Poly (ɛ-caprolactone) nanoparticles of carboplatin: Preparation, characterization and in vitro cytotoxicity evaluation in U-87 MG cell lines

Karanam, Vamshikrishna; Marslin, Gregory; Balakumar, K; Vijayaraghavan, C.; Karthik, S.; Tamilselvan, Natarajan; Bhaskar, Balaji; Franklin, Gregory

doi:10.1016/j.colsurfb.2015.04.005

Cited by 38 publications

(13 citation statements)

References 23 publications

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“…The whole set was placed on a magnetic stirrer adjusted at 37 ± 2 • C and 100 rpm. At appropriate time points (1,2,3,4,6,8,10,12,14,16,20,24, and 28 days), 2 mL aliquot was withdrawn from the release medium and the same volume of fresh medium was added back to maintain a constant volume. Withdrawn samples were diluted with acetone/ethyl acetate mixture (1:1, v/v) and the percentage of PP released was determined spectrophotometrically at 323 nm.…”

Section: In Vitro Release Of Pp From Pnmentioning

confidence: 99%

“…Simultaneously, the stabilizer self-assembles around the polymeric nanoparticles directing its hydrophobic tail to the hydrophobic polymer core and hydrophilic head group towards the external aqueous environment. Using ethyl acetate as the organic solvent confers a couple of advantages; (1) ethyl acetate is able to dissolve both the drug and the polymer, (2) ethyl acetate can produce relatively small particles owing to its low interfacial tension and high diffusivity into the aqueous phase enabling efficient polymer dispersion [20]. In this study, three factors; chitosan coating, drug/polymer ratio and stabilizer type were studied and their influence on physicochemical characteristics, drug release pattern and cytotoxicity were investigated.…”

Section: Preparation Of Formulationsmentioning

confidence: 99%

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Long-Acting Paliperidone Parenteral Formulations Based on Polycaprolactone Nanoparticles; the Influence of Stabilizer and Chitosan on In Vitro Release, Protein Adsorption, and Cytotoxicity

et al. 2020

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Long-acting preparations containing the antipsychotic paliperidone for intramuscular injection has drawn considerable attention to achieve harmless long-term treatment. This study aimed to develop paliperidone loaded polycaprolactone (PCL) nanoparticles and investigate the influence of PCL/drug ratio, stabilizer type, and chitosan coating on physicochemical properties, protein adsorption, and cellular toxicity. Results showed that chitosan coating produced enlarged particle sizes, shifted the surface charges from negative into positive and did not influence encapsulation efficiencies. Chitosan coating relatively sustained the drug release especially in pluronic stabilized formulations. Pluronic F127 based formulations exhibited the least protein adsorption (384.3 μg/mL). Chitosan coating of Tween 80 and polyvinyl alcohol stabilized formulations significantly (p < 0.05) increased protein adsorption. Cellular viability was concentration-dependent and negatively affected by stabilizers. All formulations did not show cellular death at 1.56 μg/mL. Inflammatory responses and oxidative stress were less affected by Tween 80 compared with other stabilizers. Chitosan minimized all aspects of cellular toxicity. Collectively, stabilizer type and chitosan coating play critical roles in developing safe and effective long-acting PCL nanoparticles intended for parenteral drug delivery. The coated formulations containing Tween 80 and Pluronic F127 as stabilizers are warranted a future in vivo study to delineate its safety and efficacy profiles.

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Section: In Vitro Release Of Pp From Pnmentioning

confidence: 99%

Section: Preparation Of Formulationsmentioning

confidence: 99%

Long-Acting Paliperidone Parenteral Formulations Based on Polycaprolactone Nanoparticles; the Influence of Stabilizer and Chitosan on In Vitro Release, Protein Adsorption, and Cytotoxicity

et al. 2020

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“…Nanoformulations are gaining significant attention in recent years due to their ability to achieve site-specific action of anticancer drugs at a therapeutically optimal rate [ 21 , 22 ]. Delivery of anticancer drugs in an encapsulated form not only protects the drug against chemical and enzymatic degradation, but also reduces the unintended toxicity towards normal cells.…”

Section: Resultsmentioning

confidence: 99%

Solid Lipid Nanoparticles of Albendazole for Enhancing Cellular Uptake and Cytotoxicity against U-87 MG Glioma Cell Lines

et al. 2017

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Albendazole (ABZ) is an antihelminthic drug used for the treatment of several parasitic infestations. In addition to this, there are reports on the anticancer activity of ABZ against a wide range of cancer types. However, its effect on glioma has not yet been reported. In the present study, cytotoxicity of ABZ and ABZ loaded solid lipid nanoparticles (ASLNs) was tested in human glioma/astrocytoma cell line (U-87 MG). Using glyceryl trimyristate as lipid carrier and tween 80 as surfactant spherical ASLNs with an average size of 218.4 ± 5.1 nm were prepared by a combination of high shear homogenization and probe sonication methods. A biphasic in vitro release pattern of ABZ from ASLNs was observed, where 82% of ABZ was released in 24 h. In vitro cell line studies have shown that ABZ in the form of ASLNs was more cytotoxic (IC50 = 4.90 µg/mL) to U-87 MG cells compared to ABZ in the free form (IC50 = 13.30 µg/mL) due to the efficient uptake of the former by these cells.

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“…polymer. This nanocarrier exhibited an enhanced cellular uptake in both A549 lung and MA148 ovarian tumor cells [56], while that based on poly(ε-caprolactone) was also efficient uptakes and displayed a significant cytotoxicity in U-87 human glioma cell line, without inducing haemolysis [57]. Moreover, carboplatin-loaded apotransferrin and lactoferrin nanoparticles with high encapsulation efficacy exhibited a significantly cellular uptake and sustained intracellular drug retention in retinoblastoma cells [58], while a chitosan-based formulation demonstrated an enhanced antiproliferative effect against MCF-7 breast cancer cell line [59].…”

Section: Such a Polymeric Formulation Was Developed By Loading In Polmentioning

confidence: 99%

Nanoformulation as a Tool for Improve the Pharmacological Profile of Platinum and Ruthenium Anticancer Drugs

Uivaroşi¹,

Olar²,

Badea³

2017

Descriptive Inorganic Chemistry Researches of Metal Compounds

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Cisplatin and analogs are used for the treatment of some type of cancers in combination with organic cytostatics. Also, two ruthenium (III) complexes are in clinical trials as anticancer drugs. In order to overcome toxicity and resistance associated with this therapy and/or enhance stability, a large variety of formulations based on organic, inorganic, or hybrid matrix were developed and tested both in vivo and in vitro. The best results were obtained for systems properly functionalized in order to enhance the metal content and/ or to specific target the tumor tissue through overexpressed receptors.

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Poly (ɛ-caprolactone) nanoparticles of carboplatin: Preparation, characterization and in vitro cytotoxicity evaluation in U-87 MG cell lines

Cited by 38 publications

References 23 publications

Long-Acting Paliperidone Parenteral Formulations Based on Polycaprolactone Nanoparticles; the Influence of Stabilizer and Chitosan on In Vitro Release, Protein Adsorption, and Cytotoxicity

Long-Acting Paliperidone Parenteral Formulations Based on Polycaprolactone Nanoparticles; the Influence of Stabilizer and Chitosan on In Vitro Release, Protein Adsorption, and Cytotoxicity

Solid Lipid Nanoparticles of Albendazole for Enhancing Cellular Uptake and Cytotoxicity against U-87 MG Glioma Cell Lines

Nanoformulation as a Tool for Improve the Pharmacological Profile of Platinum and Ruthenium Anticancer Drugs

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