Poly-(Lactic-co-Glycolic) Acid Nanoparticles for Synergistic Delivery of Epirubicin and Paclitaxel to Human Lung Cancer Cells

Sharma, Nikita; Kumari, Rajendra; Gupta, Nidhi; Syed, Asad; Bahkali, Ali H.; Nimesh, Surendra

doi:10.3390/molecules25184243

Cited by 24 publications

(21 citation statements)

References 48 publications

(62 reference statements)

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“…This resists the fast diffusion of the drug from the particle and maintained its sustained release for long hours. In one of our earlier publications, polyethylenimine-modified PLGA nanoparticles also showed maximum release of epirubicin and paclitaxel at pH 5.4 as compared to pH 7.6 [ 36 ].…”

Section: Resultsmentioning

confidence: 99%

“…The results further corroborated the nuclear changes and apoptosis, which were visualized microscopically. Our previous study also showed induction of membrane blebbing and chromatin condensation, as signs of early apoptosis when A549 cells were treated with EPI-PTX combination drug and modified PLGA nanoparticles entrapping these drugs [ 36 ].…”

Section: Resultsmentioning

confidence: 99%

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Diosgenin Loaded Polymeric Nanoparticles with Potential Anticancer Efficacy

Sharma

Singhal²,

Kumari

et al. 2020

Biomolecules

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This study aims to determine the anticancer efficacy of diosgenin encapsulated poly-glycerol malate co-dodecanedioate (PGMD) nanoparticles. Diosgenin loaded PGMD nanoparticles (variants 7:3 and 6:4) were synthesized by the nanoprecipitation method. The synthesis of PGMD nanoparticles was systematically optimized employing the Box-Behnken design and taking into account the influence of various independent variables such as concentrations of each PGMD, diosgenin and PF-68 on the responses such as size and PDI of the particles. Mathematical modeling was done using the Quadratic second order modeling method and response surface analysis was undertaken to elucidate the factor-response relationship. The obtained size of PGMD 7:3 and PGMD 6:4 nanoparticles were 133.6 nm and 121.4 nm, respectively, as measured through dynamic light scattering (DLS). The entrapment efficiency was in the range of 77–83%. The in vitro drug release studies showed diffusion and dissolution controlled drug release pattern following Korsmeyer–Peppas kinetic model. Furthermore, in vitro morphological and cytotoxic studies were performed to evaluate the toxicity of synthesized drug loaded nanoparticles in model cell lines. The IC50 after 48 h was observed to be 27.14 µM, 15.15 µM and 13.91 µM for free diosgenin, PGMD 7:3 and PGMD 6:4 nanoparticles, respectively, when administered in A549 lung carcinoma cell lines.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Diosgenin Loaded Polymeric Nanoparticles with Potential Anticancer Efficacy

Sharma

Singhal²,

Kumari

et al. 2020

Biomolecules

Self Cite

View full text Add to dashboard Cite

show abstract

“…Avoiding the post-printing crosslinking step would make this method more suitable for the preparation of drug-delivery platforms for small hydrophilic molecules. To present a proof of principle, we decided to load our 3D-printed platforms with epirubicin-HCl (EPI), a water-soluble 4′epi-isomer of doxorubicin with a well-established application in breast-cancer treatment, one of the most prevalent diseases among women worldwide [ 20 , 21 ]. EPI induces DNA damage by inhibiting the topoisomerase II activity, inducing apoptosis in cancer cells [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

Alginate Self-Crosslinking Ink for 3D Extrusion-Based Cryoprinting and Application for Epirubicin-HCl Delivery on MCF-7 Cells

et al. 2022

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3D-printed hydrogels are particularly advantageous as drug-delivery platforms but their loading with water-soluble active compounds remains a challenge requiring the development of innovative inks. Here, we propose a new 3D extrusion-based approach that, by exploiting the internal gelation of the alginate, avoids the post-printing crosslinking process and allows the loading of epirubicin-HCl (EPI). The critical combinations of alginate, calcium carbonate and d-glucono-δ-lactone (GDL) combined with the scaffold production parameters (extrusion time, temperature, and curing time) were evaluated and discussed. The internal gelation in tandem with 3D extrusion allowed the preparation of alginate hydrogels with a complex shape and good handling properties. The dispersion of epirubicin-HCl in the hydrogel matrix confirmed the potential of this self-crosslinking alginate-based ink for the preparation of 3D-printed drug-delivery platforms. Drug release from 3D-printed hydrogels was monitored, and the cytotoxic activity was tested against MCF-7 cells. Finally, the change in the expression pattern of anti-apoptotic, pro-apoptotic, and autophagy protein markers was monitored by liquid-chromatography tandem-mass-spectrometry after exposure of MCF-7 to the EPI-loaded hydrogels.

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“…When the temperature decreases from 40 to 20 • C, the time to reach adsorption equilibrium is prolonged from 90 to 400 min, while the loading rate at adsorption equilibrium increases from 24.83 to 36.14 mg•g −1 . It should be noted that the loading rate of PTX on CA-CSNPs was higher than that on polylactide-hydroxyacetic acid nanoparticles (21.16 mg•g −1 ) [44]. As shown in Table 3, the adsorption equilibrium constant (K L or K C ) also decreases with the increasing of temperature.…”

Section: Drug Loading Analysismentioning

confidence: 87%

Preparation and Drug-Loading Properties of Amphoteric Cassava Starch Nanoparticles

et al. 2022

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Based on the characteristics of charge reversal around the isoelectric point (pI) of amphoteric starch-containing anionic and cationic groups, amphoteric cassava starch nanoparticles (CA-CANPs) are prepared by a W/O microemulsion crosslinking method using (3-chloro-2-hydroxypropyl) trimethyl ammonium chloride as a cationic reagent and POCl3 as an anionic reagent, and the effects of preparation conditions on the particle size of the CA-CANPs are studied in detail in the present study. CA-CANPs with a smooth surface and an average diameter of 252 nm are successfully prepared at the following optimised conditions: a crosslinking agent amount of 15 wt%, an aqueous starch concentration of 6.0 wt%, an oil–water ratio of 10:1, a total surfactant amount of 0.20 g·mL−1, and a CHPTAC amount of 4.05 wt%. The pH-responsive value of the CA-CANPs can be regulated by adjusting the nitrogen–phosphorus molar ratio in the CA-CANPs. By using CA-CANPs with a pI of 6.89 as drug carriers and the paclitaxel (PTX) as a model drug, the maximum loading rate of 36.14 mg·g−1 is achieved, and the loading process is consistent with the Langmuir isotherm adsorption, with the calculated thermodynamic parameters of ΔH° = −37.91 kJ·mol−1, ΔS° = −10.96 J·mol−1·K−1 and ΔG° < 0. By testing the release rate in vitro, it is noted that the release rates of PTX in a neutral environment (37.6% after 96 h) and a slightly acidic environment (58.65% after 96 h) are quite different, suggesting that the CA-CANPs have the possibility of being a targeted controlled-release carrier with pH responsiveness for antitumor drugs.

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Poly-(Lactic-co-Glycolic) Acid Nanoparticles for Synergistic Delivery of Epirubicin and Paclitaxel to Human Lung Cancer Cells

Cited by 24 publications

References 48 publications

Diosgenin Loaded Polymeric Nanoparticles with Potential Anticancer Efficacy

Diosgenin Loaded Polymeric Nanoparticles with Potential Anticancer Efficacy

Alginate Self-Crosslinking Ink for 3D Extrusion-Based Cryoprinting and Application for Epirubicin-HCl Delivery on MCF-7 Cells

Preparation and Drug-Loading Properties of Amphoteric Cassava Starch Nanoparticles

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