Poly-L-Lactic Acid Increases Collagen Gene Expression and Synthesis in Cultured Dermal Fibroblast (Hs68) Through the p38 MAPK Pathway

Kim, Sung-Ae; Kim, Hyo-Seon; Jung, Jinwoong; Suh, Sung-Il; Ryoo, Young Wook

doi:10.5021/ad.2019.31.1.97

Cited by 20 publications

(22 citation statements)

References 8 publications

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“…We found that human fibroblasts (HFF1) when cultured in vitro with 1 mg/mL Sculptra™ and PLLA nanoparticles (concentrations: 30, 100 and 300 µg/mL) did not show a stimulation in the collagen synthesis within 24 h of treatment. These results were consistent with Kim et al, where a stimulation in collagen synthesis was not seen when dermal fibroblasts were cultured in vitro with 0.1% Sculptra for 24 h [ 12 ]. However, when co-culture setting was employed, it was possible to see the effect on collagen stimulation within 24 h of treatment with PLLA nanoparticles.…”

Section: Discussionsupporting

confidence: 92%

“…Since PLLA nanoparticles could not stimulate collagen synthesis in fibroblast single cultures, we attempted to test stimulation of collagen synthesis in fibroblast/macrophage co-cultures at the same PLLA nanoparticle concentrations (30, 100 and 300 µg/mL) and 1 mg/mL Sculptra™. A total of 1 mg/mL Sculptra was chosen as it was shown to stimulate collagen production by Kim et al [ 12 ]. The collagen levels were measured after 24 h of particle treatment.…”

Section: Resultsmentioning

confidence: 99%

“…Although a number of clinical and histological data were reported, the molecular biologic effects of PLLA have rarely been investigated. To our knowledge, the study from Kim et al [ 12 ] is the only one that tried to explore the effect of Sculptra on collagen synthesis and related signaling pathways in cultured dermal fibroblast. However, collagen synthesis is a complex in vivo event.…”

Section: Discussionmentioning

confidence: 99%

“…There are a number of clinical and histological reposts showing the effect of Sculptra in vivo [ 9 , 10 , 11 ]. There is only one study by Kim et al in 2019 reporting the effect of Sculptra on collagen synthesis by fibroblasts (single culture) in vitro [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

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Investigating the Effect of Biomaterials Such as Poly-(l-Lactic Acid) Particles on Collagen Synthesis In Vitro: Method Is Matter

Ray

2020

JFB

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Poly-l-lactic acid (PLLA), a synthetic, biocompatible, biodegradable polymer, has been safely used in several clinical applications in recent decades. Typically, SculptraTM, the commercially injectable PLLA in the form of microparticles, has been used as facial volumizer in the treatment of lipoatrophy in HIV patients. It also has various applications in tissue engineering by improving cell proliferation and adhesion. Sculptra™ can be categorised as a stimulatory filler as it stimulates the synthesis and deposition of fibrous tissue and collagen. Collagen is one of the most significant components of the extracellular matrix and beneficial for the normal physiology. It is also the structural component of a human body. In most of the studies, the effect of Sculptra on collagen synthesis was investigated in vivo and the majority of the data were from clinical and histological reports. There is only one study reporting this effect in vitro using fibroblasts. Here, we investigated whether PLLA in the form of nanoparticles can provide the same effect on collagen synthesis in fibroblasts as Sculptra. We surprisingly found that there was no stimulation of collagen in fibroblasts alone, whereas the co-cultures of fibroblast and macrophage had shown collagen stimulation by PLLA nanoparticles. It is also confirmed that collagen synthesis was caused by fibroblasts but not macrophages. Although further study needs to be conducted to evaluate its mechanism, our findings showed that choosing an appropriate method is essential for investigating the effect of PLLA or other biomaterials on collagen synthesis by fibroblasts in vitro.

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Section: Discussionsupporting

confidence: 92%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Investigating the Effect of Biomaterials Such as Poly-(l-Lactic Acid) Particles on Collagen Synthesis In Vitro: Method Is Matter

Ray

2020

JFB

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“…Later, the remains of the hydrolyzed products of PLLA are subjected to phag-ocytosis by macrophages 6 . PLLA acts as a stimulant for fibroblasts, thereby inducing synthesis of new collagen, which produces the cosmetic result 7 . PLLA use has not always been free of complications.…”

Section: Discussionmentioning

confidence: 99%

Late Onset Foreign Body Reaction due to Poly-L-Lactic Acid Facial Injections for Cosmetic Purpose

2020

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The safety and efficacy of Poly-L-lactic acid (PLLA) as an injectable facial volumizer for the treatment of lipoatrophy and facial rejuvenation has been widely proven. We experienced a remarkable case of deep-seated nodules on both the cheeks of 57-year-old female 18-months after administration of PLLA filler injection for cosmetic purpose and performed a skin biopsy. With hematoxylin and eosin stain, the nodule showed non-caseating granulomas consisting of histiocytes with central foreign bodies in the dermis. This case report represents the late-onset foreign body reaction due to PLLA facial injections.

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Nonsurgical Jaw Contouring

Parsa

Somenek²

2023

Clinics in Plastic Surgery

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Poly-L-Lactic Acid Increases Collagen Gene Expression and Synthesis in Cultured Dermal Fibroblast (Hs68) Through the p38 MAPK Pathway

Cited by 20 publications

References 8 publications

Investigating the Effect of Biomaterials Such as Poly-(l-Lactic Acid) Particles on Collagen Synthesis In Vitro: Method Is Matter

Investigating the Effect of Biomaterials Such as Poly-(l-Lactic Acid) Particles on Collagen Synthesis In Vitro: Method Is Matter

Late Onset Foreign Body Reaction due to Poly-L-Lactic Acid Facial Injections for Cosmetic Purpose

Nonsurgical Jaw Contouring

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