Poly(Glutamic Acid) Produced by Bacterial Fermentation

Giannos, Steven A.; Shah, Devang; Gross, Richard A.; Kaplan, David L.; Mayer, Jean

doi:10.1007/978-94-009-2129-0_46

Cited by 14 publications

(9 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This polymer is a component of natto, a traditional fermented food produced in Japan from soy beans. Recent studies have explored possible enhancement of yields of polymer under controlled fermentation conditions [23]. Films can be formed from the polymer subsequent to purification from culture filtrates.…”

Section: Proteinsmentioning

confidence: 99%

Introduction to Biopolymers from Renewable Resources

Kaplan¹

1998

Biopolymers From Renewable Resources

321

311

View full text Add to dashboard Cite

Section: Proteinsmentioning

confidence: 99%

Introduction to Biopolymers from Renewable Resources

Kaplan¹

1998

Biopolymers From Renewable Resources

321

311

View full text Add to dashboard Cite

“…The production of PGA via aerobic fermentation has been studied by several researchers (Hwan Do et al, 2001;Yoon et al, 2000;Ko and Gross, 1998;Cromwick et al, 1996;Kunioka and Goto, 1994;Giannos et al, 1990). Several parameters that affect PGA yield and molecular weight have been investigated, including medium composition, mode of feeding, Bacillus species, fermentation conditions, and fermentation broth viscosity.…”

Section: Literature Reviewmentioning

confidence: 99%

Rheology, oxygen transfer, and molecular weight characteristics of poly(glutamic acid) fermentation by Bacillus subtilis

Richard

Margaritis

2003

Biotech & Bioengineering

View full text Add to dashboard Cite

Poly(glutamic acid) (PGA) is a water-soluble, biodegradable biopolymer that is produced by microbial fermentation. Recent research has shown that PGA can be used in drug delivery applications for the controlled release of paclitaxel (Taxol) in cancer treatment. A fundamental understanding of the key fermentation parameters is necessary to optimize the production and molecular weight characteristics of poly(glutamic acid) by Bacillus subtilis for paclitaxel and other applications of pharmaceuticals for controlled release. Because of its high molecular weight, PGA fermentation broths exhibit non-Newtonian rheology. In this article we present experimental results on the batch fermentation kinetics of PGA production, mass transfer of oxygen, specific oxygen uptake rate, broth rheology, and molecular weight characterization of the PGA biopolymer.

show abstract

“…The effect of pulse feeding on the improvement of γ-PGA production can be understood by relying on previous studies on the biopolymer synthesis by B. licheniformis ATCC 9945 a . 39,40 The central role of glutamate as a production inducer and structural monomer of γ-PGA confirms the importance of its pulse feeding for higher production. Because glutamate is a known limiting substrate for biopolymer synthesis by fermentation during batch culture.…”

Section: Discussionmentioning

confidence: 90%

“…These studies have not investigated the effect of the pulse feeding strategy parameters including time and number of feeding stages, concentration and composition, and amount of feed, on γ-PGA production enhancement. 39,40 Therefore, with their optimization, B. licheniformis ATCC 9945 a is expected to produce much more γ-PGA than what has been reported so far.…”

Section: Introductionmentioning

confidence: 99%

Poly‐γ‐glutamic acid overproduction of Bacillus licheniformis ATCC 9945^a by developing a novel optimum culture medium and glutamate pulse feeding using different experimental design approaches

Ebrahimzadeh Kouchesfahani,

Bahrami,

Babaeipour

2024

Biotech and App Biochem

View full text Add to dashboard Cite

The commercial production of multifunctional, biocompatible, and biodegradable biopolymers such as poly‐γ‐glutamic acid via microbial fermentation requires the development of simple and cheap methods for mass production. This study optimized the poly‐γ‐glutamic acid production of Bacillus licheniformis ATCC 9945a in several steps. At first, the most critical components of the culture medium, including l‐glutamic acid, citric acid, and glycerol, were selected by screening nine factors through the Plackett–Burman experimental design and then were optimized using the response surface method and the central composite design algorithm. Under optimal conditions, the production of poly‐γ‐glutamic acid increased by more than 4.2 times from 11.2 to 47.2 g/L. This is one of the highest production rates of this strain in submerged batch fermentation reported so far using the optimized medium compared to the conventional base medium. A novel and efficient sudden pulse feeding strategy (achieved by a novel one‐factorial statistical technique) of l‐glutamic acid to the optimized medium increased biopolymer production from 47.2 to 66.1 g/L, the highest value reported in published literature with this strain. This simple, reproducible, and cheap fermentation process can considerably enhance the commercial applications of the poly‐γ‐glutamic acid synthesized by B. licheniformis ATCC 9945a.

show abstract

Poly(Glutamic Acid) Produced by Bacterial Fermentation

Cited by 14 publications

References 3 publications

Introduction to Biopolymers from Renewable Resources

Introduction to Biopolymers from Renewable Resources

Rheology, oxygen transfer, and molecular weight characteristics of poly(glutamic acid) fermentation by Bacillus subtilis

Poly‐γ‐glutamic acid overproduction of Bacillus licheniformis ATCC 9945^a by developing a novel optimum culture medium and glutamate pulse feeding using different experimental design approaches

Contact Info

Product

Resources

About

Poly(Glutamic Acid) Produced by Bacterial Fermentation

Cited by 14 publications

References 3 publications

Introduction to Biopolymers from Renewable Resources

Introduction to Biopolymers from Renewable Resources

Rheology, oxygen transfer, and molecular weight characteristics of poly(glutamic acid) fermentation by Bacillus subtilis

Poly‐γ‐glutamic acid overproduction of Bacillus licheniformis ATCC 9945a by developing a novel optimum culture medium and glutamate pulse feeding using different experimental design approaches

Contact Info

Product

Resources

About

Poly‐γ‐glutamic acid overproduction of Bacillus licheniformis ATCC 9945^a by developing a novel optimum culture medium and glutamate pulse feeding using different experimental design approaches