Poly(carboxybetaine) nanomaterials enable long circulation and prevent polymer-specific antibody production

Yang, Wei; Liu, Sijun; Bai, Tao; Keefe, Andrew J.; Zhang, Lei; Ella‐Menye, Jean‐Rene; Li, Yuting; Jiang, Shaoyi

doi:10.1016/j.nantod.2014.02.004

Cited by 152 publications

(147 citation statements)

References 27 publications

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“…Current methods of characterizing nano-and microparticle synthesis and surface modifications include surface enhanced Raman spectroscopy (SERS), 15 Fourier transform infrared spectroscopy (FTIR), 16,17 electron microscopy, 18,19 ultraviolet-visible spectroscopy (UV-Vis), 20 dynamic light scattering (DLS), 15,21 and nanoparticle tracking analysis (NTA). Of these techniques, DLS is the most commonly used, 22 measuring particles from the micrometer range down to sub-nanometer sizes.…”

Section: Introductionmentioning

confidence: 99%

Physical characterization of nanoparticle size and surface modification using particle scattering diffusometry

Clayton¹,

Salameh

Wereley³

et al. 2016

Biomicrofluidics

236

166

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As the field of colloidal science continues to expand, tools for rapid and accurate physiochemical characterization of colloidal particles will become increasingly important. Here, we present Particle Scattering Diffusometry (PSD), a method that utilizes dark field microscopy and the principles of particle image velocimetry to measure the diffusivity of particles undergoing Brownian motion. PSD measures the diffusion coefficient of particles as small as 30 nm in diameter and is used to characterize changes in particle size and distribution as a function of small, labelfree, surface modifications of particles. We demonstrate the rapid sizing of particles using three orders-of-magnitude less sample volume than current standard techniques and use PSD to quantify particle uniformity. Furthermore, PSD is sensitive enough to detect biomolecular surface modifications of nanometer thickness. With these capabilities, PSD can reliably aid in a wide variety of applications, including colloid sizing, particle corona characterization, protein footprinting, and quantifying biomolecule activity. Published by AIP Publishing. [http://dx

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Section: Introductionmentioning

confidence: 99%

Physical characterization of nanoparticle size and surface modification using particle scattering diffusometry

Clayton¹,

Salameh

Wereley³

et al. 2016

Biomicrofluidics

236

166

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“…injections (23). These findings motivated us to test polymer-protein constructs using PCB as a minimally-or nonimmunogenic alternative to PEGylation.…”

mentioning

confidence: 99%

Zwitterionic gel encapsulation promotes protein stability, enhances pharmacokinetics, and reduces immunogenicity

Zhang

Sun

Tsao

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

240

187

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Advances in protein therapy are hindered by the poor stability, inadequate pharmacokinetic (PK) profiles, and immunogenicity of many therapeutic proteins. Polyethylene glycol conjugation (PEGylation) is the most successful strategy to date to overcome these shortcomings, and more than 10 PEGylated proteins have been brought to market. However, anti-PEG antibodies induced by treatment raise serious concerns about the future of PEGylated therapeutics. Here, we demonstrate a zwitterionic polymer network encapsulation technology that effectively enhances protein stability and PK while mitigating the immune response. Uricase modified with a comprehensive zwitterionic polycarboxybetaine (PCB) network exhibited exceptional stability and a greatly prolonged circulation half-life. More importantly, the PK behavior was unchanged, and neither anti-uricase nor anti-PCB antibodies were detected after three weekly injections in a rat model. This technology is applicable to a variety of proteins and unlocks the possibility of adopting highly immunogenic proteins for therapeutic or protective applications.protein delivery | immune response | zwitterionic polymer | hydrogel | nanomedicine

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“…Redox-responsive disassembly; ultralow cell association properties [138] PCB PCB-gold NPs prepared via SI-ATRP Prolonged circulation time; negligible production of polymer-specific antibodies [139] HPMA Polymer-based particles generated via self-assembly process Possible to conjugate with different therapeutic compounds; minimized interactions with plasma proteins; prolonged circulation time [140] POEGMA Replica particles prepared using surfaceinitiated polymerization in MS templates…”

Section: Reducing Protein Adsorptionmentioning

confidence: 99%

“…[154] Zwitterionic poly(carboxybetaine) (PCB)-based nanomaterials, as demonstrated by Yang et al, showed prolonged circulation time after both the first and the second administration, indicating negligible production of polymer-specific Abs, which can be an issue for PEG. [139,151] This indicated that PCB could be an alternative to PEG to resist activation of immune responses as well as rapid clearance from blood circulation. [139] Many other synthetic or biological molecules, including poly[N -(2-hydroxypropyl)-methacrylamide], poly(vinyl alcohol), poly(4-acryloylmorpholine), poly(amino acids), and polysaccharides, have also been explored as alternative choices to PEG to develop stealth drug carriers (Table 2).…”

Section: Reducing Protein Adsorptionmentioning

confidence: 99%

“…[139,151] This indicated that PCB could be an alternative to PEG to resist activation of immune responses as well as rapid clearance from blood circulation. [139] Many other synthetic or biological molecules, including poly[N -(2-hydroxypropyl)-methacrylamide], poly(vinyl alcohol), poly(4-acryloylmorpholine), poly(amino acids), and polysaccharides, have also been explored as alternative choices to PEG to develop stealth drug carriers (Table 2). [140a,142,143,145a,155] Recently, Rao et al exploited red blood cell (RBC) membranes for coating of upconversion particles to avoid protein adsorption from the biological environment.…”

Section: Reducing Protein Adsorptionmentioning

confidence: 99%

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Particle Targeting in Complex Biological Media

Dai

Bertleff‐Zieschang

Braunger

et al. 2017

Adv Healthcare Materials

102

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Over the past few decades, nanoengineered particles have gained increasing interest for applications in the biomedical realm, including diagnosis, imaging, and therapy. When functionalized with targeting ligands, these particles have the potential to interact with specific cells and tissues, and accumulate at desired target sites, reducing side effects and improve overall efficacy in applications such as vaccination and drug delivery. However, when targeted particles enter a complex biological environment, the adsorption of biomolecules and the formation of a surface coating (e.g., a protein corona) changes the properties of the carriers and can render their behavior unpredictable. For this reason, it is of importance to consider the potential challenges imposed by the biological environment at the early stages of particle design. This review describes parameters that affect the targeting ability of particulate drug carriers, with an emphasis on the effect of the protein corona. We highlight strategies for exploiting the protein corona to improve the targeting ability of particles. Finally, we provide suggestions for complementing current in vitro assays used for the evaluation of targeting and carrier efficacy with new and emerging techniques (e.g., 3D models and flow‐based technologies) to advance fundamental understanding in bio‐nano science and to accelerate the development of targeted particles for biomedical applications.

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Poly(carboxybetaine) nanomaterials enable long circulation and prevent polymer-specific antibody production

Cited by 152 publications

References 27 publications

Physical characterization of nanoparticle size and surface modification using particle scattering diffusometry

Physical characterization of nanoparticle size and surface modification using particle scattering diffusometry

Zwitterionic gel encapsulation promotes protein stability, enhances pharmacokinetics, and reduces immunogenicity

Particle Targeting in Complex Biological Media

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