Poly (D,L-lactide-co-glycolide) nanoparticles: Uptake by epithelial cells and cytotoxicity

Nkabinde, Lindiwe A.; Shoba-Zikhali, L. N. N.; Semete-Makokotlela, Boitumelo; Kalombo, Lonji; Swai, Hulda; Grobler, Anne; Hamman, Josias H.

doi:10.3144/expresspolymlett.2014.23

Cited by 15 publications

(15 citation statements)

References 47 publications

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“…Consequently, it may diminish side effects caused by high concentrations and repeated administrations, and improve patient compliance as compared to conventional regimens [20] . Poly(lactic-co-glycolic acid) (PLGA) copolymers [21] have been extensively used in drug delivery systems (DDS) as alternative to improve conventional formulations [21][22][23] . These DDSs allow extended and controlled releases of drugs dispersed into the polymer [24] .…”

Section: Introductionmentioning

confidence: 99%

Influence of PLGA and PLGA-PEG on the dissolution profile of oxaliplatin

et al. 2016

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SbstractOxaliplatin was inserted into polymeric matrices aiming to study the interaction of this drug with these polymers and its capability to diffuse to the environment. Tested polymers were: (1) polyethylene glycol (PEG), (2) poly(lactic-co-glycolic acid) (PLGA), and (3) a copolymer of them (PLGA-PEG). The latter two were synthesized by us using polycondensation in bulk. Oxaliplatin was included in the matrices by the melt mixing process followed by casting. Fourier tran sform infrared spectroscopy (FTIR), proton nuclear magnetic resonance ( 1 H-NMR) and X-ray diffraction (DRX) studies of the polymers were performed proving the obtaining of the desired materials. In addition, the interaction between drug and matrices and the release profile of the oxaliplatin from these matrices were analyzed. Among them, PEG did not control the oxaliplatin release. In turn, PLGA and PLGA-PEG present drug release profiles quite similar. Oxaliplatin was completely released from PLGA and PLGA-PEG in 5 hours, by a relaxation mechanism. There was no evidence of oxaliplatin interaction with the different polymers. In addition, as the PEG improves the biocompatibility and biomasking, obtained results prove the obtaining of a drug release system, which allowed the total use of the drug improving the cancer treatment and even the welfare of the patients.

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Section: Introductionmentioning

confidence: 99%

Influence of PLGA and PLGA-PEG on the dissolution profile of oxaliplatin

et al. 2016

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“…This finding corroborates that PPAA/PLGA blended MPs cause no significant cell toxicity under these conditions. These results are in agreement with others who have reported that particulate formulations containing PLGA have negligible toxicity in vitro …”

Section: Resultsmentioning

confidence: 99%

Formulation and characterization of poly(propylacrylic acid)/poly(lactic‐co‐glycolic acid) blend microparticles for pH‐dependent membrane disruption and cytosolic delivery

Fernando

Lewis

Evans

et al. 2017

J Biomedical Materials Res

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Poly(lactic-co-glycolic acid) (PLGA) is widely used as a vehicle for delivery of pharmaceutically relevant payloads. PLGA is readily fabricated as a nano- or microparticle (MP) matrix to load both hydrophobic and hydrophilic small molecular drugs as well as biomacromolecules such as nucleic acids and proteins. However, targeting such payloads to the cell cytosol is often limited by MP entrapment and degradation within acidic endolysosomes. Poly(propylacrylic acid) (PPAA) is a polyelectrolyte polymer with the membrane disruptive capability triggered at low pH. PPAA has been previously formulated in various carrier configurations to enable cytosolic payload delivery, but requires sophisticated carrier design. Taking advantage of PPAA functionality, we have incorporated PPAA into PLGA MPs as a simple polymer mixture to enhance cytosolic delivery of PLGA-encapsulated payloads. Rhodamine loaded PLGA and PPAA/PLGA blend MPs were prepared by a modified nanoprecipitation method. Incorporation of PPAA into PLGA MPs had little to no effect on the size, shape, or loading efficiency, and evidenced no toxicity in Chinese hamster ovary epithelial cells. Notably, incorporation of PPAA into PLGA MPs enabled pH-dependent membrane disruption in a hemolysis assay, and a three-fold increased endosomal escape and cytosolic delivery in dendritic cells after 2 h of MP uptake. These results demonstrate that a simple PLGA/PPAA polymer blend is readily fabricated into composite MPs, enabling cytosolic delivery of an encapsulated payload. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1022-1033, 2018.

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“…Also, tuneable surface modification of PLGA NPs had been accomplished by Kiss et al in 2013 [84]. Rhodamine-6G labelled PLGA NPs were prepared by a double emulsion solvent evaporation freeze-drying method by Nkabine et al [85]. The obtained PLGA NPs were non-cytotoxic to three different mammalian cell lines, which confirmed their safe usage as drug carrier systems.…”

Section: Application Of Biodegradable Nanostructure Polymersmentioning

confidence: 99%

Polymeric nanoparticles: Recent development in synthesis and application

Mallakpour¹,

Behranvand²

2016

Express Polym. Lett.

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Abstract. Nanosized particles have attractive characteristics, which have received considerable attention in the last decade. Polymeric nanoparticles (PNP)s are solid particles or particulate dispersions with size in the range of 10-1000 nm. Due to the very small size, the surface area is very large so the percentage of atoms or molecules on the surface is significantly increased, which is expected to have extensive applications in various fields such as drug delivery systems, biosensors, catalysts, nanocomposites, agriculture and environment. The aim of the present paper is to critically review enhancement in the field of synthesis and different application of novel PNPs in various area from drug delivery to composite fabrication. Literature sources were mainly taken from the publications of 2011 and later; though, for a basic depiction of the structural principles, older publications have also been cited. Vol.10, No.11 (2016) 895-913 Available online at www.expresspolymlett.com DOI: 10.3144/expresspolymlett.2016.84 * Corresponding author, e-mail: mallak@cc.iut.ac.ir © BME-PT of these methods as low cost and safe ways should be highlighted. Furthermore, their importance according to their applications discusses. We will also show some properties of them such as morphology and biological activity. Keywords: biopolymers, biocomposites, polymeric nanoparticles, drug delivery systems, biosensors eXPRESS Polymer Letters

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Poly (D,L-lactide-co-glycolide) nanoparticles: Uptake by epithelial cells and cytotoxicity

Cited by 15 publications

References 47 publications

Influence of PLGA and PLGA-PEG on the dissolution profile of oxaliplatin

Influence of PLGA and PLGA-PEG on the dissolution profile of oxaliplatin

Formulation and characterization of poly(propylacrylic acid)/poly(lactic‐co‐glycolic acid) blend microparticles for pH‐dependent membrane disruption and cytosolic delivery

Polymeric nanoparticles: Recent development in synthesis and application

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