Poly(dimethylsiloxane) based microchip for DNA electrophoresis*

Liu, Changchun; Cui, Dafu; Wang, Li

doi:10.1080/10020070412331344001

Cited by 3 publications

(2 citation statements)

References 17 publications

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“…Since PDMS and glass have completely different surface charge density, the separation effectiveness of hybrid microchips has been questioned (Ross et al 2001;Sanders 2002). Our previous research also demonstrated that increasing dispersion phenomena had occurred in hybrid PDMS microchip (Liu et al 2004). …”

Section: Structure Of Pdms Microchip With Integrated Electrodesmentioning

confidence: 93%

“…The rigid characteristics can be enhanced and the electrodes can also be integrated in hybrid PDMS microchips made from PDMS replica with separation channels and a flat substrate of different materials, such as glass (Martin et al 2000), polymethylmethacrylate (Ross et al 2001). However, due to the nonuniformity of surface charge density at the walls of the channels, increasing dispersion has been introduced in hybrid microchips (Ross et al 2001;Liu et al 2004).…”

Section: Introductionmentioning

confidence: 99%

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Design and fabrication of poly(dimethylsiloxane) electrophoresis microchip with integrated electrodes

Liu

Cui

2005

Microsyst Technol

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A novel poly(dimethylsiloxane) (PDMS) microchip integrated with platinum electrodes has been designed and fabricated using the Micro-electromechanical-systems (MEMS) technology. Since high voltage electrodes are integrated on the glass wafer using lift-off process, the microchip is a friendly-to-use system that does not need any extra mechanical apparatus for electrode insertion. To improve the sealing of microchip and ensure the uniformity of microchannel material, one PDMS membrane is formed on glass wafer with electrodes by pressing method. In this study, integrated microchip has been demonstrated as a capillary electrophoresis device for amino acids and satisfactory separation was achieved under separation electrical field strengths of 200 V/cm. The overall performance suggests that this novel microchip is advantageous and practical for the fabrication of lab-on-a-chip.

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Section: Structure Of Pdms Microchip With Integrated Electrodesmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Design and fabrication of poly(dimethylsiloxane) electrophoresis microchip with integrated electrodes

Liu

Cui

2005

Microsyst Technol

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Ionic liquid‐assisted PDMS microchannel modification for efficiently resolving fluorescent dye and protein adsorption

Jiang

Wang

2007

Electrophoresis

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Herein, a hybrid system consisting of ionic liquid (IL) and nonionic surfactant has been successfully developed for dynamic modification of PDMS microchips and analyte adsorption such as fluoresent dyes and proteins has been efficiently suppressed. Mutual authentication between microchip electrophoresis and confocal laser scanning microscope was carried out to characterize the multiple novel functions of the IL-containing system and a possible mechanism was raised. Soluble IL used herein not only played the role as supporting electrolyte, but also provided increased EOF in the PDMS microchannel compared with common electrolytes such as phosphate buffer. Due to the high ionic conductivity of IL, on-column field-amplified sample stacking effect was four-fold higher than that without IL. Furthermore, an excellent synergistic effect existed between IL and nonionic surfactant, which enhanced the ability of resolving analyte adsorption to PDMS surface, and was demonstrated in the sensitive and efficient determination of rhodamine B (with detection limit of 8 nM) and a well separated mixture of proteins.

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PDMS microchip coated with polydopamine/gold nanoparticles hybrid for efficient electrophoresis separation of amino acids

et al. 2011

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In this paper, a novel, simple, economical and environmentally friendly method based on in situ chemically induced synthesis strategy was designed and developed for the modification of a poly(dimethylsiloxane) (PDMS) microchip channel with polydopamine/gold nanoparticles (PDA/Au NPs) to create a hydrophilic and biofouling resistant surface. Dopamine as a reductant and a monomer, and HAuCl(4) as an oxidant to trigger dopamine polymerization and the source of metallic nanoparticles, were filled into the PDMS microchannel to yield in situ a well-distributed and robust PDA/Au NP coating. Au NPs were highly and uniformly dispersed in/on the PDA matrix with a narrow size distribution, as verified by scanning electron microscopy and UV-vis spectra. Compared with the native PDMS microchannel, the modified surfaces exhibited much better wettability, high stability and suppressed electroosmotic mobility, and less nonspecific adsorption towards biomolecules. The water contact angle and EOF of PDA/Au NP-coated PDMS microchip were measured to be 13° and 4.17×10(-4) cm(2)/V s, compared to those of 111° and 5.33×10(-4) cm(2)/V s from the native one, respectively. Fast and efficient separations of five amino acids such as arginine, proline, histidine, valine and threonine suggested greatly improved electrophoretic performance of the PDA/Au NP-functionalized PDMS microchips. This one-step procedure offers an effective approach for a biomimetic surface design on microfluidic chips, which is promising in high-throughput and complex biological analysis.

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Poly(dimethylsiloxane) based microchip for DNA electrophoresis*

Cited by 3 publications

References 17 publications

Design and fabrication of poly(dimethylsiloxane) electrophoresis microchip with integrated electrodes

Design and fabrication of poly(dimethylsiloxane) electrophoresis microchip with integrated electrodes

Ionic liquid‐assisted PDMS microchannel modification for efficiently resolving fluorescent dye and protein adsorption

PDMS microchip coated with polydopamine/gold nanoparticles hybrid for efficient electrophoresis separation of amino acids

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