Poly(cytosine)‐templated Silver Nanoclusters as Fluorescent Biosensor for Highly Sensitive Detection of Uric Acid

Abstract

Uric acid (UA) is an important biomarker in urine and serum samples for early diagnosis. This study re‐ ports a fluorescent biosensor based on Poly(cytosine)‐templated silver nanoclusters (C‐Ag NCs) and uricase for the highly sensitive and fast detection of UA. The strong fluorescence of the C‐Ag NCs prepared from poly (cytosine) nucleotides templates could be sensitively quenched by trace amount of H2O2, which produced from oxidation reaction of UA catalyzed by uricase. This biosensor exhibits two linear ranges as 50 nM∼50 μM and 50 μM∼400 μM, with a detection limit of 50 nM. The sensitivity of the biosensor is considerably improved compared with the methods reported in the literature. Furthermore, the detection ability of uric acid in serum samples is confirmed and this C‐Ag NCs‐based uric acid biosensor shows good promise of practical application.

Recent advances in nanomaterials‐based optical sensors for detection of various biomarkers (inorganic species, organic and biomolecules)

“…The detection limit observed was 50 nM which was better than other reported methods with a wider range of 50 nM to 400 μM. [ 144 ]…”

“…The detection limit observed was 50 nM which was better than other reported methods with a wider range of 50 nM to 400 μM. [144] Enzymatic fluorescent biosensor was fabricated based on sol-gel encapsulated CdS QDs (QDs)-Uricase/HRP for the identification of uric acid. Enzymes oxidized uric acid to carbon dioxide (CO 2 ), allantoin, and H 2 O 2 .…”