Poly (lactic acid)–chitosan–collagen composite nanofibers as substrates for blood outgrowth endothelial cells

Swarnalatha, B.; Nair, Sethu L; Shalumon, K.T.; Milbauer, Liming; Jayakumar, R.; Paul‐Prasanth, Bindhu; Menon, Krishnakumar N.; Hebbel, Robert P.; Somani, Arif; Nair, Shantikumar V.

doi:10.1016/j.ijbiomac.2013.03.060

Cited by 26 publications

(17 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A low toxicity among cells cultured on chitosan/collagen nanoscaffolds has been noted in other studies on culture of bone (Levengood & Zhang, 2014), cartilage (Gong et al, 2010), skin (Sarkar et al, 2013), intestinal smooth muscle (Zakhem et al, 2012) and endothelial cells (Swarnalatha et al, 2013).…”

Section: Discussionmentioning

confidence: 56%

Effects of 3-dimensional culture conditions (collagen-chitosan nano-scaffolds) on maturation of dendritic cells and their capacity to interact with T-lymphocytes

Daneshmandi

Dibazar

Fateh

2015

Journal of Immunotoxicology

View full text Add to dashboard Cite

In the body, there is a natural three-dimensional (3D) microenvironment in which immune cells, including dendritic cells (DC), play their functions. This study evaluated the impact of using collagen-chitosan 3D nano-scaffolds in comparisons to routine 2D culture plates on DC phenotype and functions. Bone marrow-derived DC were cultured on scaffolds and plates and then stimulated with lipopolysaccharide (LPS) or chitosan-based nanoparticles (NP) for 24 h. Thereafter, DC viability, expression of maturation markers and levels of cytokines secretion were evaluated. In another set of studies, the DC were co-cultured with allogenic T-lymphocytes in both the 2D and 3D systems and effects on DC-induction of T-lymphocyte proliferation and cytokine release were analyzed. The results indicated that CD40, CD86 and MHC II marker expression and interleukin (IL)-12, IL-6 and tumor necrosis factor (TNF)-secretion by DC were enhanced in 3D cultures in comparison to by cells maintained in the 2D states. The data also showed that DNA/chitosan NP activated DC more than LPS in the 3D system. T-Lymphocyte proliferation was induced to a greater extent by DNA/NP-treated DC when both cell types were maintained on the scaffolds. Interestingly, while DC induction of T-lymphocyte interferon (IFN)-and IL-4 release was enhanced in the 3D system (relative to controls), there was a suppression of transforming growth factor (TGF)-production; effects on IL-10 secretion were variable. The results here suggested that collagen-chitosan scaffolds could provide a proinflammatory and activator environment to perform studies to analyze effects of exogenous agents on the induction of DC maturation, NP uptake and/or cytokines release, as well as for the ability of these cells to potentially interact with other immune system cells in vitro.

show abstract

Section: Discussionmentioning

confidence: 56%

Effects of 3-dimensional culture conditions (collagen-chitosan nano-scaffolds) on maturation of dendritic cells and their capacity to interact with T-lymphocytes

Daneshmandi

Dibazar

Fateh

2015

Journal of Immunotoxicology

View full text Add to dashboard Cite

show abstract

“…Researchers are interested in the early event -- from a few hours to one or two days after the injury -- when epithelial cells move toward the wound to form a barrier between the wound and the environment, thereby preventing bacterial infection and interstitial fluid outflow. In the past, diverse efforts have been undertaken to promote wound healing effect: (i) discovery of drugs, growth factors, or cytokines to induce epithelial cell proliferation and migration [1–2]; (ii) development of biocompatible materials as a substrate for epithelial cell movement and wound closure [3–4]. Recently, with the development of microfabrication technology, a number of new materials with features at the micro- or nano-scale have been proposed to accelerate the migration of epithelial cells and thus promote the wound healing process [3–4].…”

Section: Introductionmentioning

confidence: 99%

“…In the past, diverse efforts have been undertaken to promote wound healing effect: (i) discovery of drugs, growth factors, or cytokines to induce epithelial cell proliferation and migration [1–2]; (ii) development of biocompatible materials as a substrate for epithelial cell movement and wound closure [3–4]. Recently, with the development of microfabrication technology, a number of new materials with features at the micro- or nano-scale have been proposed to accelerate the migration of epithelial cells and thus promote the wound healing process [3–4]. Here we report that by using nanopatterned PDMS and a drug called “Gleevec”(Imatinib) we successfully stimulated the migration of epithelial cells towards the wound, and significantly improved the epithelial wound healing efficiency.…”

Section: Introductionmentioning

confidence: 99%

Nanograting structure promotes lamellipodia-based cell collective migration and wound healing

Chen

Atchison

et al. 2014

2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society

View full text Add to dashboard Cite

Wound healing is a dynamic and complex process of replacing missing or dead cell structures and tissue layers. The aim of this research is to discover biocompatible materials and drugs that can promote cell migration in the wound area and thus enhance desirable wound healing effects. In this paper, we report that PDMS nanogratings could accelerate the migration of epithelial cells along the grating axis, and the addition of Imatinib could further increase the epithelial cell wound healing speed to 1.6 times the speed of control cells. We also demonstrate that this migration is mediated by lamellipodia protrusion, and is Rac1-GTPase activity dependent. Lastly, we discuss the potential application and prospect of different nanostructured biomaterials for wound healing studies.

show abstract

“…Chitosan (CS) is an ideal biological material with a large amount of primary amines and displays many useful properties, such as biocompatibility, biodegradability, and adsorption activity . Therefore, it has been widely used in tissue‐engineering scaffolds, wound healing dressing, and removal of heavy metals.…”

Section: Introductionmentioning

confidence: 99%

Ultrasmall Ag nanocrystals supported on chitosan/PVA nanofiber mats with bifunctional properties

Wang

Wei

et al. 2018

J of Applied Polymer Sci

View full text Add to dashboard Cite

Bifunctional nanofiber mats consisting of chitosan (CS), poly(vinyl alcohol) (PVA), and silver nanocrystals (Ag NCs) have been fabricated by a facile electrospinning method. The formation and presence of Ag NCs supported on CS/PVA nanofibers are confirmed by ultraviolet‐visible spectroscopy and X‐ray diffraction. The morphology of the samples is characterized by transmission electron microscopy and scanning electron microscopy. The prepared Ag NCs/CS/PVA nanofiber mats show pronounced antibacterial activity against Escherichia coli and excellent filtration property for suspended particulate matter (SPM) particles. © 2018 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2018, 135, 46504.

show abstract

Poly (lactic acid)–chitosan–collagen composite nanofibers as substrates for blood outgrowth endothelial cells

Cited by 26 publications

References 9 publications

Effects of 3-dimensional culture conditions (collagen-chitosan nano-scaffolds) on maturation of dendritic cells and their capacity to interact with T-lymphocytes

Effects of 3-dimensional culture conditions (collagen-chitosan nano-scaffolds) on maturation of dendritic cells and their capacity to interact with T-lymphocytes

Nanograting structure promotes lamellipodia-based cell collective migration and wound healing

Ultrasmall Ag nanocrystals supported on chitosan/PVA nanofiber mats with bifunctional properties

Contact Info

Product

Resources

About