Poly(A) Tail Shortening of α-Amylase mRNAs in Vegetative Tissues of Oryza sativa

Lue, Ming-Yong; Lee, Hung-Tu

doi:10.1006/bbrc.1994.2032

Cited by 3 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been proposed, based on the examination of mRNA decay products, that ∼25% of the oat phytochrome A mRNA pool degrades via a deadenylationdependent 3Ј → 5Ј pathway (Higgs and Colbert 1994). Poly(A) tail length of the rice ␣-amylase mRNA, ␣Amy3, is regulated during development (Lue and Lee 1994). In a related study, ␣Amy3 3Ј-UTR has been shown to be responsible for destabilizing the ␣Amy3 transcript in highsucrose media, possibly by promoting rapid deadenylation (Chan and Yu 1998).…”

Section: Introductionmentioning

confidence: 99%

mRNA deadenylation by PARN is essential for embryogenesis in higher plants

Reverdatto

Dutko²,

Chekanova³

et al. 2004

RNA

View full text Add to dashboard Cite

Deadenylation of mRNA is often the first and rate-limiting step in mRNA decay. PARN, a poly(A)-specific 3' --> 5' ribonuclease which is conserved in many eukaryotes, has been proposed to be primarily responsible for such a reaction, yet the importance of the PARN function at the whole-organism level has not been demonstrated in any species. Here, we show that mRNA deadenylation by PARN is essential for viability in higher plants (Arabidopsis thaliana). Yet, this essential requirement for the PARN function is not universal across the phylogenetic spectrum, because PARN is dispensable in Fungi (Schizosaccharomyces pombe), and can be at least severely downregulated without any obvious consequences in Metazoa (Caenorhabditis elegans). Development of the Arabidopsis embryos lacking PARN (AtPARN), as well as of those expressing an enzymatically inactive protein, was markedly retarded, and ultimately culminated in an arrest at the bent-cotyledon stage. Importantly, only some, rather than all, embryo-specific transcripts were hyperadenylated in the mutant embryos, suggesting that preferential deadenylation of a specific select subset of mRNAs, rather than a general deadenylation of the whole mRNA population, by AtPARN is indispensable for embryogenesis in Arabidopsis. These findings indicate a unique, nonredundant role of AtPARN among the multiple plant deadenylases.

show abstract

Section: Introductionmentioning

confidence: 99%

mRNA deadenylation by PARN is essential for embryogenesis in higher plants

Reverdatto

Dutko²,

Chekanova³

et al. 2004

RNA

View full text Add to dashboard Cite

show abstract

State of Decay: An Update on Plant mRNA Turnover

Belostotsky

2008

Current Topics in Microbiology and Immunology

View full text Add to dashboard Cite

Regulation of the β-hydroxyacyl ACP dehydratase gene of Picea mariana by alternative splicing

et al. 2006

View full text Add to dashboard Cite

The gene for beta-hydroxyacyl ACP dehydratase, a de novo fatty acid biosynthetic enzyme, was cloned from Picea mariana (black spruce) and consists of five exons and four introns. The first intron of the beta-hydroxyacyl ACP dehydratase mRNA is alternatively spliced. Retention of intron 1 in splice variants results in truncation of the beta-hydroxyacyl ACP dehydratase ORF at a premature termination codon. In addition, splicing of intron 1 was found to be associated with cold temperature. mRNAs retaining intron 1 increase with seed imbibition at 22 degrees C but not 4 degrees C, whereas, splicing of intron 1 increases in winter weeks with temperatures below freezing. These results provide evidence that alternative splicing may also contribute to regulation of lipid biosynthesis in Picea mariana.

show abstract

Poly(A) Tail Shortening of α-Amylase mRNAs in Vegetative Tissues of Oryza sativa

Cited by 3 publications

References 0 publications

mRNA deadenylation by PARN is essential for embryogenesis in higher plants

mRNA deadenylation by PARN is essential for embryogenesis in higher plants

State of Decay: An Update on Plant mRNA Turnover

Regulation of the β-hydroxyacyl ACP dehydratase gene of Picea mariana by alternative splicing

Contact Info

Product

Resources

About