Poly(ethylene glycol) Hydrogels with Tailorable Surface and Mechanical Properties for Tissue Engineering Applications

Patel, Nehal R.; Whitehead, Anna; Newman, Jamie J.; Caldorera‐Moore, Mary

doi:10.1021/acsbiomaterials.6b00233

Cited by 23 publications

(22 citation statements)

References 19 publications

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“…Previously, we demonstrated the generation of biomaterial scaffolds of varying elasticity by implementing tailorable PEG hydrogels. Results showed that our hydrogel platform is compatible with multiple stem cell types, specifically mouse embryonic stem cells, human adipose stem cells and human bone marrow-derived MSCs (hMSCs) [ 17 ]. Here, we further characterize the interactions of our hydrogel platform with hMSCs, presenting an investigation into the specific interactions between hMSCs and our tailorable, affordable and reproducible PEG-based hydrogel platform.…”

Section: Introductionmentioning

confidence: 99%

“…For conciseness, the hydrogels with an elastic modulus of 50–60 kPa are referred to as ‘stiff hydrogels’ and the hydrogels with an elastic modulus of 8–10 kPa are referred to as ‘soft hydrogels’. Expanding on our previous work, here we demonstrate the utilization of our PEG-based hydrogel blends to study the effect of elasticity on the characteristics and differentiation potential of bone marrow-derived MSCs [ 17 ]. We show that the hydrogels of different elasticities produce changes in hMSC morphology and proliferation, which provides support that the platform has the potential to produce changes in hMSC behavior and cell state.…”

Section: Introductionmentioning

confidence: 99%

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Poly (ethylene glycol) hydrogel elasticity influences human mesenchymal stem cell behavior

Whitehead¹,

Barnett²,

Caldorera‐Moore

et al. 2018

Regenerative Biomaterials

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Coordinated investigations into the interactions between biologically mimicking (biomimetic) material constructs and stem cells advance the potential for the regeneration and possible direct replacement of diseased cells and tissues. Any clinically relevant therapies will require the development and optimization of methods that mass produce fully functional cells and tissues. Despite advances in the design and synthesis of biomaterial scaffolds, one of the biggest obstacles facing tissue engineering is understanding how specific extracellular cues produced by biomaterial scaffolds influence the proliferation and differentiation of various cell sources. Matrix elasticity is one such tailorable property of synthetic scaffolds that is known to differ between tissues. Here, we investigate the interactions between an elastically tailorable polyethylene glycol (PEG)-based hydrogel platform and human bone marrow-derived mesenchymal stem cells (hMSCs). For these studies, two different hydrogel compositions with elastic moduli in the ranges of 50–60 kPa and 8–10 kPa were implemented. Our findings demonstrate that the different elasticities in this platform can produce changes in hMSC morphology and proliferation, indicating that the platform can be implemented to produce changes in hMSC behavior and cell state for a broad range of tissue engineering and regenerative applications. Furthermore, we show that the platform’s different elasticities influence stem cell differentiation potential, particularly when promoting stem cell differentiation toward cell types from tissues with stiffer elasticity. These findings add to the evolving and expanding library of information on stem cell–biomaterial interactions and opens the door for continued exploration into PEG-based hydrogel scaffolds for tissue engineering and regenerative medicine applications.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Poly (ethylene glycol) hydrogel elasticity influences human mesenchymal stem cell behavior

Whitehead¹,

Barnett²,

Caldorera‐Moore

et al. 2018

Regenerative Biomaterials

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show abstract

“…The library exhibits a wide range of compressive moduli from 3.57 KPa to 6.4 MPa, which could mimic a wide range of tissues from soft tissue such as brain and adipose tissue to stiffer cancellous bone. 18,22,23 As shown in Figures 6 and 7, the compressive moduli (E) of the hydrogels increased with PEG-DA concentration. Through a combination of prolation and subsequent molecular relaxation by removal of the porogen, decreasing material stiffness was achieved.…”

Section: Physicochemical Characterization Of Crosslinked Hydrogelmentioning

confidence: 76%

Type II Photoinitiator and Tuneable Poly(Ethylene Glycol)-Based Materials Library for Visible Light Photolithography

Yang

Mina

Bas

et al. 2020

Tissue Engineering Part A

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Stereolithography (SL) has several advantages over traditional biomanufacturing techniques such as fused deposition modeling, including increased speed, accuracy, and efficiency. While SL has been broadly used in tissue engineering for the fabrication of three-dimensional scaffolds that can mimic the in vivo environment for cell growth and tissue regeneration, lithographic printing is usually performed on single-component materials cured with ultraviolet light, severely limiting the versatility and cytocompatibility of such systems. In this Color images are available online. study, we report a highly tunable, low-cost photoinitiator system that we used to establish a systematic library of crosslinked materials based on low molecular weight poly(ethylene glycol) diacrylate. We assessed the physicochemical properties, photocrosslinking efficiency, cost performance, and biocompatibility to demonstrate the capability of manufacturing a multimaterial complex tissue scaffold. Stereolithography (SL) has advantages over traditional biomanufacturing techniques, including accuracy and efficiency. While SL has been broadly used for fabricating three-dimensional scaffolds that can mimic the in vivo environment for cell growth and tissue regeneration, lithographic printing is usually performed on single-component materials cured with ultraviolet light, severely limiting the versatility and cytocompatibility of such systems. In this study, we report a highly tunable photoinitiator system and establish a systematic library of crosslinked materials based on poly(ethylene glycol) diacrylate. We assessed the physicochemical properties, photocrosslinking efficiency and biocompatibility to demonstrate the capability of manufacturing a multimaterial complex tissue scaffold.

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“…Biocompatible fibers are useful for spot illumination, but cannot be employed by themselves to target larger tissue areas. Light guiding hydrogels offer great versatility in their optical and mechanical properties [8][9][10] , can be engrafted into the target tissue 11 and are already in use for tissue engineering purposes 12 . Hydrogel waveguides are already being tested in live tissues for applications such as sensing or phototherapies 9,13 .…”

Section: Hydrogels As Implantable Waveguidesmentioning

confidence: 99%

Hydrogels for efficient light delivery in optogenetic applications

Johannsmeier

Torres

Ripken

et al. 2018

Optogenetics and Optical Manipulation 2018

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Light-based therapies have been established for various indications, such as skin conditions, cancer or neonatal jaundice. Advances in the field of optogenetics open up new horizons for light-tissue interactions with an organism-wide impact. Excitable tissues, such as nerve and muscle tissues, can be controlled by light after the introduction of light-sensitive ion channels. Since these organs are generally not easily accessible to illumination in vivo, there is an increasing need for effective biocompatible waveguides for light delivery. These devices not only have to guide and distribute the light as desired with minimal losses, they should also mimic the mechanical properties of the surrounding tissue to ensure compatibility. In this project, we are tuning the properties of hydrogels from poly(ethylene glycol) derivatives to achieve compatibility with muscle tissue as well as optimal light guiding and distribution for optogenetic applications at the heart. The excitation light is coupled into the hydrogel with a biocompatible fiber. Properties of the hydrogel are mainly tuned by monomer length and concentration. Total reflection can be achieved by embedding a fiber-like hydrogel with a high refractive index into a second, low refractive index gel. Different geometries and scattering microparticles are used for light distribution in a flat gel patch. Targeted cell attachment can be achieved by introducing a protein layer to the otherwise bioinert gel. After optimization, the hydrogel may be used to deliver light for the excitation of genetically altered cardiomyocytes for controlled contraction.

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Poly(ethylene glycol) Hydrogels with Tailorable Surface and Mechanical Properties for Tissue Engineering Applications

Cited by 23 publications

References 19 publications

Poly (ethylene glycol) hydrogel elasticity influences human mesenchymal stem cell behavior

Poly (ethylene glycol) hydrogel elasticity influences human mesenchymal stem cell behavior

Type II Photoinitiator and Tuneable Poly(Ethylene Glycol)-Based Materials Library for Visible Light Photolithography

Hydrogels for efficient light delivery in optogenetic applications

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