Poly(ADP-ribose) synthesis and cell division

Kidwell, William R.; Burdette, Kristen

doi:10.1016/0006-291x(74)91023-7

Cited by 53 publications

(16 citation statements)

References 6 publications

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“…Thus, the abundance of PARP mRNA (26), poly(ADP-ribose) (27), and poly(ADPribose)-linked acceptor proteins such as histone H1 dimers (28) increases markedly at the S-G 2 transition. Furthermore, chemical inhibitors of PARP also arrest cells at G 2 (29). Consistent with the results of microarray hybridization, a marked decrease in transcript levels in PARP Ϫ/Ϫ cells, as shown by RT-PCR analysis, correlates with decreased abundance of cyclin B1 protein in these cells (Fig.…”

Section: Reduced Expression Of Genes Important In Cell Cycle Regulatisupporting

confidence: 83%

Misregulation of gene expression in primary fibroblasts lacking poly(ADP-ribose) polymerase

Simbulan-Rosenthal

Rosenthal

et al. 2000

Proc. Natl. Acad. Sci. U.S.A.

124

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Poly(ADP-ribose) polymerase (PARP) is implicated in the maintenance of genomic integrity, given that inhibition or depletion of this enzyme increases genomic instability in cells exposed to genotoxic agents. We previously showed that immortalized fibroblasts derived from PARP ؊/؊ mice exhibit an unstable tetraploid population, and partial chromosomal gains and losses in PARP ؊/؊ mice and immortalized fibroblasts are accompanied by changes in the expression of p53, Rb, and c-Jun, as well as other proteins. A tetraploid population has also now been detected in primary fibroblasts derived from PARP ؊/؊ mice. Oligonucleotide microarray analysis was applied to characterize more comprehensively the differences in gene expression between asynchronously dividing primary fibroblasts derived from PARP ؊/؊ mice and their wild-type littermates. Of the 11,000 genes monitored, 91 differentially expressed genes were identified. The loss of PARP results in down-regulation of the expression of several genes involved in regulation of cell cycle progression or mitosis, DNA replication, or chromosomal processing or assembly. PARP deficiency also up-regulates genes that encode extracellular matrix or cytoskeletal proteins that are implicated in cancer initiation or progression or in normal or premature aging. These results provide insight into the mechanism by which PARP deficiency impairs mitotic function, thereby resulting in the genomic alterations and chromosomal abnormalities as well as in altered expression of genes that may contribute to genomic instability, cancer, and aging.

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Section: Reduced Expression Of Genes Important In Cell Cycle Regulatisupporting

confidence: 83%

Misregulation of gene expression in primary fibroblasts lacking poly(ADP-ribose) polymerase

Simbulan-Rosenthal

Rosenthal

et al. 2000

Proc. Natl. Acad. Sci. U.S.A.

124

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“…HeLa cells obtained from Flow Laboratories were maintained in shake cultures as previously described (Kidwell and Burdette, 1974). Periodic testing indicated that the cultures were free of mycoplasma contamination.…”

Section: Methodsmentioning

confidence: 99%

“…The inhibitor may be specific since a variant HeLa cell has been isolated, the growth of which is not sensitive to the inhibitor. Furthermore, the polymerase activity in nuclei of the variant is less sensitive to inhibition by 5-methylnicotinamide than is that of the parental HeLa cell (Kidwell and Burdette, 1974).…”

mentioning

confidence: 87%

“…First, poly(ADP-ribose) polymerase activity peaks a t this time in the cell cycle (Miwa et al, 1973;Kidwell and Watts, 1974) and there is a coincident maximum in the rate of incorporation of [3H]adenosine into the polymer in intact cells (Kidwell and Watts, 1974). Second, an inhibitor of poly(ADP-ribose) polymerase, 5-methylnicotinamide, arrests the growth of HeLa cells between the end of the period of DNA synthesis and the time of mitosis (Kidwell and Burdette, 1974). The inhibitor may be specific since a variant HeLa cell has been isolated, the growth of which is not sensitive to the inhibitor.…”

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confidence: 99%

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Changes in poly(adenosine diphosphate-ribose) and poly(adenosine diphosphate-ribose) polymerase in synchronous HeLa cells

Kidwell¹,

Mage²

1976

Biochemistry

107

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An antibody has been prepared which is highly specific for poly(adenosine diphosphate-ribose). Neither poly(A), DNA, nor a variety of adenine-containing nucleosides or nucleotides were effective in competing with poly(ADP-ribose) for binding to the antibody. Of all compounds tested, only adenosine diphosphate-ribose competed for binding to the antibody. Unlabeled poly(adenosine diphosphate-ribose) was about 10 000 times more effective in competing with labeled polymer for antibody binding than was adenosine diphosphate-ribose. Using the antibody, the amount of poly(adenosine diphosphate-ribose) was found to increase from early S phase to a peak at mid S with a second, even larger increase seen at the S-G2 transition point in synchronously dividing HeLa cells. Pulse labeling of the polymer with [2-3H]adenosine was also maximal at the same time points. Changes in the levels of poly(adenosine diphosphate-ribose) polymerase activity measured in isolated nuclei coincided with the changes in amounts of polymer present in intact cells during progression from S phase into G2.

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“…Recent studies have shown that NO at high concentrations, as well as PN, can activate poly(adenosine 5'-diphos-phoribose) synthase (PARS) [87,89] which consumes energy, resulting in cell death through depletion of beta-nicotinamide adenine dinucleotide (NAD + ) and adenosine triphosphate (ATP) [92,93]. PARS is involved in DNA repair [94,95], cell differentiation [96,97], and control of the cell cycle [98,99]. Recent studies in rat aortic rings exposed to PN demonstrate a dosedependent reduction in vascular contractility, with a significant increase in PARS activity [100].…”

Section: Vasorelaxant Properties Of Peroxyni-tritementioning

confidence: 99%

Potential Benefits of Peroxynitrite

Nossaman

Kadowitz²

2008

TOPHARMJ

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Peroxynitrite (PN) is generated by the reaction of nitric oxide (NO) and superoxide in one of the most rapid reactions in biology. Studies have reported that PN is a cytotoxic molecule that contributes to vascular injury in a number of disease states. However, it has become apparent that PN has beneficial effects including vasodilation, inhibition of platelet aggregation, inhibition of inflammatory cell adhesion, and protection against ischemia/reperfusion injury in the heart. It is our hypothesis that PN may serve to inactivate superoxide and prolong the actions of NO in the circulation. This manuscript reviews the beneficial effects of PN in the cardiovascular system. Keywords:Nitric oxide/*metabolism, nitric oxide synthase/metabolism/physiology, peroxynitrous acid/metabolism, reactive nitrogen species/metabolism, reactive oxygen species/metabolism, endothelium, vascular/drug effects/metabolism, muscle, smooth, vascular/drug effects/*metabolism, vasodilator agents/adverse effects, oxidative stress/*physiology, vasodilation/drug effects.

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Poly(ADP-ribose) synthesis and cell division

Cited by 53 publications

References 6 publications

Misregulation of gene expression in primary fibroblasts lacking poly(ADP-ribose) polymerase

Misregulation of gene expression in primary fibroblasts lacking poly(ADP-ribose) polymerase

Changes in poly(adenosine diphosphate-ribose) and poly(adenosine diphosphate-ribose) polymerase in synchronous HeLa cells

Potential Benefits of Peroxynitrite

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