Poly (N-isopropylacrylamide)-functionalized dendrimer as a thermosensitive nanoplatform for delivering malloapelta B against HepG2 cancer cell proliferation

Abstract

In recent years, nanocarriers have emerged as effective platforms for delivering several kinds of herbal medicine and naturally bioactive compounds. In this study we developed an outstanding thermosensitive dendritic nanocarrier to efficiently deliver malloapelta B (Mall B), which is a water insoluble bioactive compound isolated from leaves of Mallotus apelta-Vietnamese medicinal plant. The thermosensitive poly(N-isopropylacrylamide) (PNIPAM) polymer-conjugated polyamidoamine (PAMAM) dendrimer copolymer was prepared via Michael reaction. The copolymer structures were confirmed by proton nuclear magnectic resonance ( 1 H NMR). Morphology of the nanocarrier was observered around 70-120 nm by transmission electron microscopy (TEM). Size distributions were measured by dynamic light scattering (DLS) of the nanocarrier and its Mall B-loaded performed at 146.8 nm and 194.5 nm, respectively. The PNIPAM-g-PAMAM-based nanocarrier exhibited higher Mall B loading efficiency (DL = 59.93 ± 0.19%) and entrapment efficiency (EE = 89.98 ± 2.06%) as compared to PNIPAM (DL = 52.54 ± 0.45% and EE = 66.45 ± 2.78%). In vitro release indicated that approximately 30% amount of the loaded Mall B released at pH 5.5 after 54 h tracking. At the same time, 12.5% amount of the molecules released at pH 7.4.Cytotoxicity assay results showed that the Mall B-loaded nanocarrier significantly inhibited HepG2 cancer cell proliferation. These obtained results indicated that the nanocarrier could solve hydrophobic property of Mall B for further medicine applications.

Injectable Hydrogels for Cardiac Tissue Engineering

“…Poly( N ‐isopropylacrylamide) (PNIPAAm) is a thermosensitive water‐soluble homopolymer that has garnered a lot of attention in the biomedical field due to its sharp, reversible solution‐to‐gelation (sol‐to‐gel) transition point of 32 °C being sufficiently high to enable it to be a solution at room temperature, yet still low enough so that it becomes a gel at body temperature, making it extremely useful for many biomedical applications . When conjugated with other polymers, PNIPAAm may impart its thermosensitive behavior on otherwise non‐thermosensitive polymers . Thus, this polymer system may be injected into the treatment site and will conform to the irregularities of the injury site via in situ gelation.…”

“…[147,148] When conjugated with other polymers, PNIPAAm may impart its thermosensitive behavior on otherwise non thermosensitive polymers. [149][150][151] Thus, this polymer system may be injected into the treatment site and will conform to the irregularities of the injury site via in situ gelation. PNIPAAm based polymer systems have been used for cardiac tissue engi neering approaches as drug delivery systems, cell scaffolding, and transplantation, and in vitro cell culture applications.…”

Synthesis of a Series of Folate-Terminated Dendrimer-b-PNIPAM Diblock Copolymers: Soft Nanoelements That Self-Assemble into Thermo- and pH-Responsive Spherical Nanocompounds

“…There are several reports on different types of dendritic conjugates with PNIPAM and a few examples about dendrimer-PNIPAM conjugates. − However, to date, there is no systematic study on the structure–activity relationship on a series of dendrimer- b -PNIPAM copolymers comparing several generations of dendrimers or the use of dendrimers with the 1 → 3 connectivity that are more dense than 1 → 2 dendrimers. Herein, we report the synthesis of a series of dendrimer- b -PNIPAM diblock copolymers bearing folate termini and the study of their self-assembly process.…”