scaffold material in human and veterinary medicine, it is necessary to achieve further development in the field and attain new knowledge.…”

Section: Introductionmentioning

confidence: 99%

Functionalized carbon nanotubes as suitable scaffold materials for proliferation and differentiation of canine mesenchymal stem cells

Das¹,

Madhusoodan²,

Mili³

et al. 2017

IJN

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In the field of regenerative medicine, numerous potential applications of mesenchymal stem cells (MSCs) can be envisaged, due to their ability to differentiate into a range of tissues on the basis of the substrate on which they grow. With the advances in nanotechnology, carbon nanotubes (CNTs) have been widely explored for use as cell culture substrate in tissue engineering applications. In this study, canine bone marrow-derived MSCs were considered as the cellular model for an in vitro study to elucidate the collective cellular processes, using three different varieties of thin films of functionalized carbon nanotubes (COOH-single-walled CNTs [SWCNTs], COOH-multiwalled CNTs [MWCNTs] and polyethylene glycol [PEG]-SWCNTs), which were spray dried onto preheated cover slips. Cells spread out better on the CNT films, resulting in higher cell surface area and occurrence of filopodia, with parallel orientation of stress fiber bundles. Canine MSCs proliferated at a slower rate on all types of CNT substrates compared to the control, but no decline in cell number was noticed during the study period. Expression of apoptosis-associated genes decreased on the CNT substrates as time progressed. On flow cytometry after AnnexinV-fluorescein isothiocyanate/propidium iodide (PI) staining, total number of apoptotic and necrotic cells remained lower in COOH-functionalized films compared to PEG-functionalized ones. Collectively, these results indicate that COOH-MWCNT substrate provided an environment of low cytotoxicity. Canine MSCs were further induced to differentiate along osteogenic, chondrogenic, and neuronal lineages by culturing under specific differentiation conditions. The cytochemical and immunocytochemical staining results, as well as the expression of the bone marker genes, led us to hypothesize that the COOH-MWCNT substrate acted as a better cue, accelerating the osteogenic differentiation process. However, while chondrogenesis was promoted by COOH-SWCNT, neuronal differentiation was promoted by both COOH-SWNCT and COOH-MWCNT. Taken together, these findings suggest that COOH-functionalized CNTs represent a promising scaffold component for future utilization in the selective differentiation of canine MSCs in regenerative medicine.

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“…The preparation process and the type of carbon filler used determine the interactions between the filler particles as well as between the fillers and the polymer matrix [28]. Investigations regarding CNT [29][30][31] and GO [32][33][34] containing PNIPA based composite systems show improved mechanical properties (elastic modulus and compressive strength). As these gels were investigated with a variety of cross-link densities and/or with different co-monomers, as well as diverse conditions of synthesis, it is not possible to evaluate the effects of the different nanoparticles.…”

Section: Introductionmentioning

confidence: 99%

Thermal sensitivity of carbon nanotube and graphene oxide containing responsive hydrogels

Manek

Berke

Miklósi

et al. 2016

Express Polym. Lett.

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Abstract.Comparative investigations are reported on poly(N-isopropylacrylamide) (PNIPA) gels of various carbon nanotube (CNT) and graphene oxide (GO) contents synthesized under identical conditions. The kind and concentration of the incorporated carbon nanoparticles (CNPs) influence the swelling and stress-strain behaviour of the composites. Practically independently of the filler content, incorporation of CNPs appreciably improves the fracture stress properties of the gels. The time constant and the swelling ratio of the shrinkage following an abrupt increase in temperature of the swelling medium from 20 to 50°C can be adjusted by selecting both the type and the amount of nanoparticle loading. This offers a means of accurately controlling the deswelling kinetics of drug release with PNIPA systems, and could be employed in sensor applications where fast and excessive shrinkage are a significant drawback. Both CNTs and GO enhance the infrared sensitivity of the PNIPA gel, thus opening a route for the design of novel drug transport and actuator systems. It is proposed that the influence of the CNPs depends more on their surface reactivity during the gel synthesis rather than on their morphology. One of the important findings of this study is the existence of a thermally conducting network in the GO filled gels.

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Poly(N-isopropylacrylamide) hydrogels with interpenetrating multiwalled carbon nanotubes for cell sheet engineering

Cited by 90 publications

References 31 publications

Development and characterization of polyethylene glycol–carbon nanotube hydrogel composite

Development and characterization of polyethylene glycol–carbon nanotube hydrogel composite

Functionalized carbon nanotubes as suitable scaffold materials for proliferation and differentiation of canine mesenchymal stem cells

Thermal sensitivity of carbon nanotube and graphene oxide containing responsive hydrogels

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