Poly(glycidyl methacrylate‐divinylbenzene) based immobilized pH gradient capillary isoelectric focusing coupling with MALDI mass spectrometry for enhanced neuropeptide analysis

Zhang, Zichuan; Wang, Junhua; Hui, Limei

doi:10.1002/elps.201100447

Cited by 19 publications

(15 citation statements)

References 30 publications

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“…Immobilization of the ampholytes as reported by Zhang et al. and Wang et al. may be a possible solution.…”

Section: Principle Of Ce Separation and Modesmentioning

confidence: 99%

“…Although CIEF-MS has already been applied in many studies, several limitations like interference of polyampholytes with MS raise the need for adjustment of CIEF-MS protocols for the analysis of proteins/peptides [18]. Immobilization of the ampholytes as reported by Zhang et al [19] and Wang et al [20] may be a possible solution. Additionally, precipitation of the proteins can be a major problem in CIEF-MS.…”

Section: Ciefmentioning

confidence: 99%

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Clinical applications of capillary electrophoresis coupled to mass spectrometry in biomarker discovery: Focus on bladder cancer

Latosinska

Frantzi

Vlahou

et al. 2013

Proteomics Clinical Apps

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A major requirement in the application of proteins as clinical biomarkers is that they provide a highly sensitive and specific result in disease assessment. Since single biomarkers are generally of limited accuracy, a group or panel of well characterized biomarkers appears appropriate, providing a more robust and sensitive mass-spectrometry-based analytical platform. Capillary electrophoresis coupled to MS has been successfully used in biomarker discovery and application, as it enables the selective detection of peptides and small proteins, combining the high separation capacity of CE with the advanced sensitivity of MS. CE-MS allows the characterization of highly complex samples (such as urine, plasma and other biofluids) in a consistent and reproducible way. It has a range of applications, many focusing especially in studies on urinary peptide biomarkers in kidney and cardiovascular diseases. Another major field of interest has been malignancy of the genitourinary system. In the first part of this review we cover technical aspects and performance characteristics of CE-MS, with special focus on the requirements for biomarker discovery and clinical application. In the second part, we review the potential and development of CE-MS in the management of genitourinary cancers, especially bladder cancer (BCa). CE-MS has been employed in several studies aimed at discovering biomarkers for BCa that may be useful in diagnosis, monitoring for recurrence, and prediction of the risk for the muscle-invasive stage. In the last part of the review we discuss current challenges and provide an outlook for ongoing and possible future developments.

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“…Immobilization of the ampholytes as reported by Zhang et al. and Wang et al. may be a possible solution.…”

Section: Principle Of Ce Separation and Modesmentioning

confidence: 99%

Section: Ciefmentioning

confidence: 99%

Clinical applications of capillary electrophoresis coupled to mass spectrometry in biomarker discovery: Focus on bladder cancer

Latosinska

Frantzi

Vlahou

et al. 2013

Proteomics Clinical Apps

View full text Add to dashboard Cite

show abstract

“…Although these data do not apply to CIEF per se, surely if IPGs in a capillary format could be properly developed, CIEF could become a valid tool in proteome analysis. At present the results have not been outstanding, but the field is under continuous development , so there might be hope in the future.…”

Section: Ce Of Peptidesmentioning

confidence: 99%

Capillary electrophoresis and isoelectric focusing in peptide and protein analysis

Righetti¹,

2012

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CZE and CIEF of proteins have preceded, and accompanied, the birth of proteomics. Although they might not be fully exploited in massive proteomic analyses (especially those projects aiming at a deep discovery of possibly the entire proteome of a cell or subcellular organelles or biological fluids), it still has interesting features and advantages, especially with samples of limited heterogeneity and in the field of purity checking for recombinant DNA proteins meant for human consumption. The purpose of this tutorial paper is to guide the reader through the history of the field, then through the main steps of the process, from sample preparation to analysis of proteins and peptides, while commenting on the constraints and caveats of the technique. The tutorial ends with an outlook on the future, which might be dominated by microchip electrophoresis, especially for ultrafast analyses of protein samples in a sieving mode, in presence of either sieving liquid polymers or firm gels polymerized within the microchannels. To this purpose, commercial instrumentation is already available on the market. This tutorial is part of the International Proteomics Tutorial Programme (IPTP 13).

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“…CE is equally suitable for separation of neuropeptides that are zwitterions and whose overall charge is a function of the pH of its surroundings. Capillary isoelectric focusing can separate peptides based on their isoelectric points, and has been used for the characterization of crustacean neuropeptides (Zhang et al, 2012) and human cerebrospinal fluid (Ye et al, 2011).…”

Section: Microseparation Methodsmentioning

confidence: 99%

Small-Volume Analysis of Cell–Cell Signaling Molecules in the Brain

Romanova

Aerts

Croushore

et al. 2013

Neuropsychopharmacol

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Modern science is characterized by integration and synergy between research fields. Accordingly, as technological advances allow new and more ambitious quests in scientific inquiry, numerous analytical and engineering techniques have become useful tools in biological research. The focus of this review is on cutting edge technologies that aid direct measurement of bioactive compounds in the nervous system to facilitate fundamental research, diagnostics, and drug discovery. We discuss challenges associated with measurement of cell-to-cell signaling molecules in the nervous system, and advocate for a decrease of sample volumes to the nanoliter volume regimen for improved analysis outcomes. We highlight effective approaches for the collection, separation, and detection of such small-volume samples, present strategies for targeted and discovery-oriented research, and describe the required technology advances that will empower future translational science.

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Poly(glycidyl methacrylate‐divinylbenzene) based immobilized pH gradient capillary isoelectric focusing coupling with MALDI mass spectrometry for enhanced neuropeptide analysis

Cited by 19 publications

References 30 publications

Clinical applications of capillary electrophoresis coupled to mass spectrometry in biomarker discovery: Focus on bladder cancer

Clinical applications of capillary electrophoresis coupled to mass spectrometry in biomarker discovery: Focus on bladder cancer

Capillary electrophoresis and isoelectric focusing in peptide and protein analysis

Small-Volume Analysis of Cell–Cell Signaling Molecules in the Brain

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