Poly(hydroxyethyl methacrylate) based magnetic nanoparticles for plasmid DNA purification from Escherichia coli lysate

Perçin, Işık; Karakoç, Veyis; Akgöl, Sinan; Aksöz, Erol; Deni̇zli̇, Adil

doi:10.1016/j.msec.2012.02.031

Cited by 41 publications

(20 citation statements)

References 62 publications

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“…Magnetic nanoparticles (MNPs) have been widely used as a solid phase for DNA isolation with taking advantage of ease of handing [4][5][6][7][8]; it can disperse rapidly in the reaction mixtures, and be collected by external magnetic field, so that it is easy to design fully automated systems [9][10][11][12][13]. A number of studies demonstrated DNA isolation using MNPs on which silica-or amine-modifications are well employed to be responsible for DNA adsorption.…”

Section: Introductionmentioning

confidence: 99%

DNA recovery from a single bacterial cell based on electrostatic interaction using amine dendron-modified magnetic nanoparticles

Maeda

Toyoda

Tanaka

et al. 2015

Electrochimica Acta

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Section: Introductionmentioning

confidence: 99%

DNA recovery from a single bacterial cell based on electrostatic interaction using amine dendron-modified magnetic nanoparticles

Maeda

Toyoda

Tanaka

et al. 2015

Electrochimica Acta

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“…High porosity and small diffusion limitations are the other advantages of membranes. The macroporous adsorbents also offer great advantages when studying with viscous media [60][61][62][63]. Another advantage of PHEMA-Cibacron Blue F3GA cryogel discs is that it contains dye affinity ligand Cibacron Blue F3GA.…”

Section: Resultsmentioning

confidence: 99%

Dye affinity cryogels for plasmid DNA purification

Çimen

Yılmaz²,

Perçin

et al. 2015

Materials Science and Engineering: C

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“…Nanosized magnetic particles have even a higher DNA adsorption capacity. Positively charged poly(hydroxyethyl methacrylate‐ N ‐methacryloyl‐( l )‐histidine) magnetic nanoparticles (91.5 nm) can bind 154 mg/g of plasmid DNA . Because of the flexibility of the method proposed in the present work, magnetic ion exchange resins with particle sizes ranging from submicron to millimeter could be effectively prepared in the same way.…”

Section: Discussionmentioning

confidence: 99%

A novel method to prepare magnetic polymer‐based anion exchangers and their application

Lin

Wang

Lin

et al. 2014

J of Applied Polymer Sci

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Magnetic microspheres with ion-exchange features were prepared by applying a swelling and penetration process using polystyrene-divinylbenzene-based anion-exchange resins as starting materials. The polymeric anion-exchange particles were swollen with an aqueous solution of N-methyl-2-pyrrolidone, followed by incubation with superparamagnetic iron oxide nanoparticles to allow them to penetrate into the swollen particles. The pH value in the solution of magnetic nanoparticles could significantly influence the uptake of magnetic nanoparticles by the swollen anion-exchange particles. Higher amounts of magnetic nanoparticles entrapped within anion exchangers could be achieved at pH 10-12. An increase in the concentration of magnetic nanoparticles led to a higher density of magnetic nanoparticles entrapped within the interior of anion exchangers and, thus, higher magnetization of the magnetic anion exchangers. Loading of the magnetic nanoparticles onto the exchanger had no effect on anion-exchange functionality. The utility of the resulting magnetic anion-exchange resins was demonstrated for the isolation of plasmid pEGFP-C1 from Escherichia coli cell lysates. The magnetic anion-exchange microspheres could be easily collected within a few seconds in a magnetic field. Thus, automation of the protocol for DNA isolation using these magnetic anion-exchange resins has a high potential. V C 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014, 131, 40725.

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Poly(hydroxyethyl methacrylate) based magnetic nanoparticles for plasmid DNA purification from Escherichia coli lysate

Cited by 41 publications

References 62 publications

DNA recovery from a single bacterial cell based on electrostatic interaction using amine dendron-modified magnetic nanoparticles

DNA recovery from a single bacterial cell based on electrostatic interaction using amine dendron-modified magnetic nanoparticles

Dye affinity cryogels for plasmid DNA purification

A novel method to prepare magnetic polymer‐based anion exchangers and their application

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