Poly(N-acryloylglycine-acrylamide) Hydrogel Mimics the Cellular Microenvironment and Promotes Neurite Growth with Protection from Oxidative Stress

Neurogenic and angiogenic poly(N-acryloylglycine)-co-(acrylamide)-co-(N-acryloyl-glutamate) hydrogel: preconditioning effect under oxidative stress and use in neuroregeneration

“…Interestingly, in a previous report, the highest neuronal growth was observed on day 14 of incubation using our hydrogels. 17 However, in the present study, an increased signal of axonal growth is observed within 7 days of treatment. This could occur due to the associated excitatory signal of glutamate moieties present in the p(NAG-Ac-NAE) hydrogel.…”

“…In the previous study, we reported on neuroprotective activity. 17 Furthermore, in this work, we revealed that p(NAG- b -A) influences the increase in the G0/G1 and G2/M phases to a higher extent with a 50% decrease in the sub-G1 phase in contrast to the control (Fig. 6).…”

“…In a previous report, we already showed the neuro-protective role of p(NAG- b -A) hydrogel, which was only glycine-based hydrogel. 17 Herein, we modulated the p(NAG- b -A) hydrogel with glutamate and further investigated its neuroprotective activity. We studied the neuroprotective assay at a lower concentration of the p(NAG-Ac-NAE) hydrogel.…”

“…Neuronal progenitor cells (NPCs) are present near blood vessels, which provide guidance to NPCs 38 for neurogenesis. To develop dual properties bearing angiogenesis/neurogenesis imprinted hydrogel, both previously reported p(NAG- b -A) 17 and presently synthesized p(NAG-Ac-NAE) hydrogels were examined for angiogenic potential. The p(NAG-Ac-NAE) hydrogel is composed of a combination of glycine (inhibitory) and glutamate (excitatory) units, while the p(NAG- b -A) hydrogel is the only inhibitory glycine-containing polymer.…”

“…16 Recently, we showed the neurogenic potential of p(NAG- b -A) hydrogel. 17 However, previous studies were limited to neurogenic potential, but it was not explored owing to its angiogenic properties. Similarly, none of these studies have supported the development of glutamate- and glycine-based co-polymeric hydrogels for their dual angiogenic and neurogenic potential.…”