Poly(glycidyl methacrylate‐<i>co</i>‐3‐thienyl‐methylmethacrylate) based working electrodes for hydrogen peroxide biosensing

Ozoner, Seyda Korkut

doi:10.1002/jctb.2695

Cited by 7 publications

(3 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Electrode modication aiming for enhanced electric conductivities and enlarged surface areas has drawn research attention in the past. [11][12][13][14] These efforts have oen involved coating nanostructured functional materials by physical means onto the surface of a common electrode substrate, typically commercial glass carbon electrode (GCE). One critical issue for such practice is the poor interfacial quality between the coated layer and the host substrate.…”

Section: Introductionmentioning

confidence: 99%

Porous fluorine-doped tin oxide as a promising substrate for electrochemical biosensors—demonstration in hydrogen peroxide sensing

et al. 2014

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Porous fluorine-doped tin oxide as a promising substrate for electrochemical biosensors—demonstration in hydrogen peroxide sensing

et al. 2014

View full text Add to dashboard Cite

“…In this study, no enzyme leakage was determined using the modified method of Lewis and Schuster's protein assay 29 as a result of strong chemical bonding between enzyme and copolymer. 19,30 The electropolymerization CV of the final copolymeric film [poly(GMA-co-MTM)/FDH/PPy], constructed according to the procedure described, was obtained through the copolymerization of poly(GMA-co-MTM) and pyrrole. The typical polymerization CV of pyrrole is clearly seen in Fig.…”

Section: Colorimetric Formaldehyde Analysis (Nash Method)mentioning

confidence: 99%

Electrochemical biosensor for detection of formaldehyde in rain water

Ozoner

Erhan

Yılmaz

et al. 2012

J of Chemical Tech & Biotech

Self Cite

View full text Add to dashboard Cite

BACKGROUD: This study describes the construction of an electrochemical formaldehyde biosensor based on poly(glycidyl methacrylate‐co‐3‐methylthienyl methacrylate)/formaldehyde dehydrogenase/polypyrrole [poly(GMA‐co‐MTM)/FDH/PPy] composite film electrode. Formaldehyde dehydrogenase (FDH) was chemically immobilized via the epoxy groups of the glycidyl methacrylate (GMA) side chain of the polymer. Formaldehyde measurements were conducted in 0.1 mol L−1, pH 8 phosphate buffer solution (PBS) including 0.1 mol L−1 KCl, 0.5 mmol L−1 of NAD+ (cofactor of the enzyme) and 1 mmol L−1 of 1,2‐napthoquinone‐4‐sulfonic acid sodium salt (NQS) as mediator with an applied potential of − 0.23 V (vs. Ag/AgCl, 3 mol L−1 NaCl). Analytical parameters of the biosensor were calculated and discussed. The biosensor was tested in rain water samples. RESULTS: Sensitivity was found to be 15 000 per mmol L−1 (500 nA ppm−1) in a linear range between 0.1 ppm and 3 ppm (3.3–100 µmol L−1). A minimum detectable concentration of 4.5 ppb (0.15 µmol L−1) (S/N = 3) with a relative standard deviation (RSD) of 0.73% (n = 5) was obtained from the biosensor. Response time of the biosensor was very short, reaching 99% of its maximum response in about 4 s. The biosensor was also tested for formaldehyde measurements in rain water samples. Formaldehyde concentrations in samples were calculated using the proposed biosensor with recovery values ranged between 92.2 and 97.7% in comparison with the colorimetric Nash method. CONCLUSION: The poly(GMA‐co‐MTM)/FDH/PPy) electrode showed excellent measurement sensitivity in comparison with other formaldehyde biosensor studies. Strong chemical bonding between the enzyme and the copolymer was created via the epoxy groups of the composite film. The proposed biosensor could be used successfully in rain waters without a pretreatment step. © 2012 Society of Chemical Industry

show abstract

“…The enzyme catalyzes hydrogen peroxide to form the intermediate enzyme contains (Fe 4 + = O). The intermediate 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 horseradish peroxidase has catalytic activity and it gains one electron from the electrode to form another intermediate enzyme, which is subsequently reduced back to the native enzyme by accepting another electron from the electrode under a favorable applied potential [20]. Sometimes, electrical communication between the electrode surface and enzyme is hindered [21] depending on polymeric film morphology which results in signal loss of the biosensor.…”

Section: Electrochemical Response Of the Rhodium Deposited Poly(styrementioning

confidence: 99%

Novel Enzymatic Rhodium Modified Poly(styrene‐g‐oleic amide) Film Electrode for Hydrogen Peroxide Detection

2017

View full text Add to dashboard Cite

Newly synthesized poly(styrene‐g‐oleic amide) was coated onto a rhodium nanoparticle modified glassy carbon (GC) surface for the fabrication of horseradish peroxidase based biosensor used for hydrogen peroxide detection. The rhodium modifed electrode presented ten times higher signal than unmodified electrode even at low elecrtroactive enzyme quantity by enhancing the electron transfer rate at the applied potential of −0.65 V. The biosensor designed by under the optimized rhodium electrodeposition time exhibited a fast response less than 5 s, an excellent operational stability with a relative standard deviation of 0.6 % (n=6), an accuracy of 96 % and a large linear range between 50 μM and 120 mM for hydrogen peroxide. Detection limit and the sensitivity parameters were calculated to be 44 μM and 57 μA mM−1 cm−2, respectively by preserving its entire initial response up to the 15 days, while only 20 % of its initial response was lost at the end of one month.

show abstract

Poly(glycidyl methacrylate‐co‐3‐thienyl‐methylmethacrylate) based working electrodes for hydrogen peroxide biosensing

Cited by 7 publications

References 42 publications

Porous fluorine-doped tin oxide as a promising substrate for electrochemical biosensors—demonstration in hydrogen peroxide sensing

Porous fluorine-doped tin oxide as a promising substrate for electrochemical biosensors—demonstration in hydrogen peroxide sensing

Electrochemical biosensor for detection of formaldehyde in rain water

Novel Enzymatic Rhodium Modified Poly(styrene‐g‐oleic amide) Film Electrode for Hydrogen Peroxide Detection

Contact Info

Product

Resources

About