Poly(lactic acid) nanoparticles loaded with ursolic acid: Characterization and in vitro evaluation of radical scavenging activity and cytotoxicity

Antônio, Emilli; Antunes, Osmar dos Reis; Araújo, Isis Souza de; Khalil, Najeh Maissar; Mainardes, Rubiana Mara

doi:10.1016/j.msec.2016.09.080

Cited by 25 publications

(12 citation statements)

References 69 publications

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“…The DSC signature for GLP had a first peak at 101.2°C and a second peak at 281.67°C. It is of note that the endothermic peak at 307.1°C for genistein was not found in GLP (Figure 9(c)), indicating the amorphous state of genistein in GLP [11].…”

Section: Journal Of Nanomaterialsmentioning

confidence: 97%

“…Nanotechnology has allowed incorporating new pharmaceuticals into different types of nanoparticles (liposomes and emulsions) and improving drug properties, particularly solubility and bioavailability [11][12][13][14][15]. Nanoparticles are prepared using a variety of polymers, including hydrophilic polyethylene glycol (PEG), hydrophobic polylactic acid (PLA), and amphiphilic PEG-PLA block copolymers [16].…”

Section: Introductionmentioning

confidence: 99%

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Preparation and Characterization of PEG-PLA Genistein Micelles Using a Modified Emulsion-Evaporation Method

Cheng

Qin

et al. 2020

Journal of Nanomaterials

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The objective of this study is to improve the bioavailability of genistein by encapsulation with polyethylene glycol-polylactic acid (PEG-PLA) copolymers. Genistein micelles (GMs) prepared using a modified emulsion-evaporation method were more stable than those made with the original method. The effect of polyvinyl alcohol, Tween 80, sonication time, PEG-PLA/genistein ratio, and organic phase (acetone)/H2O ratio on the size, polydispersity index, encapsulation efficiency, and drug loading efficiency of GMs was investigated. GMs were obtained and characterized under optimal experimental conditions. For long-term storage, GMs were lyophilized by freeze drying with trehalose to produce genistein lyophilized powder (GLP). The analysis of GLP by Fourier-transform infrared spectroscopy and differential scanning calorimetry showed that genistein was successfully incorporated into the micellar structure. In vitro release experiments revealed that the incorporation of genistein into PEG-PLA copolymers significantly improved its solubility and bioavailability. GLP was more potent in inhibiting the proliferation of HSC-T6 cells than genistein. Treatment with GLP at 10–20 μg/mL for 48 h significantly inhibited the protein expression of α-smooth muscle actin and collagen I in HSC-T6 cells compared with the control. These data demonstrated that the improved solubility and bioavailability of genistein in the form of GLP enhanced its antifibrotic effect in vitro.

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Section: Journal Of Nanomaterialsmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Preparation and Characterization of PEG-PLA Genistein Micelles Using a Modified Emulsion-Evaporation Method

Cheng

Qin

et al. 2020

Journal of Nanomaterials

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“…The encapsulation efficiency was in all cases over 68%, reaching even 98%, for drug loading equal to 60%. An emulsification-solvent evaporation technique was also used [81] to develop PLA NPs containing ursolic acid and evaluate the radical scavenging activity over hypochlorous acid (HOCl) and cytotoxicity on erythrocytes and tumor cells. Ursolic acid is a natural antioxidant with anti-inflammatory, antihyperlipidemic, antidiabetic, hepatoprotective, neuroprotective, antiobesity, and anticancer properties.…”

Section: Poly-dl-lactide-based Nanoparticlesmentioning

confidence: 99%

Nanoantioxidants: Recent Trends in Antioxidant Delivery Applications

et al. 2019

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Antioxidants interact with free radicals, terminating the adverse chain reactions and converting them to harmless products. Antioxidants thus minimize the oxidative stress and play a crucial role in the treatment of free radicals-induced diseases. However, the effectiveness of natural and/or synthetic antioxidants is limited due to their poor absorption, difficulties to cross the cell membranes, and degradation during delivery, hence contributing to their limited bioavailability. To address these issues, antioxidants covalently linked with nanoparticles, entrapped in nanogel, hollow particles, or encapsulated into nanoparticles of diverse origin have been used to provide better stability, gradual and sustained release, biocompatibility, and targeted delivery of the antioxidants with superior antioxidant profiles. This review aims to critically evaluate the recent scientific evaluations of nanoparticles as the antioxidant delivery vehicles, as well as their contribution in efficient and enhanced antioxidant activities.

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“…In addition, analyses were performed on chitosan films without MHF (BF) for comparison purposes. The infrared spectrum of MHF ( Figure 2 and Figure S1 ) shows bands characteristic ( Table 3 ) of triterpenoids [ 35 , 36 , 37 , 38 ]. For the BF, the spectrum showed the characteristics absorption bands of chitosan as previously reported [ 39 , 40 , 41 ].…”

Section: Resultsmentioning

confidence: 99%

“…The amorphous nature of CMHF is an indication of good miscibility between the components [43]. Angular deformation vibration of CH alkenes [38] 1372 cm −1 Acetamide groups [39] 1150 cm −1 Anti-symmetric stretching of the C-O-C bridge [41] 1075 cm −1 Skeletal vibrations involving the C-O stretching; [41] 1029 and 997 cm −1 Symmetric C-O stretches and olefinic groups [36] 890 cm −1 Vibration of C-C skeleton [41] 3.2.…”

Section: Xrdmentioning

confidence: 99%

Chitosan Film Containing Mansoa hirsuta Fraction for Wound Healing

et al. 2020

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Chitosan films entrapped with the Mansoa hirsuta fraction (CMHF) was developed as a new dressing for wound care. The chromatographic profile of the M. hirsuta fraction (MHF) was evaluated by ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry, and the results showed that MHF is rich in acid triterpenes. Physicochemical characterization of the films prepared using the solvent casting method was performed by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermogravimetry (TGA), differential scanning calorimetry (DCS), scanning electron microscopy (SEM), atomic force microscopy (AFM), and mechanical properties. CMHF exhibited characteristic bands of both chitosan and MHF, revealing a physical mixture of both. CMHF presented an amorphous nature, thermostability, and dispersion of MHF in the chitosan matrix, resulting in a rough structure. Incorporation of M. hirsuta fraction into chitosan matrix favorably enhanced the mechanical performance and films thickness. The in vivo wound treatment with CMHF for seven days showed a characteristic area of advanced healing, re-epithelization, cell proliferation, and collagen formation. Furthermore, wound closure reached 100% contraction after 10 days of treatment with modulation of interleukins. The incorporation of M. hirsuta fraction into chitosan films was advantageous and showed great potential for stimulating wound repair and regeneration.

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Poly(lactic acid) nanoparticles loaded with ursolic acid: Characterization and in vitro evaluation of radical scavenging activity and cytotoxicity

Cited by 25 publications

References 69 publications

Preparation and Characterization of PEG-PLA Genistein Micelles Using a Modified Emulsion-Evaporation Method

Preparation and Characterization of PEG-PLA Genistein Micelles Using a Modified Emulsion-Evaporation Method

Nanoantioxidants: Recent Trends in Antioxidant Delivery Applications

Chitosan Film Containing Mansoa hirsuta Fraction for Wound Healing

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