Abstract:Electrochemiluminescence (ECL) of poly‐amidoamine (PAMAM) dendrimers functionalized to Fe3O4 nanoparticles (PAMAM@Fe3O4 NPs) based on Ru(bpy)32+ complex is reported. Different generations of PAMAM dendrimer consisted of 5 (D5), 10 (D10), 8 (D8) and 16 (D16) surface primary amine‐terminated groups. D5@Fe3O4 NPs enhanced ECL emission in comparison to others. A possible mechanism is elucidated based on structural functionality of PAMAM with respect to amines, carbonyl, hydroxyl groups and size‐based diffusion. EC… Show more
“…The intrinsic properties of dendrimers, such as a substantial surface area, abundant modifiable functional groups, considerable loading capacity, distinctive drug release kinetics, and dendritic formation, inspire researchers to graft or cultivate dendrimers on other nanostructures, thereby enabling the development of multifunctional complexes [ 54 ]. Nanostructures can be interconnected by dendrimers, enabling the fabrication of intricate structures that can be used for a wide variety of applications, including biosensing and theranostics [ [75] , [76] , [77] ]. Moreover, dendrimers are capable of altering the optical properties of specific nanostructures, such as QDs, resulting in unique properties such as fluorescence quenching and FRET phenomena [ 78 ].…”
Section: Intentions Of Fabricating Inp-cored Dendrimersmentioning
confidence: 99%
“…PAMAM@Fe 3 O 4 presented an efficient clinical diagnostic method, showcasing enhanced ECL with low concentration. The magnetic property of the Fe 3 O 4 core provides external magnetic control, contributing to the sensitivity and specificity of biosensing [ 75 ]. Fig.…”
“…The intrinsic properties of dendrimers, such as a substantial surface area, abundant modifiable functional groups, considerable loading capacity, distinctive drug release kinetics, and dendritic formation, inspire researchers to graft or cultivate dendrimers on other nanostructures, thereby enabling the development of multifunctional complexes [ 54 ]. Nanostructures can be interconnected by dendrimers, enabling the fabrication of intricate structures that can be used for a wide variety of applications, including biosensing and theranostics [ [75] , [76] , [77] ]. Moreover, dendrimers are capable of altering the optical properties of specific nanostructures, such as QDs, resulting in unique properties such as fluorescence quenching and FRET phenomena [ 78 ].…”
Section: Intentions Of Fabricating Inp-cored Dendrimersmentioning
confidence: 99%
“…PAMAM@Fe 3 O 4 presented an efficient clinical diagnostic method, showcasing enhanced ECL with low concentration. The magnetic property of the Fe 3 O 4 core provides external magnetic control, contributing to the sensitivity and specificity of biosensing [ 75 ]. Fig.…”
“…Various imaging techniques have been used in HCC detection, such as Fluorescence microscopy [23,24], Positron Emission Computed Tomography (PET) [25], Computed Tomography (CT) [26], and Magnetic Resonance Imaging (MRI) [27,28]. Among them, fluorescence imaging has been a powerful tool for the in situ real-time detection of biomarkers owing to its good sensitivity and selectivity in living systems.…”
Section: Introductionmentioning
confidence: 99%
“…However, GPC-3 is less or not expressed in tissues with most other cancers [21,22], indicating that it can help to distinguish HCC from benign liver nodules and has obvious sensitivity and specificity for the diagnosis of HCC, thus can be used as a marker for identifying HCC-specific tumors. Various imaging techniques have been used in HCC detection, such as Fluorescence microscopy [23,24], Positron Emission Computed Tomography (PET) [25], Computed Tomography (CT) [26], and Magnetic Resonance Imaging (MRI) [27,28]. Among them, fluorescence imaging has been a powerful tool for the in situ real-time detection of biomarkers owing to its good sensitivity and selectivity in living systems.…”
Hepatocellular carcinoma (HCC) is a malignant tumor with high morbidity and mortality on a global scale, and the development of accurate detection and imaging methods for HCC cells is urgently needed. Herein, by connecting peptide L5, which can specifically bind to the overexpressed Glypican-3 (GPC-3) protein of HCC cells with aggregation-induced emission (AIE) moiety ammonium cation-functionalized 9,10-distyrylanthracene (NDSA) via the “click” reaction, we synthesized a fluorescent probe NDSA-L5. In an aqueous solution, the probe shows weak emission, whereas, in the presence of the GPC-3 protein, bright fluorescence can be obtained since NDSA-L5 binds to the GPC-3 protein, leading to the restricted intramolecular movement of AIE-active NDSA-L5. The imaging and flow cytometry experiments demonstrate that the NDSA-L5 probe can rapidly accumulate in the subcutaneous HCC cells and liver tumor tissue and shows a potential application in early detection and surgical navigation for HCC cancer.
“…Therefore, seeking a simple, accurate, and sensitive detection method for AFP is very important for clinical diagnosis. In this context, there have been a number of techniques developed for the specific recognition of AFP, mainly including electrochemiluminescent assay [2], surface plasmon resonance [3], and enzyme-linked immunosorption assay [4]. In comparison with aforementioned techniques which are time consuming and need sophisticated instruments, electrochemical techniques come with many qualities such as simple preparation, low-cost instrumentation, fast performance, high sensitivity, selectivity and can be miniaturized into a portable device.…”
Composite of polyaniline, bovine serum albumin and silver nanoparticles was used as the enriched immobilization matrix (EIM)‐cum‐electrode material for detection of liver cancer biomarker, α‐fetoprotein (AFP) in blood serum. In the sandwich biosensor, signal amplification was bestowed by EIM and detection probe. EIM entrapped large amount of capture antibody due to its higher surface area and amine‐rich nature, whereas detection probe enhanced the electrochemical signal during its binding with AFP. Biosensor exhibited excellent sensitivity, selectivity, reproducibility with a detection limit of 4.7 pg/mL. The amplification strategy adopted herein has potential for clinical detection of cancer biomarkers in point‐of‐care diagnostics.
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