Poly(amido amine) is an ideal carrier of miR-7 for enhancing gene silencing effects on the EGFR pathway in U251 glioma cells

Abstract

microRNAs are regarded as promising drugs for glioma gene therapy. However, conventional administration routes, such as oral administration and intravenous infusion, present low efficiency due to the blood-brain barrier and intercellular retention, thereby limiting their application. Recent studies showed poly(amido amine) (PAMAM) was a candidate carrier due to its high solubilization, delayed release and low toxicity. In the present study, U251 human brain glioma cells were transfected with the miR-7 gene using PAMAM as the vector to determine the transfection efficiency and therapeutic effects in vivo and in vitro. We found that PAMAM exhibited higher transfection efficiency and longer duration of action compared with liposome delivery, and miR-7 efficiently silenced some genes involved in the epidermal growth factor receptor (EGFR) pathway and achieved favorable effects in treating glioma in vivo and in vitro. These investigations provide a basis for developing high-efficiency micromolecular drug delivery.

Circ‐U2AF1 promotes human glioma via derepressing neuro‐oncological ventral antigen 2 by sponging hsa‐miR‐7‐5p

“…Cui et al, , X. Cui et al, ; Da Ros, Gutierrez‐Perez, Ferres‐Marco, & Dominguez, ; Feng et al, ; Higuchi et al, ; Horsham et al, ; Hua et al, ; Huynh & Jones, ). In addition, miR‐7‐5p was reported to exert a tumor‐suppressive function in glioblastoma and glioma by regulation of the EGFR, PI3K/ATK, Raf/MEK/ERK, and IGF‐1R pathways (X. Liu et al, , M. Liu et al, ; W. Wang et al, , B. Wang et al, ). Regulation of miR‐7 by circular RNAs, such as ciRS‐7, has been found as a critical approach to advance our interpretation of numerous tumor etiologies (Hansen, Kjems, & Damgaard, ).…”

Nanocarrier-based therapies for CNS tumors

“…Numerous ligands have been attached to dendrimers, including PEG, transferrin, tamoxifen, rabies virus glycoprotein and angiopep-2, to name a few [61][62][63][64]. These ligands prolong circulation time, reduce immuno genicity, improve BBB bypass, aide in cell targeting and balance particle charge.…”

miRNA nanotherapeutics for cancer

“…Poly(amido amine) (PAMAM) has been found to be an effective carrier for the delivery of miR-7 into glioma cells owing to its low toxicity, high solubilization, and delayed release [49]. A recent study demonstrated the successful delivery of tumor suppressor miRNA (let-7a)-conjugated magnetic NPs to brain cells followed by magnetic hyperthermia.…”